Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Reverse Effect of Salicylic Acid on Cd-induced Growth, Chlorophyll, and Rubisco/Rubisco Activase in Tobacco

카드뮴에 의해 유도된 담배 식물의 생장, 엽록소와 rubisco/rubisco activase에 대한 salicylic acid의 전환 효과

  • Received : 2012.03.15
  • Accepted : 2012.04.06
  • Published : 2012.06.30
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Abstract

The influence of salicylic acid (SA) on growth, chlorophyll, and rubisco/rubisco activase and effect of denaturator on rubisco/rubisco activase activity were studied in tobacco plants grown in vitro with cadmium (Cd) treatment. In order to find out the optimum concentration of SA, tobacco plants treated with $10^{-6}$ mM - $10^2$ mM of SA were grown in MS medium for 9 weeks, respectively. The most pronounced effect on in vitro growth was found at $10^{-4}$ mM SA. Among the control (not treated with Cd and SA), SA, Cd, and Cd + SA, the growth and content of chlorophyll were in the sequence of Cd < Cd + SA < control < SA, and significantly higher at SA compared with others. Similar results were also observed in the content and activity of rubisco and rubisco activase. These data suggest that inhibitory effect by Cd was reversed by SA. These results also indicate that SA has a positive effect on Cd. The effect of denaturants on rubisco activity showed in the sequence of Cd < Cd + SA < control < SA. Rubisco activity was promoted by L-cysteine and ${\beta}$-mercaptoethanol, not by urea, thiourea, and guanidium-HCl. These data suggest that urea, thiourea, and guanidium-HCl are able to act as denaturator, and L-cysteine and ${\beta}$-mercaptoethanol are not. None of the five denaturants affected the activity of rubisco activase.

카드뮴에 의해 유도되는 담배의 생장, 엽록소 함량, rubisco와 rubisco activase에 미치는 SA의 영향과, 이에 대한 변성제의 효과를 연구하였다. 담배 기내생장에 대한 SA의 최적농도를 찾기 위해, $10^{-6}$ mM - $10^2$ mM SA를 처리하여 9주간 생장시킨 결과, $10^{-4}$ mM SA에서 가장 높은 생장을 보였다. SA와 카드뮴을 4개의 실험구(대조구, SA, 카드뮴, 카드뮴 + SA)로 하여 생장, 엽록소 함량 및 rubisco와 rubisco activase의 함량과 활성을 측정한 결과, 카드뮴 > 카드뮴 + SA < 대조구 카드뮴 + SA < 대조구 ${\beta}$-mercaptoethanol은 활성을 촉진시켰으며, urea, thiourea, guanidium-HCl은 억제시켰다. 이는 L-cysteine과 ${\beta}$-mercaptoethanol은 변성에 관여하지 않으며, urea, thiourea, guanidium-HCl은 변성에 관여하였음을 의미한다. Rubisco activase의 활성에 대한 변성제들의 영향을 조사한 결과, 5종 모두는 비처리구와 비교하여 현저한 영향은 나타내지 않았다.

Keywords

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