Toxicological Research

  • 제28권2호
  • /
  • Pages.117-122
  • /
  • 2012
  • /
  • 1976-8257(pISSN)
  • /
  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
권호 표지
DOI QR코드

DOI QR Code

Increased Flavonoid Compounds from Fermented Houttuynia cordata using Isolated Six of Bacillus from Traditionally Fermented Houttuynia cordata

  • Kwon, Ryun-Hee (Department of Pharmaceutical Engineering and College of Medical Life Science, Silla University) ;
  • Ha, Bae-Jin (Department of Pharmaceutical Engineering and College of Medical Life Science, Silla University)
  • 투고 : 2012.06.01
  • 심사 : 2012.06.27
  • 발행 : 2012.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Flavonoids, which form a major component in Houttuynia cordata Thunb., display a wide range of pharmacological activities. The expression of plant flavonoids is partly regulated by fermentation. Therefore, we studied the effects of fermentation on H. cordata in order to identify the strains present during the fermentation process, and to determine whether fermented H. cordata could be used as a probiotic. Our results showed that all 6 of the bacterial strains isolated from fermented H. cordata (FHC) belonged to the genus Bacillus. As expected, fermenting H cordata also increased the flavonoid content as increases were observed in the levels of rutin, quercitrin, and quercetin. To test the effects of fermentation, we treated LPS-stimulated RAW264.7 cells with non-fermented H. cordata extracts (HCE) or FHC extracts (FHCE). Compared to the HCE-treated cells, the FHCE-treated cells showed increased viability. No cytotoxic effects were detected in the FHCE-treated groups in the 2 cell lines used in the study, namely, RAW264.7 and RBL-2H3. FHCE-treated HepG2 cells showed decreased growth, compared to HCE-treated HepG2 cells. These results indicate that the fermented H. cordata predominantly contained Bacillus strains. Furthermore, FHCE are able to prevent LPS-induced inflammatory effects and inhibit the growth of HepG2 cells.

키워드

참고문헌

  1. Beker, B.Y., Bakir, T., Sonmezoglu, I., Imer, F. and Apak, R. (2011). Antioxidant protective effect of flavonoids on linoleic acid peroxidation induced by copper(II)/ascorbic acid system. Chem. Phys. Lipids., 164, 732-739. https://doi.org/10.1016/j.chemphyslip.2011.09.001
  2. Chen, X., Wang, Z., Yang, Z., Wang, J., Xu, Y., Tan, R.X. and Li, E. (2011). Houttuynia cordata blocks HSV infection through inhibition of NF-${\kappa}$B activation. Antiviral Res., 92, 341-345. https://doi.org/10.1016/j.antiviral.2011.09.005
  3. Fardet, A., Rock, E. and Remesy, C. (2008). Is the in vitro antioxidant potential of whole-grain cereals and cereal products well reflected in vivo?. J. Cereal Sci., 48, 258-276. https://doi.org/10.1016/j.jcs.2008.01.002
  4. Gaggìa, F., Mattarelli, P. and Biavati, B. (2010). Probiotics and prebiotics in animal feeding for safe food production. Int. J. Food Microbiol., 141, S15-S28. https://doi.org/10.1016/j.ijfoodmicro.2010.02.031
  5. Hall, T.A. (1999). Bio-Edit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp., 41, 95-98.
  6. He, N., Yang, X., Jiao, Y., Tian, L. and Zhao, Y. (2012). Characterisation of antioxidant and antiproliferative acidic polysaccharides from Chinese wolfberry fruits. Food Chem., 133, 978-989. https://doi.org/10.1016/j.foodchem.2012.02.018
  7. Higgins, S.E., Erf, G.F., Higgins, J.P., Henderson, S.N., Wolfenden, A.D., Gaona-Ramirez, G. and Hargis, B.M. (2007). Effect of probiotic treatment in broiler chicks on intestinal macrophage numbers and phagocytosis of Salmonella enteritidis by abdominal exudate cells. Poult. Sci., 86, 2315-2321. https://doi.org/10.3382/ps.2007-00123
  8. Lau, K.M., Lee, K.M., Koon, C.M., Cheung, C.S., Lau, C.P., Ho, H.M., Lee, M.Y., Au, S.W., Cheng, C.H., Lau, C.B., Tsui, S.K., Wan, D.C., Waye, M.M., Wong, K.B., Wong, C.K., Lam, C.W., Leung, P.C. and Fung, K.P. (2008). Immunomodulatory and anti-SARS activities of Houttuynia cordata. J. Ethnopharmacol., 118, 79-85. https://doi.org/10.1016/j.jep.2008.03.018
  9. Leonard, E., Yan, Y. and Koffas, M.A. (2006). Functional expression of a P450 flavonoid hydroxylase for the biosynthesis of plant-specific hydroxylated flavonols in Escherichia coli. Metab. Eng., 8, 172-181. https://doi.org/10.1016/j.ymben.2005.11.001
  10. Meng, J., Leung, K.S., Dong, X.P., Zhou, Y.S., Jiang, Z.H. and Zhao, Z.Z. (2009). Simultaneous quantification of eight bioactive components of Houttuynia cordata and related Saururaceae medicinal plants by on-line high performance liquid chromatography- diode array detector-electrospray mass spectrometry. Fitoterapia, 80, 468-474. https://doi.org/10.1016/j.fitote.2009.06.013
  11. Niki, E. (2010). Assessment of Antioxidant Capacity in vitro and in vivo. Free Radic. Biol. Med., 49, 503-515. https://doi.org/10.1016/j.freeradbiomed.2010.04.016
  12. Nuengchamnong, N., Krittasilp, K. and Ingkaninan, K. (2009). Rapid screening and identification of antioxidants in aqueous extracts of Houttuynia cordata using LC-ESI-MS coupled with DPPH assay. Food Chem., 117, 750-756. https://doi.org/10.1016/j.foodchem.2009.04.071
  13. Oboh, G., Alabi, K.B. and Akindahunsi, A.A. (2008). Fermentation changes the nutritive values, polyphenol distribution, and antioxidant properties of Parkia giglobosa seeds (African locust beans). Food Biotechnol., 22, 363-376. https://doi.org/10.1080/08905430802463404
  14. Patel, A.K., Ahire, J.J., Pawar, S.P., Chaudhari, B.L., Shouche, Y.S. and Chincholkar, S.B. (2010). Evaluation of Probiotic Characteristics of Siderophoregenic Bacillus spp. Isolated from Dairy Waste. Appl. Biochem. Biotechnol., 160, 140-155. https://doi.org/10.1007/s12010-009-8583-2
  15. Philip, K., Sinniah, S.K. and Muniandy, S. (2009). Antimicrobial peptides in aqueous and ethanolic extracts from microbial, plant and fermented sources. Biotechnology, 8, 248-253. https://doi.org/10.3923/biotech.2009.248.253
  16. Taira, J., Nanbu, H. and Ueda, K. (2009). Nitric oxide-scavenging compounds in Agrimonia pilosa Ledeb on LPS-induced RAW264.7 macrophages. Food Chem., 115, 1221-1227. https://doi.org/10.1016/j.foodchem.2009.01.030
  17. Tian, L., Zhao, Y., Guo, C. and Yang. X. (2011). A comparative study on the antioxidant activities of an acidic polysaccharide and various solvent extracts derived from herbal Houttuynia cordata. Carbohydr. Polymer., 83, 537-544. https://doi.org/10.1016/j.carbpol.2010.08.023
  18. Wu, L.S., Si, J.P., Yuan, X.Q. and Shi X.R. (2009). Quantitive Variation of Flavonoids in Houttuynia cordata from Different Geographic Origins in China. Chinese Journal of Natural Medicines, 7, 40-46. https://doi.org/10.3724/SP.J.1009.2009.00040

피인용 문헌

  1. Antimicrobial Dyeing of Cotton and Silk Fabrics Using Houttuynia cordata Extract vol.27, pp.3, 2015, https://doi.org/10.5764/TCF.2015.27.3.194
  2. Antiproliferative activities and phenolic acid content of water and ethanolic extracts of the powdered formula of Houttuynia cordata Thunb. fermented broth and Phyllanthus emblica Linn. fruit vol.18, pp.1, 2018, https://doi.org/10.1186/s12906-018-2185-x
  3. Herbal Approach for Management of Atherosclerosis: a Review vol.21, pp.4, 2019, https://doi.org/10.1007/s11883-019-0774-x