Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Anti-inflammatory effects of HC001 on atopic dermatitis-like skin lesions in mice

HC001의 아토피성 피부염에 대한 항염증 효능 및 기전 연구

  • Choi, You-Youn (Dept. of Physiology, College of Oriental Medicine, Kyung-Hee University) ;
  • Kim, Mi-Hye (Dept. of Physiology, College of Oriental Medicine, Kyung-Hee University) ;
  • Kum, Chang-Jun (Dept. of Anatomy, College of Oriental Medicine, Kyung-Hee University) ;
  • Choi, Young-Jin (Dept. of Anatomy, College of Oriental Medicine, Kyung-Hee University) ;
  • Hwang, Man-Ki (Dept. of Anatomy, College of Oriental Medicine, Kyung-Hee University) ;
  • Sohn, Young-Joo (Dept. of Anatomy, College of Oriental Medicine, Kyung-Hee University) ;
  • Jung, Hyuk-Sang (Dept. of Anatomy, College of Oriental Medicine, Kyung-Hee University) ;
  • Yang, Woong-Mo (Dept. of Physiology, College of Oriental Medicine, Kyung-Hee University)
  • 최유연 (경희대학교 한의과대학 처방제형학교실) ;
  • 김미혜 (경희대학교 한의과대학 처방제형학교실) ;
  • 금창준 (경희대학교 한의과대학 해부학교실) ;
  • 최영진 (경희대학교 한의과대학 해부학교실) ;
  • 황만기 (경희대학교 한의과대학 해부학교실) ;
  • 손영주 (경희대학교 한의과대학 해부학교실) ;
  • 정혁상 (경희대학교 한의과대학 해부학교실) ;
  • 양웅모 (경희대학교 한의과대학 처방제형학교실)
  • Received : 2012.05.21
  • Accepted : 2012.06.11
  • Published : 2012.06.30
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Abstract

Objectives : The purpose of this study is to examine the effects of external application of HC001 on atopic dermatitis. HC001, newly composed herbal medicine combinations contains 9 medicinal herbs which are known to have anti-inflammatory and anti-allergic effects. We investigated the anti-inflammatory effects of HC001 on atopic dermatitis-like skin lesions in mice. Methods : Seven-week-old BALB/c mice were sensitized with DNCB to develop atopic dermatitis. Animals were divide into three group: Normal, DNCB (Negative control group), HC001 (Experimental group, treated with DNCB and HC001). Skin sections were stained with H&E to measure the thicknesses of the epidermis and dermis, respectively. The expression of NF-${\kappa}B$ protein was measured by western blotting analysis in skin lesion. Results : Topical HC001 treatment significantly restored the skin thickening and hyperplasia of the epidermis and dermis compared with DNCB-sensitized group in histopathological analyse. In addition, HC001 inhibited the expression of NF-${\kappa}B$ protein increased in DNCB-induced atopic dermatitis-like skin lesions in mice. Conclusions : These results suggest that HC001 may be useful as an external application agent for atopic dermatitis based on reductions of various inflammatory responses.

본 실험에서는 피부질환을 위한 새로 구성된 HC001의 처방이 외용제로서 아토피성 피부질환에서의 항염증 효능을 확인하였다. H&E염색을 통한 조직학적 검사에서 DNCB로 유도된 진피 및 표피의 과각화증이 HC001 국소도포로 인해 호전되는 것을 관찰되었으며, 염증과정에서 중요한 지표인 NF-${\kappa}B$의 활성이 억제됨을 확인하였다. 이를 통해 HC001이 아토피성 피부질환의 외용제 사용에 있어 일정한 효과를 나타낼 수 있을 것으로 기대 된다.

Keywords

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