Mycobiology

The Korean Society of Mycology (한국균학회)
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Neuraminidase Inhibitors from the Culture Broth of Phellinus linteus

  • Yeom, Ji-Hee (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Lee, In-Kyoung (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Ki, Dae-Won (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Lee, Myeong-Seok (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Seok, Soon-Ja (National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Yun, Bong-Sik (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University)
  • Received : 2012.05.30
  • Accepted : 2012.06.04
  • Published : 2012.06.30
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Abstract

During the search for neuraminidase inhibitors from medicinal fungi, we found that the culture broth of Phellinus linteus exhibited potent inhibitory activity. Solvent partition, Sephadex LH-20 column chromatography, and high-performance liquid chromatography (HPLC) were performed for purification of two active substances from the culture broth. According to $^1H$ NMR measurements and comparison of HPLC retention times with those of authentic compounds, their chemical structures were identified as hispidin and hypholomine B. Compounds (hispidin) 1 and 2 (hypholomine B) inhibited neuraminidase, with $IC_{50}$ values of 13.1 and 0.03 ${\mu}M$, respectively.

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References

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