한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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목이버섯 품종간 생리활성 비교 연구

A Comparative Study on the Physiological Activities of Auricularia spp.

  • 조세현 (성균관대학교 식품생명공학과) ;
  • 김태호 (성균관대학교 식품생명공학과) ;
  • 유영복 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 오진아 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 장미향 (전남농업기술원) ;
  • 박기문 (성균관대학교 식품생명공학과)
  • Jo, Se-Hyun (Department of Food Science and Biotechnology, Sungkyunkwan University) ;
  • Kim, Tae-Ho (Department of Food Science and Biotechnology, Sungkyunkwan University) ;
  • Yu, Young-Bok (Mushroom research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Oh, Jin-A (Mushroom research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jang, Mi-Hyang (Jeonnam Agricultural Research & Extension Services) ;
  • Park, Ki-Moon (Department of Food Science and Biotechnology, Sungkyunkwan University)
  • 투고 : 2012.02.08
  • 심사 : 2012.03.20
  • 발행 : 2012.06.30
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초록

본 연구에서는 목이버섯 품종간의 생리활성을 실험하였다. ORAC assay로 항산화활성을 측정한 결과 털목이, 갈색목이, 흑목이 순으로 활성이 높게 나타났다. ${\alpha}$-Amyloglucosidase 저해활성을 통한 항당뇨 활성은 3품종 모두 10 mg/mL에서 60% 정도의 높은 저해활성을 나타냈으며 품종간 유의적인 차이는 나타나지 않았다. Raw 264.7 macrophage의 nitric oxide 생성 저해를 통한 항염활성은 1 mg/mL 처리시 털목이와 흑목이는 dexamethasone 1 ${\mu}g$/mL에 상응하는 활성을 나타냈다. 20% 지방을 첨가하여 고지방식이로 비만을 유도한 ICR 마우스의 목이버섯 급여에 따른 항비만 효과를 확인한 결과 모든 군에서 체중 및 증체량, 식이효율의 감소를 확인할 수 있었고 그 중 갈색목이버섯인 JNM21012군은 0.45 g/day로 7% 지방을 첨가한 일반식이(NC) 군과 동일 수준의 증체량을 보였다. 부고환 무게는 털목이 및 흑목이, 갈색목이 모두 고지방식이(HFC) 군에 비해서 낮은 수치를 보였으며, 갈색목이 군은 HFC군과 유의적인 차이를 보였다. 혈액성분 중 triglyceride의 경우 목이버섯 첨가 식이군 모두 HFC군에 비해 낮은 수치를 보였으며 갈색목이 군은 고지방식이 HFC군에 비해 42% 감소시키는 것으로 나타났다. 또한, total cholesterol과 LDL-cholesterol은 털목이와 갈색목이군이 HFC군 보다 낮은 수치를 나타냈으며, 갈색목이군은 HFC군에 비해 각각 81%와 66% 감소하여 유의적인 차이를 보였다. HDL-cholestrol의 경우 각 군간의 유의적인 차이를 보이지 않았지만 NC군에 비해 높은 수치를 나타냈다.

This study investigated the physiological effects of three species of Auricularia known as Auricularia polytricha (JNM21001), Auricularia auricula-judae black (JNM21002), and Auricularia auricula-judae brown (JNM21012). In the ORAC assay, Auricularia spp. showed antioxidant activities in the order of JNM21001>JNM21012>JNM21002. All Auricularia spp. strongly inhibited the action of ${\alpha}$-amyloglucosidase up to 60%. In order to further test in vivo anti-obesity effects, high fat diet induced ICR obese mice fed a diet containing 20% fat were used. All Auricularia spp. supplementation during high fat diet feeding significantly reduced body weight gain, epididymal fat pads weight, and lowered the food efficiency ratio compared to the high fat control (HFC) group. In particular, the group fed with JNM21012 had a lower average daily body weight gain of 0.45 g/day, demonstrating similar levels to the normal diet fed group. The group fed with JNM21012 significantly reduced lowered serum triglycerides (42%), total cholesterol (81%), and LDL-cholesterol level (66%) compared with the HFC group.

키워드

참고문헌

  1. Zhang L, Yang L, Chen J. Conformational change of the $\beta$-Dglucan of Auricularia auricula-judae in water dimethyl sulfoxide mixtures. Carbohydr. Res. 276: 443-447 (1995) https://doi.org/10.1016/0008-6215(95)00185-V
  2. Mattila PH, Piironen VI, Uusi Rauva EJ, Koivistoinen PE. Vitamin D contents in edible mushrooms. J. Agr. Food Chem. 42: 2449-2453 (1994) https://doi.org/10.1021/jf00047a016
  3. Roland JF, Chmielewicz ZF, Weiner BA, Gross AM, Boening OP, Luck JV, Stevens JA. Calvacin: A new antitumor agent. Science 132: 1897 (1960) https://doi.org/10.1126/science.132.3443.1897
  4. Kabir YK, Kimura S, Tamura T. Dietary effect of Ganoderma lcidum mushroom on blood pressure and lipid levels in spontanously hypertensive rats (SHR). J. Nutr. Sci. Vitaminol. 34: 433-438 (1998)
  5. Kiho T, Tsujimura Y, Sakushima M, Usui S, Ukai S. Polysaccharides in fungi. XXXIII. Hypoglycemic activity of an acidic polysaccharide (AC) from Tremella fuciformis. Yakugaku Zasshi 114: 308-315 (1994) https://doi.org/10.1248/yakushi1947.114.5_308
  6. Muller WEG, Weiler BE, Charubala R, Pfleiderer W, Leserman L, Sobol RW, Suhadolnik RJ, Schroder HC. Cordycepin analogues of 2',5'-oligoadenylate inhibit human immunodeficiency virus infection via inhibition of reverse transcriptase. Biochemistry 30: 2027-2033 (1991) https://doi.org/10.1021/bi00222a004
  7. Erkel G, Anke T, Gimenez A, Steglich W. Antibiotics from basidiomycetes. XLI. Clavicoronic acid, a novel inhibitor of reverse transcriptases from Clavicorona pyxidata (Pers. ex Fr.) Doty. J. Antibiot. 45: 29-37 (1992) https://doi.org/10.7164/antibiotics.45.29
  8. Maeda YY, Chihara G. Lentinan, a new immuno-accelerator of cell-mediated responses. Nature 229: 634 (1971) https://doi.org/10.1038/229634a0
  9. Chen FF, Cai DL. Research advances in primary biological effects of Tremella polysaccharides. J. Chin. Integr. Med. 6: 862-866 (2008) https://doi.org/10.3736/jcim20080818
  10. Kho YS, Vikineswary S, Abdullah N, Kuppusamy UR, Oh HI. Antioxidant capacity of fresh and processed fruit bodies and mycelium of Auricularia auricular-judae (Fr.) quel. J. Med. Food 12: 167-174 (2009) https://doi.org/10.1089/jmf.2007.0568
  11. Ma Z, Wang J, Zhang L. Structure and chain conformation of $\beta$-glucan isolated from Auricularia auricular judae. Biopolymers 89: 614-622 (2008) https://doi.org/10.1002/bip.20971
  12. Li S, Xu S, Zhang L. Advances in conformations and characterizations of fungi polysaccharides. Acta Polym. Sin. 12: 1359-1375 (2010)
  13. Tang L, Xiao Y, Li L, Guo Q, Bian Y. Analysis of genetic diversity among Chinese Auricularia auricula cultivars using combined ISSR and SRAP markers. Curr. Microbiol. 61: 132-140 (2010) https://doi.org/10.1007/s00284-010-9587-4
  14. Reeves PG, Nielsen FH, Fahey GC Jr. AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J. Nutr. 123: 1939-1951 (1993) https://doi.org/10.1093/jn/123.11.1939
  15. Huang D, Ou B, Prior RL. Reviews, The chemistry behind antioxidant capacity assays. J. Agr. Food Chem. 53: 1841-1856 (2005) https://doi.org/10.1021/jf030723c
  16. Prior RL, Hoang H, Gu L, Wu X, Bacchiocca M, Howard L, Hampsch-Woodill M, Huang D, Ou B, Jacob R. Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (ORAC)) of plasma and other biological and food samples. J. Agr. Food Chem. 51: 3273-3279 (2003) https://doi.org/10.1021/jf0262256
  17. Dubost NJ, Ou B, Beelman RB. Quantification of polyphenols and ergothioneine in cultivated mushrooms and correlation to total antioxidant capacity. Food Chem. 105: 727-735 (2007) https://doi.org/10.1016/j.foodchem.2007.01.030
  18. Um SN, Jin GE, Park KW, Yu YB, Park KM. Physiological activity and nutritional composition of Pleurotus species. Korean J. Food Sci. Technol. 42: 90-96 (2010)
  19. Kim HK, Yang ES, Park KM, Kim GH, Kim HH, Lee KS. Chemical components, antioxidant activity, and $\alpha$-glucoamylase inhibitory activity of a new mushroom variety 'Dahyang'. J. Korean Soc. Food Sci. Nutr. 40: 1179-1183 (2011) https://doi.org/10.3746/jkfn.2011.40.8.1179
  20. Higuchi M, Hisgahi N, Taki H, Osawa T. Cytolytic mechanisms of activated macrophages. Tumor necrosis factor and L-argininedependent mechanisms act synergistically as the major cytolytic mechanisms of activated macrophages. J. Immunol. 144: 1425-1431(1990)
  21. Moro C, Palacios I, Lozano M, D'Arrigo M, Guillamon E, Villares A, Martínez JA, Garcia-Lafuente A. Anti-inflammatory activity of methanolic extracts from edible mushrooms in LPS activated Raw 264.7 macrophages. Food Chem. 130: 350-355 (2012) https://doi.org/10.1016/j.foodchem.2011.07.049
  22. Bourdon I, Yokoyama W, Davis P, Rabert R, Richter D, Knuckles B, Schneernan BO. Postprandial lipid, glucose, insulin, and cholecystokinin responses in men fed barley pasta enriched with $\beta$-glucan. Am. J. Clin. Nutr. 69: 55-63 (1999) https://doi.org/10.1093/ajcn/69.1.55
  23. Nicolosi R, Bell SJ, Bistrian BR, Greenberg I, Forse RA, Blackburn GL. Plasma lipid changes after supplementation with $\beta$-glucan fiber from yeast. Am. J. Clin. Nutr. 70: 208-212 (1999) https://doi.org/10.1093/ajcn.70.2.208
  24. Chandra RK. Cell-mediated immunity in genetically obese mice. Am J. Clin. Nutr. 33: 13-19 (1980) https://doi.org/10.1093/ajcn/33.1.13
  25. Parrish CC, Pathy DA, Parkes JE, Angel A. Dietary fish oils modify adipocytes structure and function. J. Cell. Physiol. 148: 493-502 (1991) https://doi.org/10.1002/jcp.1041480323
  26. Yuan Z, He P, Cui J, Takeuchi H. Hypoglycemic effect of watersoluble polysaccharide from Auricularia auricular judae Quel. on genetically diabetic KK-Ay mice. Biosci. Biotech. Bioch. 62: 1898-1903 (1998) https://doi.org/10.1271/bbb.62.1898
  27. Goldberg IJ. Lipoprotein lipase and lipolysis: Central roles in lipoprotein metabolism and atherogenesis. J. Lipid Res. 37: 693-707 (1996)
  28. Assmann G, Schulte H . The prospective cardiovascular munster (PROCAM) study: Prevalence of hyperlipidemia in persons with hypertension and/or diabetes mellitus and the relationship to coronary heart disease. Am. Heart J. 116: 1713-1724 (1988) https://doi.org/10.1016/0002-8703(88)90220-7
  29. Kim GJ, Kim HS, Chung SY. Effects of varied mushroom on lipid compositions in dietary hypercholesterolemic rats. J. Korean Soc. Food Nutr. 21: 131-135 (1992)
  30. Blair SN, Kohl III HW, Paffenbarger RS Jr, Clark DG, Cooper KH, Gibbons LW. Physical fitness and all-cause mortality: A prospective study of healthy men and women. J. Am. Med. Assoc. 262: 2395-2401 (1989) https://doi.org/10.1001/jama.1989.03430170057028
  31. Smutok MA, Reece C, Kokkinos PF, Farmer C, Dawson P, Shulman R, DeVane-Bell J, Patterson J, Charabogos C, Goldberg AP, Hurley BF. Aerobic versus strength training for risk factor intervention in middle-aged men at high risk for coronary heart disease. Metabolism 42: 177-184 (1993) https://doi.org/10.1016/0026-0495(93)90032-J

피인용 문헌

  1. Antioxidant Activities and Antimicrobial Effects of Extracts from Auricularia auricula-judae vol.45, pp.3, 2016, https://doi.org/10.3746/jkfn.2016.45.3.327
  2. Comparison of Antioxidant Activities of Pileus and Stipe from White Beech Mushrooms (Hypsizygus marmoreus) vol.26, pp.8, 2016, https://doi.org/10.5352/JLS.2016.26.8.928