Journal of Ginseng Research

  • 제36권3호
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  • Pages.242-247
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  • 2012
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  • 1226-8453(pISSN)
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  • 2093-4947(eISSN)
고려인삼학회 (The Korean Society of Ginseng)
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Anti-apoptotic Activity of Ginsenoside Rb1 in Hydrogen Peroxide-treated Chondrocytes: Stabilization of Mitochondria and the Inhibition of Caspase-3

  • Na, Ji-Young (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Sok-Ho (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Song, Ki-Bbeum (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Lim, Kyu-Hee (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Shin, Gee-Wook (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Jong-Hoon (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Bum-Seok (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Kwon, Young-Bae (Department of Pharmacology, School of Medicine, Chonbuk National University) ;
  • Kwon, Jung-Kee (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University)
  • 투고 : 2011.11.09
  • 심사 : 2012.02.10
  • 발행 : 2012.07.15
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초록

Chondrocyte apoptosis has been recognized as an important factor in the pathogenesis of osteoarthritis (OA). Hydrogen peroxide ($H_2O_2$), which produces reactive oxygen species, reportedly induces apoptosis in chondrocytes. The ginsenoside $Rb_1$ (G-$Rb_1$) is the principal component in ginseng and has been shown to have a variety of biological activities, such as anti-arthritis, anti-inflammation, and anti-tumor activities. In this study, we evaluated the effects of G-$Rb_1$ on the mitochondrial permeability transition (MPT) and caspase-3 activity of chondrocyte apoptosis induced by $H_2O_2$. Cultured rat articular chondrocytes were exposed to $H_2O_2$ with or without G-$Rb_1$ and assessed for viability, MPT, Bcl-xL/Bax expression, caspase-3 activity, and apoptosis. The co-treatment with G-$Rb_1$ showed an inhibition of MPT, caspase-3 activity, and cell death. Additionally, the levels of the apoptotic protein Bax were significantly lower and the levels of the anti-apoptotic protein Bcl-xL were higher compared with $H_2O_2$ treatment alone. The results of this study demonstrate that G-$Rb_1$ protects chondrocytes against $H_2O_2$-induced apoptosis, at least in part via the inhibition of MPT and caspase-3 activity. These results demonstrate that G-$Rb_1$ is a potentially useful drug for the treatment of OA patients.

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참고문헌

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  14. Mitochondrial connection to ginsenosides vol.43, pp.10, 2012, https://doi.org/10.1007/s12272-020-01279-2
  15. Emerging Natural-Product-Based Treatments for the Management of Osteoarthritis vol.10, pp.2, 2012, https://doi.org/10.3390/antiox10020265
  16. Review of ginsenosides targeting mitochondrial function to treat multiple disorders: Current status and perspectives vol.45, pp.3, 2021, https://doi.org/10.1016/j.jgr.2020.12.004
  17. Ginsenoside Rg3 Attenuates TNF-α-Induced Damage in Chondrocytes through Regulating SIRT1-Mediated Anti-Apoptotic and Anti-Inflammatory Mechanisms vol.10, pp.12, 2012, https://doi.org/10.3390/antiox10121972