Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Preparation of Chitooligosaccharides from Chitosan using Crude Enzyme of Bacillus cereus D-11

  • Gao, Xing-Ai (Institute of Rural Energy, Jilin Academy of Agricultural Sciences) ;
  • Zhang, Yong-Feng (Institute of Rural Energy, Jilin Academy of Agricultural Sciences) ;
  • Park, Ro-Dong (Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Huang, Xiao (Institute of Rural Energy, Jilin Academy of Agricultural Sciences) ;
  • Zhao, Xin Ying (Institute of Rural Energy, Jilin Academy of Agricultural Sciences) ;
  • Xie, Jiao (Institute of Rural Energy, Jilin Academy of Agricultural Sciences) ;
  • Jin, Rong-De (Agricultural Environment and Resources Research Center, Jilin Academy of Agricultural Sciences)
  • Received : 2011.12.28
  • Accepted : 2012.02.01
  • Published : 2012.03.31
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Abstract

In order to enzymatically produce chitooligosaccharide using the crude enzyme preparation from Bacillus cereus D-11, we first studied the optimal reaction conditions. It was found that the optimal temperature for hydrolysis of chitosan was $55^{\circ}C$. The ratio of enzyme/substrate should not be lower than 0.13 U/mg in the reaction mixture. The enzyme activity was stable below $50^{\circ}C$. The products of enzymatic reaction were analyzed by both thin layer chromatography and high performance liquid chromatography. Under the appropriate condition, chitosan was hydrolyzed using the enzyme preparation. The resulting chitooligosaccharides were purified and separated by Dowex ($H^+$) ion exchange chromatography. From 4 g soluble chitosan, 0.95 g $(GlcN)_2$, 1.43 g $(GlcN)_3$, and 1.18 g $(GlcN)_4$ were recovered.

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References

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