Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Effect of Mature Miscanthus sacchariflorus var. No. 1 on In Vitro Rumen Fermentation Characteristics and Its Dry Matter Digestibility

생육 후기 거대억새의 In vitro 반추위 발효특성 및 건물 소화율

  • Cho, Sang-Buem (Department of Animal Science, Chonbuk National University) ;
  • Mbiriri, David Tinotenda (Department of Animal Science, Chonbuk National University) ;
  • Oh, Sung-Jin (Department of Animal Science, Chonbuk National University) ;
  • Lee, A-Reum (Department of Animal Science, Chonbuk National University) ;
  • Yang, Jin-Ho (Department of Animal Science, Chonbuk National University) ;
  • Ryu, Chae-Hwa (Department of Animal Science, Chonbuk National University) ;
  • Park, Chang-Min (Department of Animal Science, Chonbuk National University) ;
  • Moon, Yun-Ho (Bioenergy Crop Research Center, National Institute of Crop Science, RDA) ;
  • Chae, Jung-Il (Department of Oral Pharmacology, School of Dentistry and Institute of Dental Bioscience, Chonbuk National University) ;
  • Choi, Nag-Jin (Department of Animal Science, Chonbuk National University)
  • Received : 2012.05.31
  • Accepted : 2012.06.18
  • Published : 2012.06.30
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Abstract

This study was conducted to develop Miscanthus as a new roughage resource for ruminant animals. Miscanthus sacchariflorus var. No 1, a newly developed germtype in Korea, was harvested at late maturity stage and its effect on rumen pH, ammonia nitrogen, gas production, volatile fatty acid (VFA) production and digestibility were evaluated using in vitro rumen fermentation. The effects of Miscanthus were compared with rice straw. Miscanthus showed significantly higher pH compared to rice straw (p<0.01). As for ammonia nitrogen, there was no significant difference after 12 h of incubation (p>0.05). Gas production in Miscanthus was significantly lower than that of rice straw in overall incubation time (p<0.05) after 6 h of incubation. In VFA production, acetate, propionate, butyrate, valerate and total VFA production in Miscanthus were lower than those in rice straw. However, production of iso-butyrate and iso-valerate were not different in between two forage materials. Dry matter digestibility of Miscanthus was significantly lower than rice straw (p<0.05) during 12~24 h of incubation. As a result, the availability of Miscanthus as roughage source showed approximately 80% that of rice straw.

본 연구는 반추동물의 조사료 자원으로서 거대억새를 개발하기 위한 목적으로 수행되었다. 우리나라에서 새롭게 개발된 품종인 거대억새 1호를 완숙기 이후에 채취하여 in vitro 반추위 발효를 이용해 반추위내 pH, 암모니아태 질소, 가스발생량, 휘발성 지방산 생성량 및 건물소화율을 조사하였으며, 볏짚과 비교하여 평가하였다. 거대억새는 볏짚에 비하여 유의적으로 높은 반추위내 pH를 나타내었다(p<0.01). 암모니아태 질소의 경우 배양 12시간 이후에는 두 처리구간의 유의적인 차이를 나타내지 않았다 (p>0.05). 배양 6시간 이후 부터는 거대억새의 가스발생량이 볏짚에 비하여 유의적으로 낮게 나타났다 (p<0.05). 휘발성 지방산 생성량에 있어 acetate, propionate, butyrate, valerate 및 총생상량에서 볏짚이 거대억새보다 높게 나타났다. 그러나 iso-butyrate와 iso-valerate에서는 두 조사료원별 차이는 발견되지 않았다. 건물소화율에 있어 배양 12~24시간 사이의 거대억새 소화율이 볏짚에 비하여 유의적으로 나타났다. 결론적으로 거대억새의 이용성은 볏짚의 약 80% 수준인 것으로 나타났다.

Keywords

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