Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지
DOI QR코드

DOI QR Code

Suppressive Effects of Fucoxanthin on Degranulation in IgE-antigen complex-stimulated RBL-2H3 Cells

  • NamKoong, Seung (Department of Physical Therapy, Kangwon National University) ;
  • Joo, Hae-Mi (Radiation Health Research Institute, Korea Hydro & Nuclear Power Co., Ltd.) ;
  • Jang, Seon-A (Radiation Health Research Institute, Korea Hydro & Nuclear Power Co., Ltd.) ;
  • Kim, Ye-Jin (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Kim, Tae-Seong (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Sohn, Eun-Hwa (Department of Herbal Medicine Resource, Kangwon National University)
  • Received : 2012.05.30
  • Accepted : 2012.06.25
  • Published : 2012.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The marine carotenoid fucoxanthin can be found in marine brown seaweeds, macroalgae, diatoms, and microalgae, and has remarkable biological properties. Numerous studies have shown that fucoxanthin has considerable potential and promising applications in human health, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. We here investigated the mechanisms by anti-allergic activity of fucoxanthin fraction from Eisenia bicyclis in immunoglobulin E-antigen complex (IgE/DNP-BSA)-stimulated RBL-2H3 mast cells. This study we found that the fucoxanthin inhibits the release of ${\beta}$-hexosaminidase and suppressed not only transcriptional activation of NF-${\kappa}B$, but also phosphorylation of ERK and JNK in IgE/DNP-BSA-treated RBL-2H3 cells. Fucoxanthin may be useful for preventing allergic diseases, including asthma and atopic dermatitis.

Keywords

References

  1. Barnes, P.J. 2008. Role of GATA-3 in allergic diseases. Curr. Mol. Med. 8:330-334. https://doi.org/10.2174/156652408785160952
  2. Ding, A.H., C.F. Nathan and D.J. Stuehr. 1988. Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J. Immunol. 141:2407-2412.
  3. Gilfillan, A.M. and C. Tkaczyk. 2006. Integrated signaling pathways for mast-cell activation. Nat. Rev. Immunol. 6: 218-230. https://doi.org/10.1038/nri1782
  4. Hu, T., D. Liu, Y. Chen, J. Wu and S. Wang. 2010. Antioxidant activity of sulfated polysaccharide fractions extracted from Undaria pinnitafida in vitro. Int. J. Biol. Macromol. 46: 193-198. https://doi.org/10.1016/j.ijbiomac.2009.12.004
  5. Huang, F., K. Yamaki, X. Tong, L. Fu, R. Zhang, Y. Cai, R. Yanagisawa, K. Inoue, H. Takano and S. Yoshino. 2008. Inhibition of the antigen-induced activation of RBL-2H3 cells by sinomenine. Int. Immunopharmacol. 8:502-507. https://doi.org/10.1016/j.intimp.2007.12.009
  6. Joe, M.J., S.N. Kim, H.Y. Choi, W.S. Shin, G.M. Park, D.W. Kang and Y.K. Kim. 2006. The inhibitory effects of eckol and dieckol from Ecklonia stolonifera on the expression of matrix metalloproteinase-1 in human dermal fibroblasts. Biol. Pharm. Bull. 29:1735-1739. https://doi.org/10.1248/bpb.29.1735
  7. Kang, K., H.J. Hwang, D.H. Hong, Y. Park, S.H. Kim, B.H. Lee and H.C. Shin. 2004. Antioxidant and antiinflammatory activities of ventol, a phlorotannin-rich natural agent derived from Ecklonia cava, and its effect on proteoglycan degradation in cartilage explant culture. Res. Commun. Mol. Pathol. Pharmacol. 115-116:77-95.
  8. Karin, M. and A. Lin. 2002. NF-${\kappa}$B at the crossroads of life and death. Nat. Immunol. 3:221-227.
  9. Kim, S.M., Y.F. Shang and B.H. Um. 2011. A preparative method for isolation of fucoxanthin from Eisenia bicyclis by centrifugal partition chromatography. Phytochem. Anal. 22 (4):322-329. https://doi.org/10.1002/pca.1283
  10. Lee, J., S.J. Lee and K.T. Lim. 2012. CTB glycoprotein (75 kDa) inhibits IgE releasing, TNF-${\alpha}$ and IL-6 expressed by bisphenol A in vivo and in vitro. Food Chem. Toxicol. 50 (6):2109-2117. https://doi.org/10.1016/j.fct.2012.03.043
  11. Matsuda, H., Q. Wang, K. Matsuhira, S. Nakamura, D. Yuan and M. Yoshikawa. 2008. Inhibitory effects of thunberginols A and B isolated from Hydrangeae Dulcis Folium on mRNA expression of cytokines and on activation of activator protein- 1 in RBL-2H3 cells. Phytomedicine 15:177-184 https://doi.org/10.1016/j.phymed.2007.09.010
  12. Namkoong, S., S.C. Kang, H. Do, K.H. Jang, S.A. Jang, M.G. Choung and E.H. Sohn. 2011. Immunomodulatory effects of supplementation with extracts from the marine brown alga Eisenia bicyclis on macrophages. J. Korean Plant Res. 24(3): 298-303. https://doi.org/10.7732/kjpr.2011.24.3.298
  13. Naal, R.M., J. Tabb, D. Holowka and B. Baird. 2004. In situ measurement of degranulation as a biosensor based on RBL- 2H3 mast cells. Biosens Bioelectron. 20(4):791-796. https://doi.org/10.1016/j.bios.2004.03.017
  14. Park, H.J., D.H. Jung, H. Joo, N.S. Kang, S.A. Jang, J.G. Lee and E.H. Sohn. 2010. The comparative study of anti-allergic and anti-inflammatory effects by fermented red ginseng and red ginseng. J. Korean Plant Res. 23(5): 393-485 (in Korean).
  15. Peng, J., J.P. Yuan, C.F. Wu and J.H. Wang. 2011. Fucoxanthin, a marine carotenoid present in brown seaweeds and diatoms: metabolism and bioactivities relevant to human health. Mar. Drugs. 9(10):1806-1828. https://doi.org/10.3390/md9101806
  16. Soter, N.A. 1989. Morphology of atopic eczema. Allergy 44 (Suppl 9):16-19. https://doi.org/10.1111/j.1398-9995.1989.tb02449.x
  17. Theoharides, T.C. and D. Kalogeromitros. 2006. The critical role of mast cells in allergy and inflammation. Ann. NY Acad. Sci. 1088:78-99. https://doi.org/10.1196/annals.1366.025
  18. Yang, L., L. Cohn, D.H. Zhang, R. Homer, A. Ray and P. Ray. 1998. Essential role of nuclear factor B in the induction of eosinophilia in allergic airway inflammation. J. Exp. Med. 188:1739-1750. https://doi.org/10.1084/jem.188.9.1739

Cited by

  1. The Effect of Extract from Sea Buckthorn on DNCB-induced Atopic Dermatitis in NC/Nga Mice vol.25, pp.6, 2012, https://doi.org/10.7732/kjpr.2012.25.6.682
  2. Inhibitory Effect of Alpiniae officinarum Rhizoma Extract on Degranulation in RBL-2H3 Cells vol.28, pp.3, 2015, https://doi.org/10.7732/kjpr.2015.28.3.321
  3. Regulation of Immunological Effect of Rubia cordifolia Extract and Associated MAPKs Pathway in RBL-2H3 Cell-line vol.30, pp.6, 2012, https://doi.org/10.7732/kjpr.2017.30.6.662
  4. 해조류 유래 호흡기 질환 천식 치료제 연구 동향 vol.12, pp.1, 2020, https://doi.org/10.15433/ksmb.2020.12.1.001