원예과학기술지 (Horticultural Science & Technology)

  • 제30권3호
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  • Pages.243-249
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  • 2012
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  • 1226-8763(pISSN)
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  • 2465-8588(eISSN)
한국원예학회 (Korean Society of Horticultural Science)
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Isolation and Characterization of a Gene Encoding Hexokinase from Loquat (Eriobotrya japonica Lindl.)

  • Qin, Qiaoping (School of Agriculture and Food Science, Zhejiang Agriculture and Forestry University) ;
  • Zhang, Lanlan (School of Agriculture and Food Science, Zhejiang Agriculture and Forestry University) ;
  • Xu, Kai (School of Agriculture and Food Science, Zhejiang Agriculture and Forestry University) ;
  • Jiang, Li (School of Agriculture and Food Science, Zhejiang Agriculture and Forestry University) ;
  • Cheng, Longjun (The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture & Forestry University) ;
  • Xu, Chuanmei (The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture & Forestry University) ;
  • Cui, Yongyi (School of Agriculture and Food Science, Zhejiang Agriculture and Forestry University)
  • 투고 : 2012.02.07
  • 심사 : 2012.03.13
  • 발행 : 2012.06.30
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초록

Hexokinase is the first enzyme in the hexose assimilation pathway; it acts as a sensor for plant sugar responses, and it is also important in determining the fruit sugar levels. The full-length cDNA of a hexokinase gene was isolated from loquat through reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends, which was designated as EjHXK1. EjHXK1 is 1,839 bp long and contains an entire open reading frame encoding 497 amino acids. The predicted protein of EjHXK1 shares 72%-81% similarity with other plant hexokinases. Phylogeny analysis indicated that EjHXK1 is closely related to maize and rice hexokinases. Transient expression of the 35S: EjHXK1-GFP fusion protein was observed on the cell membrane and cytoplasm. Real-time RT-PCR indicated that EjHXK1 is expressed in loquat leaves, stems, flowers, and fruits. EjHXK1 transcripts were higher during early fruit development, but decreases before maturation, which is consistent with hexokinase enzyme activity during fruit development and conducive for hexose accumulation in mature fruits. These results imply that EjHXK1 may play important roles in the regulation of sugar flux during fruit ripening.

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피인용 문헌

  1. Isolation and Characterization of A Cytosolic Pyruvate Kinase cDNA From Loquat (Eriobotrya japonica Lindl.) vol.31, pp.1, 2013, https://doi.org/10.1007/s11105-012-0479-6
  2. Isolation and induced expression of a fructokinase gene from loquat vol.61, pp.3, 2014, https://doi.org/10.1134/S1021443714030121
  3. Overexpression of a loquat (Eriobotrya japonica Lindl.) vacuolar invertase affects sucrose levels and growth vol.123, pp.1, 2015, https://doi.org/10.1007/s11240-015-0817-0
  4. Characterization of SWEET family members from loquat and their responses to exogenous induction vol.13, pp.6, 2017, https://doi.org/10.1007/s11295-017-1200-6
  5. Characterisation of the subunit genes of pyrophosphate-dependent phosphofructokinase from loquat (Eriobotrya japonica Lindl.) vol.10, pp.5, 2012, https://doi.org/10.1007/s11295-014-0774-5