Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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α-Glucosidase, Tyrosinase, and Elastase Inhibitory Effects of Enzymatic Extracts from Ecklonia cava and its Alcohol Metabolizing Activity

감태(E. cava Kjellman) 효소분해산물의 항당뇨 및 알코올 분해능과 미용효과

  • Kim, Hye-Youn (Department of Food and Nutrition, College of Medical Life Sciences, Silla University) ;
  • Cho, Eun-Kyung (Department of Food and Nutrition, College of Medical Life Sciences, Silla University) ;
  • Kang, Su-Hee (Department of Food and Nutrition, College of Medical Life Sciences, Silla University) ;
  • Bae, Jeong-Mi (Department of Food and Nutrition, College of Medical Life Sciences, Silla University) ;
  • Choi, Young-Ju (Department of Food and Nutrition, College of Medical Life Sciences, Silla University)
  • 김혜윤 (신라대학교 의생명과학대학 식품영양학과) ;
  • 조은경 (신라대학교 의생명과학대학 식품영양학과) ;
  • 강수희 (신라대학교 의생명과학대학 식품영양학과) ;
  • 배정미 (신라대학교 의생명과학대학 식품영양학과) ;
  • 최영주 (신라대학교 의생명과학대학 식품영양학과)
  • Received : 2012.02.27
  • Accepted : 2012.05.30
  • Published : 2012.06.30
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Abstract

Microbulbifer sp. was used to acquire the degrading products from Ecklonia cava (DPEC) and the products were investigated to determine the physiological activities. Firstly, 2,2-diphenyl-1-picrylhydrazyl (DPPH) activity and superoxide dismutase (SOD) assay were about 84.1% and 89.6% at 2.5 mg/ml, respectively. In addition, nitrite scavenging ability was shown to be 56.3% at 0.5 mg/ml on pH 1.2. ${\alpha}$-Glucosidase inhibitory activity was increased in a dose-dependent manner and was about 58.7% at 2.5 mg/ml. To determine the influence of DPEC on alcohol metabolism, the generating activity of reduced-nicotinamide adenine dinucleotide (NADH) by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were measured. Facilitating rates of ADH and ALDH activities by DPEC were 123.3% and 215.2% at 2.5 mg/ml, respectively. For analyses of anti-wrinkling and whitening effects, its elastase and tyrosinase inhibitory activities were measured and were about 73.1% and 42.2% at 2.5 mg/ml, respectively. These results indicated that DPEC has valuable biological attributes owing to its antioxidant, nitrite scavenging, and alcohol metabolizing activities and ${\alpha}$-glucosidase, elastase, and tyrosinase inhibitory activities.

본 연구에서는 기장 해안의 해수에서 분리한 해양 미생물로부터 해조류의 난소화성 복합다당류를 분해하는 균주를 분리하고 동정하여 Microbulbifer sp. SC092로 명명하였다. Microbulbifer sp. SC092 미생물에 의하여 생성된 조효소의 기질 특이성 분석으로 agar 및 alginic acid를 분해하는 효소활성이 각각 146.2%로 agarase와 alginase 효소활성이 높은 것으로 나타났다. 감태 효소분해산물의 항산화력은 DPPH radical 소거능과 SOD 활성으로 측정하였으며, DPPH radical 소거능과 SOD 활성은 각각 84.1, 89.6%로 나타났다. 감태 효소분해산물의 혈당 강하능은 ${\alpha}$-glucosidase 활성억제 효과를 측정하였으며 ${\alpha}$-glucosidase 활성은 58.7%로 나타났다. 감태 효소분해산물의 항염증 소재로써 이용 가능성은 인체의 위의 pH와 비슷한 조건에서 아질산염소거능 측정으로 확인하였는데, 0.5 mg/ml 농도에서 56.3%를 나타내어 positive control로 사용한 Vit C 보다 높은 활성을 보였다. 알코올분해능은 ADH와 ALDH 활성을 측정한 결과는 각각 123.3, 215.2%로 나타나 감태 효소분해산물의 우수한 숙취해소 효능을 보였다. 감태 효소분해산물의 미백 및 주름개선기능은 tyrosinase 및 elastase 저해효과로 분석하였다. 감태 효소분해산물의 농도가 증가함에 따라 미백 및 주름개선기능은 농도 의존적으로 증가하는 경향을 나타내었으며, 분해산물의 농도 2.5 mg/ml 농도에서 각각 42.2%와 73.1%로 높은 저해 효능을 나타내었다. 이러한 결과는 효소 추출법이 새로운 기능성 소재를 발굴하는데 효과적인 추출법인 것으로 기대된다.

Keywords

References

  1. Bae, J. M., Cho, E. K., Kim, H. Y., Kang, S. H. and Choi, Y. J. 2012. Biological analysis of enzymatic extracts from Capsosiphon fulvescens using marine bacterium Microbulbifer sp. AJ-3. J. Life Sci. 22, 627-633. https://doi.org/10.5352/JLS.2012.22.5.627
  2. Bergmeyer, H. U. 1974. Methods of enzymatic analysis. Academic Press, New York. pp. 28.
  3. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1198-1200.
  4. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  5. Bu, H. J., Ham, Y. M., Kim, J. M., Lee, S. J., Hyun, J. W. and Lee, N. H. 2006. Elastase and hyaluronidase inhibition activities of phlorotannins isolated from Ecklonia cava. Korean J. Pharmacogn. 37, 92-96.
  6. Cha, M. H. and Kim, Y. K. 2008. Analysis of consumption values of a seaweed functional food. Korean J. Food Culture 23, 462-468.
  7. Cho, E. K. and Choi, Y. J. 2010. Physiological activities of hot water extracts from Ecklonia cava Kjellman. J. Life Sci. 20, 1675-1682. https://doi.org/10.5352/JLS.2010.20.11.1675
  8. Dewitt, D. L., Rollins, T., Day, J. S., Gauger, J. A. and Smith, W. L. 1981. Orientation of the active site, and antigenic determinants of prostaglandin endoperoxide of synthase in the endoplasmic reticulum. J. Biol. Chem. 256, 10375-10382.
  9. Gray, J. I. and Dugan Jr, L. R. 1975. Inhibition of N-nitrosamine formation in model food systems. J. Food Sci. 40, 981-984. https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  10. Haug, A., Larsen, B. and Smidsrod, O. 1966. A study of constitution of alginic acid by partial acid hydrolysis. Acta. Chemica. Scand. 20, 183-190. https://doi.org/10.3891/acta.chem.scand.20-0183
  11. Heo, S. J. and Jeon, Y. J. 2005. Antioxidant effect and protecting effect against cell damage by enzymatic hydrolysates from marine algae. Food Ind. Nutr. 10, 31-41.
  12. Heu, M. S., Yoon, M. S., Kim, H. J., Park, K. H., Lee, J. H., Jo, M. R., Lee, J. S., Jeon, Y. J. and Kim, J. S. 2010. Improvement on the antioxidant activity of instant noodles containing enzymatic extracts from Ecklonia cava and its quality characterization. Kor. J. Fish Aquat. Sci. 43, 391-399.
  13. James, A. E. K., Timothy, D. W. and Gorden, L. 1996. Inhibition of human leujocyte and porcine pancreatic elastase by homologues of bovine pancreatic tyrosinase inhibitors. Biochem. 35, 9090-9096. https://doi.org/10.1021/bi953013b
  14. Jimenez-Escring, A. and Goni Cambrodon, I. 1999. Nutritional evaluation and physiological effects of edible seaweeds. Arch. Lationam. Nutr. 49, 114-120.
  15. Jonnadula, R. and Ghadi, S. C. 2011. Purification and characterization of agarase from seaweed decomposing bacterium Microbulbifer sp. strain CMC-5. Biotechnol. Bioproc. Eng. 16, 513-519. https://doi.org/10.1007/s12257-010-0399-y
  16. Joo, D. S., Lee, J. K., Choi, Y. S., Cho, S. Y., Je, Y. K. and Choi, J. W. 2003. Effect of sea tangle oligosaccharide drink on serum and hepatic lipids in rats fed a hyperlipidemic diet. J. Korean Soc. Food Sci. Nutr. 32, 1364-1369. https://doi.org/10.3746/jkfn.2003.32.8.1364
  17. Jung, E. Y. and Lee, S. K. 2007. Antioxidant activities and regeneration effect in HaCaT cell line by Jeju Island aboriginal Ecklonia cava. J. Korean Soc. Cosm. 13, 1071-1077.
  18. Kim, D.-H., Jung, J.-Y., Kim, K.-B.-W.-R., Lee, C.-J., Kwak, J.-H., Kim M.-J., Sun, W.-C., Kim, H.-J., Jung, S.-A., Kim, T.-W., Cho, Y.-J. and Ahn, D.-H. 2011. Effects of heat and pH treatments on $\alpha$-amylase inhibitory activity of Ecklonia cava ethanol extract. Kor. J. Fish Aquat. Sci. 44, 791-795.
  19. Kim, E., Kim, M.-S., Kim, S.-Y. and Kim, H.-A. 2008. Effect of Ecklonia cava on the blood glucose, lipids and renal oxidative stress in diabetic rats. Korean J. Food Culture 23, 812-819.
  20. Kim, J. A. and Lee, J. M. 2004. The changes of biologically functional compounds and antioxidant activities in Ecklonia cava with blanching times. Korean J. Food Culture 19, 369-377.
  21. Kim, J. W., Kim, D. K., Park, J. S., Lee, Y. K., Beik, K. Y. and Kim, S. D. 2009. Antioxidant and antimicrobial activities of shark collagens, and inhibitory actions on elastase and tyrosinase. Korean J. Food Preserv. 16, 419-426.
  22. Kim, M. K., Kim, J. Y., Choi, S. W., Hong, J. T. and Yoon, K. S. 2004. Anti-wrinkle effect of safflower (Cathamustinctorius) seed extract. J. Soc. Cosmet. Sci. Korea 30, 15-22.
  23. Koivula, T. and Koivusalo, M. 1975. Different from of rat liver aldehyde dehydrogenase and their subcellular distribution. Biochim. Biophys. Acta. 397, 9-23. https://doi.org/10.1016/0005-2744(75)90174-6
  24. Lee, H. O., Kim, D. S., Do, J. R. and Ko, Y. S. 1999. Angiotensin I converting enzyme inhibitory activity of algae. J. Korean Fish Soc. 32, 427-431.
  25. Lee, S. H., Kim, K. N., Cha, S. H., Ahn, G. N. and Jeon, Y. J. 2006. Comparison of antioxidant activities of enzymatic and methanolic extracts from Ecklonia cava stem and leave. J. Korean Soc. Food Sci. Nutr. 35, 1139-1145. https://doi.org/10.3746/jkfn.2006.35.9.1139
  26. Leelarungrayub, N., Rattanapanone, V., Chanarat, N. and Gebicki, J. M. 2006. Quantitative evaluation of the antioxidant properties of garlic and shallot preparations. Nutrition 22, 266-274. https://doi.org/10.1016/j.nut.2005.05.010
  27. Miller, G. L. 1959. Use of dinitrosalicylic acid regent for determination of reducing sugar. Anal. Chem. 31, 426. https://doi.org/10.1021/ac60147a030
  28. Park, Y. B., Lee, T. G., Kim, O. K., Do, J. R., Yeo, S. G., Park, Y. H. and Kim, S. B. 1995. Characteristics of nitrite scavenger derived from seeds of Cassia tora L. Korea J. Food Sci. Technol. 27, 124-128.
  29. Peters, T. J. 1982. Ethanol metabolism. Bri. Med. Bull. 38, 17-20.
  30. Shinde, J., Taldone, T., Barletta, M., Kunaparaju, N., Hu, B., Kumer, S., Placido, J. and William, Z. S. 2008. $\alpha$-Glucosidase inhibitory activity of Syzygium cumini (Linn). Skeels seed kernel in vitro and in Goto-Kakizaki (GK) rats. Carbohydrate Res. 343, 1278-1281. https://doi.org/10.1016/j.carres.2008.03.003
  31. Sies, H. 1999. Glutathione and its role in cellular functions. Free Radical Bio. Med. 27, 916-921. https://doi.org/10.1016/S0891-5849(99)00177-X
  32. Song, H. J. 2009. Molecular cloning and characterization of agarase gene from marine bacterium, Microbulbifer sp. AJ-3. MS Thesis. Silla University.
  33. Tibbot, B. K. and Skadsen, R. W. 1996. Molecular cloning and characterization of a gibberellin-inducible, putative $\alpha$-glucosidase gene from berley. Plant Mol. Biol. 30, 229-241. https://doi.org/10.1007/BF00020110
  34. Wiseman, H. 1996. Dietary influences on membrane function; important in protection against oxidative damage and disease. J. Nutri. Biochem. 7, 2-6. https://doi.org/10.1016/0955-2863(95)00152-2
  35. Yagi, A., Kanbara, T. and Morinobu, N. 1987. Inhibition of mushroom tyrosinase by aloe extract. Planta Medica. 53, 517-519. https://doi.org/10.1055/s-2006-962799

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