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느티만가닥버섯의 신품종에 대한 endo-, exo-cellular 효소 활성도의 비교

Comparison of Endo-, Exo-Cellular Enzyme Activity for New Strains of Hypsizygus marmoreus

  • Lee, Chang-Yun (Greenpeace Mushroom Co.) ;
  • Song, Ho-Sung (Greenpeace Mushroom Co.) ;
  • Ro, Hyeon-Su (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University) ;
  • Woo, Ju-Ri (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • You, Young-Hyun (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Kim, Jong-Guk (Department of Life Sciences and Biotechnology, Kyungpook National University)
  • 투고 : 2012.04.11
  • 심사 : 2012.05.30
  • 발행 : 2012.06.30

초록

본 연구는 새롭게 개발된 느티만가닥버섯의 6개 품종에 대한 형태적, 생리적 특성을 조사하고 endo-, exo-cellular 효소 활성을 측정하기 위해서 수행되었다. 국내 야생종인 Hm3-10과 일본 재배종인 Hm1-1과의 단핵균사 교배를 통하여 343개의 교배 균주를 획득하여 재배를 실시하여 58개 균주를 1차 선발하고 2차로 6개 균주를 선발하였다. 6개 선발 균주를 대상으로 배양 일수 별로 재배를 실시한 결과 배양일수가 80일 이상에서는 재배일수가 19~20일로 단축되어 최적 배양일수를 80일로 결정하였다. 80일 배양일수에서 각 품종별 형태적 특성을 검증한 결과 Hm15-3, Hm15-4, Hm17-5의 3균주가 재배에 적합한 균주로 판명되었다. 각 균주의 endo-cellular 효소 활성도를 측정한 결과, ${\alpha}$-amylase의 효소 활성도가 73.9~102,2 unit/mg protein으로 가장 높았으며, chitinase 의 효소 활성도가 8.1~13.1 unit/mg protein으로 측정되었다. Exo-cellular효소 활성도를 측정한 결과, ${\alpha}$-amylase의 효소 활성도가 5,292~1,184 unit/mg protein으로 가장 높았으며, CMCase와 Xylanase의 효소 활성도가 각각 1,140~245 unit/mg protein, 94~575 unit/mg protein으로 측정되었다. 그러나 ${\beta}$-glucosidase와 chitinase의 활성도는 비교적 낮은 활성도를 나타내었다.

This study was carried out to investigate the morphological and physiological characteristics of six new cultivars of Hypsizygus marmoreus (Hm) and measure endo-, exo-cellular enzyme-specific activity. The domestic wild stain (Hm3-10) and commercial strain in Japan (Hm1-1) were mated by crossing monokaryon mycelia. We gained 58 strains from one of 400 crosses through the $1^{st}$ cultivation experiment, and selected six strains from one of 58 strains through the $2^{nd}$ cultivation experiment. When six of the selected new strains were grown during several spawn culture periods (60, 70, 80, 90, and 100 days), a spawn culture period of more 80 days was considered to be excellent as being shorter than 19~20 days. Therefore, we determined the period of spawn culture as 80 days. Three strains such as Hm15-3, Hm15-4, and Hm17-5 showed an excellent result. When endo-cellular enzyme activity measured eight strains, we obtained a result of that specific activity of ${\alpha}$-amylase at the highest as 73.9~102.2 unit/mg protein, and chitinase is lower than ${\alpha}$-amylase at 8.1~13.1 unit/mg protein. When exo-cellular enzyme activity measured eight strains, we determined the result of that specific activity of ${\alpha}$-amylase is the highest at 5,292~1,184 unit/mg protein, and CMCase and xylanase were 1,140~245 unit/mg protein, 94~575 unit/mg protein, compared to each other. However, the enzyme activity of ${\beta}$-glucosidase and chitinase is low.

키워드

참고문헌

  1. Bradford, M. M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  2. Chang, J. S., Son, J. K., Gao, L. and Oh, E. J. 2004. Inhibition of cell cycle progression on HepG2 cells by hypsiziprenol A9, isolated from Hypsizygus marmoreus. Cancer Lett. 212, 7-14. https://doi.org/10.1016/j.canlet.2004.03.013
  3. Chi, J. H., Park, W. K. and Kim, Y. H. 2000. Studies on improvement of cultural practice for Lyophllum ulmarium. Kor. J. Mycol. 28, 88-92.
  4. Danielsson, C. E. 1947. Molecular weight of alpha-amylase. Nature 160, 899.
  5. Harada, A., Gisusi, S., Yoneyama, S. and Aoyama, M. 2004. Effects of strain and cultivation medium on the chemical composition of the taste components in fruit-body of Hypsizygus marmoreus. Food Chem. 84, 265-270. https://doi.org/10.1016/S0308-8146(03)00210-3
  6. Hsu, S. C. and Lockwood, L. 1975. Powdered chitin agar as a selective medium for enumeration of Actinomycetes in water soil. Appl. Microbiol. 29, 422-426.
  7. Hyun, M. W., Yoon, J. H., Park, W. H. and Kim, S. H. 2006. Detection of cellulolytic activity in Ophiostoma and Leptographium species by chromogenic reaction. Microbiology 34, 108-110.
  8. Ikekawa, T., Saitoh, H., Feng, W., Zhang, H., Li, L. and Matsuzawa, T. 1992. Antitumor activity of Hypsizygus marmoreus. I. Antitumoractivity of extracts and polysaccharides. Chem. Pharm. Bull. 40, 1954-1957. https://doi.org/10.1248/cpb.40.1954
  9. Jeong, E. U. and Lee, Y. H. 1995. Isolation of microorganism producing chitinase for chito-oligosaccharides production, purification of chitinase, and its enzymatic characteristics. Kor. J. Microbiol. Biotechnol. 23, 187-196.
  10. Kanda, T., Wakabayashi, K. and Nisizawa, K. 1976. Purification and properties of an endo-cellulase of avicelase type from Irpx lacteus (Polyporus tuliferae). J. Ferment. Technol. 60, 381-383.
  11. Kim, D. J., Shin, H. J., Min, B. H. and Yoon, K. H. 1995. Isolation of a thermophilic Bacillus sp. producing the thermostable cellulose-free xylanase, and properties of the enzyme. Kor. J. Appl. Microbiol. Biotechnol. 23, 304-310.
  12. Kim, H. S., Ha, H. C. and Kim, T. S. 2003. Research and prospects in new functional mushroom-Tremella fuciformis, Grifora frondosa, and Hypsizygus marmoreus. Kor. J. Food Sci. Ind. 36, 42-46.
  13. Kwon, H. W., Back, J. B., Ko, H. G., You, C. H. and Kim, S. H. 2008. Extracellular enzyme activities of the monokaryotic strains generated from basidiospores of shiitake mushroom. Microbiology 36, 74-76.
  14. Lam, S. K. and Ng, T. B. 2001. Hypsin, a novel thermostable ribosome-inactivating protein with antifungal and antiproliferative activities from fruiting bodies of the edible mushroom Hypsizygus marmoreus. Biochem. Biophys. Res. Comm. 285, 1071-1075. https://doi.org/10.1006/bbrc.2001.5279
  15. Lee, C. Y., Hong, O. P., Jung, M. J. and Han, Y. H. 1998a. The extracellular enzyme activities in culture broth of Tricholoma matsutake. Kor. J. Mycol. 26, 496-501.
  16. Lee, C. Y., Park, J. E., Kim, B. B., Kim, S. M. and Ro, H. S. 2009b. Determination of mineral components in the cultivation substrates of edible mushrooms and their uptake into fruiting bodies. Microbiology 37, 109-113.
  17. Lee, C. Y., Park, J. E., Lee, J. A., Kim, J. K. and Ro, H. S. 2012. Development of new strains and related SCAR markers for and edible mushroom, Hypsizygus marmoreus. FEMS Microbiol Lett. 327, 54-59. https://doi.org/10.1111/j.1574-6968.2011.02458.x
  18. Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31, 426-428. https://doi.org/10.1021/ac60147a030
  19. Techapun, C., Poosaran, N., Waanabe, M. and Sasaki, K. 2003. Thermostable and alkaline-tolerant microbial celluase- free xylanase produced from agricultural wastes and the properties required for use in pulp bleaching bioprocesses: a review. Process Biochem. 38, 1327-1340. https://doi.org/10.1016/S0032-9592(02)00331-X
  20. Tokao, S., Kamagata, Y. and Sasaki. 1985. Cellulase production by Penicillium purogenum. J. Agri. Sci. Camb. 93. 217-222.
  21. Tsuchida, K., Aoyagi, Y., Odani, S., Mita, T. and Isemura, M. 1995. Isolation of a novel collagen-binding protein from the mushroom Hypsizygus marmoreus, which inhibits the Lewis lung carcinoma cell adhesion to type IV collagen. J. Biol. Chem. 270, 1481-1484. https://doi.org/10.1074/jbc.270.4.1481
  22. Xing, Z., Wang, Y., Feng, Z. and Liu, X. 2007. Effect of 60Co-irradiation on postharvest quality and selected enzyme activities of Hypsizygus marmoreus Fruit bodies. J. Agaric. Food Chem. 55, 8126-8132. https://doi.org/10.1021/jf070941w
  23. Xing, Z., Wang, Y., Feng, Z. and Tan, Q. 2008. Effect of different packaging films on postharvest quality and selected enzyme activities of Hypsizygus marmoreus mushrooms. J. Agaric. Food Chem. 56, 11838-11844. https://doi.org/10.1021/jf8024387
  24. Yoon, J. H., Hong, S. B., Ko, S. J. and Kim, S. H. 2007a. Detection of extracellular enzyme activity in Penicillium using chromogenic media. Microbiology 35, 166-169.
  25. Yoon, J. H., Park, J. E., Suh, D. Y., Hong, S. B., Ko, S. J. and Kim, S. H. 2007b. Comparison of dyes for easy detection of extracellular cellulases in fungi. Microbiology 35, 21-24.

피인용 문헌

  1. Mycelial viability and cultivation characteristics of Hypsizygus marmoreus after long-term storage in different conditions vol.12, pp.1, 2014, https://doi.org/10.14480/JM.2014.12.1.29