The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Mutagenesis of Pleurotus eryngii by Gamma Ray Irradiation

감마방사선 조사에 의한 큰느타리버섯의 돌연변이 유발

  • Kim, Jong-Kun (Graduate School of Bio. & Information Technology, Hankyong National University) ;
  • Lim, Seon-Hwa (Graduate School of Bio. & Information Technology, Hankyong National University) ;
  • Kim, Il-Joong (Graduate School of Bio. & Information Technology, Hankyong National University) ;
  • Lee, Yun-Hae (Mushroom Research Institute, GARES) ;
  • Kang, Hee-Wan (Graduate School of Bio. & Information Technology, Hankyong National University)
  • 김종군 (한경대학교 바이오.정보기술 전문대학원) ;
  • 임선화 (한경대학교 바이오.정보기술 전문대학원) ;
  • 김일중 (한경대학교 바이오.정보기술 전문대학원) ;
  • 이윤혜 (경기도농업기술원 버섯연구소) ;
  • 강희완 (한경대학교 바이오.정보기술 전문대학원)
  • Received : 2012.06.08
  • Accepted : 2012.06.25
  • Published : 2012.06.30
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Abstract

Gamma ray irradiation mutagenesis was employed to get variants of Pleurotus eryngii with functionally enhanced and improved characteristics. Protoplasts released from P. eryngii were treated with gamma ray radiation under 0.25-1.25 KGy. Protoplast sample that showed fatality rate of 80% at the 0.25 KGy was spreaded on YPMGA (yeast, peptone, malt-extract, glucose, agar) and 500 mycelial colonies were randomly selected from the medium. Of them, 100 mutant strains with mycelial morphology and growth rate that differ to control strain were observed on PDA. The cellulase and laccase activity of 67 gamma ray-irradiated P. eryngii isolates with morphological variation were investigated. Among these, 5 isolates were higher cellulase. In addition, the genetic variation of the mutant strains was analyzed by PCR fingerprinting.

본 연구는 큰느타리버섯(Pleurotus eryngii)에 감마 방사선을 조사하여 형태적, 생리적 특성이 향상된 새로운 큰느타리버섯 품종을 개발하기 위하여 수행되었다. 큰느타리버섯에서 분리한 원형질체에 0.25-1.25 KGy의 감마 방사선을 조사하였다. 원형질체는 YPMGA 배지에서 80%의 치사율을 나타냈고 이들 중 무작위로 500개의 변이체를 선발하여 PDA 배지에 배양하였다. 그 결과, 대부분의 변이체가 대조구와 비슷한 형태와 생장률을 나타냈으며, 이 중 100개의 변이체가 대조구와 약간의 차이를 보였다. 67개 변이체에 대해 cellulase 활성과 laccase 활성을 조사하였다. 그 결과, 대체적으로 대조구와 유사한 효소 활성을 나타내었고, 5개의 변이체가 대조구에 비해 더 높은 cellulase 활성을 나타내었다. 또한 감마 방사선 조사 변이체의 유전적 변이를 조사하기 위하여 UFPF-PCR을 이용하여 다형성을 조사하였다.

Keywords

References

  1. Buswell, J. A., Cai, Y. and Chang, S. 1995. Effect of nutrient nitrogen and manganese on manganese peroxidase and laccase production by Lentinula (Lentinus) edodes. FEMS Lett. 128: 81-88. https://doi.org/10.1111/j.1574-6968.1995.tb07504.x
  2. Cho, S. H., Lee, S. D., Ryu, J. S., Kim, N. G. and Lee, D. S. 2001. Changes in quality of king oyster mushroom (Pleurotus eryngii) during modified atmosphere storage. Korean J. Postharvest Sci. Technol. 8: 367-373. (in Korean).
  3. Husaini, A., Fisol, F. A., Yun, L. C., Hussain, M. H., Muid, S. and Roslan, H. A. 2011. Lignocellulolytic enzymes produced by tropical white rot fungi during biopulping of Acacia mangium wood chips. J. Biochem. Tech. 3: 245-250.
  4. Jeong, H. and Kim, K. R. 2010. Strain improvement based on ion beam-induced mutagenesis. Kor. J. Microbiol. Biotechnol. 38(3): 235-243. (in Korean).
  5. Kang, M. S., Kang, T. S., Kang, A. S., Shon, H. R. and Sung, J. M. 2000. Studies on mycelial growth and artificial cultivation of Pleurotus eryngii. Korean J. Mycol. 28: 73-80. (in Korean).
  6. Kim, J. K., Kim, J. H., Kong, W. S. and Kang, H. W. 2010. Selection of high efficient enzyme for protoplasts isolation from mushrooms. Kor. J. Mycol. 38(1): 21-24. (in Korean). https://doi.org/10.4489/KJM.2010.38.1.021
  7. Kim, J. K., Moon, D. H., Seo, G. S. and Kang, H. W. 2011. Mutagenesis of Hypsizygus marmoreus by gamma ray irradiation. Kor. J. Mycol. 39(3): 231-234. (in Korean). https://doi.org/10.4489/KJM.2010.39.3.231
  8. Lee, Y.-K., Chang, H.-H., Kim, J.-S., Jang, Y. S. and Lee, H. Y. 2002. Gamma radiation induced mutational spectrum of laccase gene in Pleurotus ostreatus. Korean J. Environ. Biol. 20(4): 316-324.
  9. Lee, Y.-K., Chang, H.-H., Kim, J.-S., Kim, J. K. and Lee, K.-S. 2000. Lignocellulolytic mutants of Pleurotus ostreatus induced by gamma-ray radiation and their genetic similarities. Radiation Physics and Chemistry 57(2): 145-150. https://doi.org/10.1016/S0969-806X(99)00310-2
  10. Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428. https://doi.org/10.1021/ac60147a030
  11. Ministry for food, agriculture, forestry and fisheries republic of Korea. 2011. The actual output of special crop. pp. 10.
  12. Revankar, M. and Lele, S. S. 2006. Enhanced production of laccase using a new isolate of white rot fungus WR-1. Process Biochemistry. 41: 581-588. https://doi.org/10.1016/j.procbio.2005.07.019
  13. Rural Resources Development Institute. 2002. Food composition table. pp. 154. (in Korean).
  14. Stamets, P. 1993. Growing gourmet and medicinal mushrooms. Ten Speed Press. Hong Kong. pp.304-308.

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