Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Evaluation of Cytotoxic Properties of Caffeine Treated with Over-the-counter Drugs in the Intestinal Cells

카페인과 일반의약품의 복합처리에 의한 장관계 세포 독성 평가

  • Choi, Hyun-A (Department of Food Science and Technology, College of Natural Science, Seoul Women’s University) ;
  • Kim, Mi-Ri (Department of Food Science and Technology, College of Natural Science, Seoul Women’s University) ;
  • Park, Kyung-A (Department of Food Science and Technology, College of Natural Science, Seoul Women’s University) ;
  • Hong, Jung-Il (Department of Food Science and Technology, College of Natural Science, Seoul Women’s University)
  • 최현아 (서울여자대학교 자연과학대학 식품공학과) ;
  • 김미리 (서울여자대학교 자연과학대학 식품공학과) ;
  • 박경아 (서울여자대학교 자연과학대학 식품공학과) ;
  • 홍정일 (서울여자대학교 자연과학대학 식품공학과)
  • Received : 2012.02.09
  • Accepted : 2012.03.06
  • Published : 2012.06.30
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Abstract

Caffeine is a xanthine alkaloid derivative found in many foods and beverages. Dietary caffeine may interact with commonly-consumed over-the-counter (OTC) drugs in body. In this study, cytotoxic effects on the intestinal cells by combined treatment of caffeine with several OTC drugs, including ibuprofen, aspirin, and acetaminophen. Cytotoxic effect of caffeine was more potent in normal intestinal INT 407 cells than in colon cancer HCT 116 cells. Relative toxicity of caffeine and the OTC drugs was significantly enhanced in INT 407 cells when treated together. Intracellular thiol levels of the cells treated with the OTC drugs increased in the presence of caffeine. When HCT 116 cells were incubated with each OTC drug after or before caffeine treatment, the relative cytotoxicity of the OTC drugs increased. The present study may provide basic information about possible health effects through the interactions between caffeine and OTC drugs in the intestinal cells.

다양한 식품 중에 널리 분포되어 있는 생리활성 성분 카페인과 일반의약품 성분 AAP, Asp 및 Ibu와의 혼용 시 상호작용에 의한 세포 독성 변화를 장관계 세포모델에서 조사하였다. 카페인은 정상 장관계 세포 INT 407 및 대장암 세포 HCT 116에 농도의존적인 독성을 나타내었고, $IC_{50}$ 수치는 각각 1.91과 2.45 mM로써 정상세포에 유의적으로 높은 독성을 나타내었다. 카페인과 각각의 약물을 세포에 24시간 동시 처리한 결과 전체적으로 현저한 독성의 변화현상은 발생하지 않았으나, 약물처리 시를 기준으로 한 카페인의 상대적 독성 및 카페인 처리 시를 기준으로 한 약물의 독성이 INT 407 세포에서 유의적으로 증가하였다. 동시 처리 시 약물에 의해 감소된 GSH를 비롯한 thiol성 물질의 세포 내 수준이 카페인 존재 시에 유의적으로 증가하였다. 한편 카페인과 약물을 각각 순서를 달리하여 전후로 처리하였을 때, 특히 HCT 116 세포에서의 약물의 상대적인 독성이 강화되는 현상을 보였다. 일반의약품 AAP, Asp 및 Ibu과 카페인을 다양한 조합에 의해 장관계 세포에 처리하였을 때 일부 상대적인 독성의 강화 또는 약화 현상이 나타났으나 전체적으로 두드러진 독성발현 빛 활성변화는 발견되지 않았다.

Keywords

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