한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제41권6호
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  • Pages.782-789
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  • 2012
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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칡(Pueraria thunbergiana)추출물 청국장 캡슐의 ob/ob Mice에 대한 항비만 효과

Anti-obesity Effects of Capsule-filled Cheonggukjang Added with Arrowroot (Pueraria thunbergiana) Extracts in ob/ob Mice

  • 김길수 (경북대학교 수의과대학) ;
  • 홍주헌 (대구가톨릭대학교) ;
  • 김대익 ((재)대구테크노파크 바이오헬스융합센터)
  • Kim, Kil-Soo (College of Veterinary Medicine, Kyungpook National University) ;
  • Hong, Joo-Heon (Dept. of Food Science and Technology, Catholic University) ;
  • Kim, Dae-Ik (Daegu Technopark Bio Health Convergence Center)
  • 투고 : 2012.02.23
  • 심사 : 2012.05.07
  • 발행 : 2012.06.30
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초록

본 연구는 칡청국장 및 칡청국장 캡슐함유 식이가 체중, 지방축적, 지질대사 등에 미치는 효능을 알아보기 위하여 선천성 비만 및 인슐린 비의존성 당뇨병의 모델동물인 ob/ob 마우스를 대상으로 정상대조군(NC: 일반식이군), 양성대조군(PC: 2% 청국장함유군), 칡청국장 2% 함유군(AR: 2% 칡청국장), 칡청국장 캡슐군(ARC: 2% 칡청국장 캡슐함유)으로 나누어 10주간 실험하였다. 체중 및 식이효율은 칡청국장 캡슐군에서 정상대조군에 비하여 유의하게 감소하였다. 혈청 콜레스테롤, LDL-콜레스테롤, 중성지방, 혈당 및 동맥경화지수는 칡청국장 캡슐군에서 정상대조군에 비하여 유의하게 감소하였다. 칡청국장 캡슐군에서 지방간의 전형적인 지방축적의 억제효과를 보였다. 간조직의 SOD, CAT, GST 활성은 칡청국장 캡슐군에서 정상대조군에 비하여 유의하게 증가하였다. 이에 칡 추출물 청국장 및 캡슐형태의 투여는 지방함량의 저하효과, 혈당강하효과, 항산화계의 활성도 증가 등의 효과를 통하여 항비만기능이 있으며, 칡청국장 캡슐형의 기능성이 높다는 사실을 확인할 수 있었다.

This study was designed to evaluate the anti-metabolic syndrome effects of capsule-filled cheonggukjang (CGJ) added with arrowroot (Pueraria thunbergiana) extracts on body weight, adiposity and lipid metabolism in ob/ob mice. Experimental groups were normal control group (NC: basal diet), positive control group (PC: 2% CGJ), CGJ added with arrowroot extracts group (AR: 2% arrowroot in CGJ), and capsule-filled CGJ added with arrowroot extracts group (ARC: 2% arrowroot CGJ capsule). Each group was fed experimental diet for 10 weeks. Final body weight gain and atherogenic index were significantly lower in the ARC than NC group. Serum levels of total cholesterol, LDL-cholesterol, and triglycerides, blood glucose and atherogenic index were significantly lower in the ARC than NC group. Furthermore, fatty liver and regional lipid accumultion in ob/ob mice were inhibited in the ARC group. The hepatic activities of superoxide dismutase, catalase and glutathione S-transferase were significantly higher in the ARC than NC group. Therefore, the anti-matabolic syndrome effects of the ARC group were higher than the AR group. In conclusion, these results indicated that CGJ added with arrowroot mediates its anti-obesity effects in ob/ob mice by improving lipid metabolism and antioxidant enzyme.

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참고문헌

  1. Yeo JH, Chon JW, Lee KG, Woo SO, Nam SH, Kweon HY, Han SM, Jo YY, Lee HS, Lee MR, Choi YS, Park YK. 2009. Effect of weight loss and lipid metabolism in diet-induced obesity mice by Korean mixed pollen. Korean J Apiculture 24: 191-199.
  2. Kim CS. 2000. Amylin analogue in the treatment of obesity. The Korean J Obesity 19: 39-48.
  3. World Health Organization. 2000. Obesity: preventing an managing the global epidemic. Report of a WHO Consultation. World Health Organization (WHO technical report series 894), Geneva, Switzerland.
  4. Lee SK, So SH, Hwang EI, Koo BS, Han GH, Ko SB, Kim NM. 2008. Effect of ginseng and herbal plant mixtures on anti-obesity in obese SD rat induced by high fat diet. J Korean Soc Food Sci Nutr 37: 437-444. https://doi.org/10.3746/jkfn.2008.37.4.437
  5. Yang CY, Cho MJ, Lee CH. 2011. Effect of fermented turmeric extracts on the obesity in rats fed a high-fat diet. J Anim Sci Tech 53: 75-81. https://doi.org/10.5187/JAST.2011.53.1.75
  6. Lee JJ, Kim AR, Lee H, KIM CH, Chang HC, Lee MY. 2011. Effect of soybean, cheonggukjang and doenjang on serum cholesterol level and weight reduction in rats fed a highfat/high-cholesterol diet. Korean J Food Preserv 18: 226-235. https://doi.org/10.11002/kjfp.2011.18.2.226
  7. Tanaka K, Aso B, Sugano M. 1984. Biliary steroid excretion in rats fed soybean protein and casein or their amino acid mixtures. J Nutr 114: 26-32.
  8. Phinney SD, Odin RS, Johnson SB, Holman RT. 1990. Reduced arachidonate in serum phospholipids and cholesteryl esters associated with vegetarian diets in human. Am J Clin Nutr 51: 385-392.
  9. Oh MJ, Son HY, Kang JC, Lee GS. 1990. Antioxidative effect of pueraria root extract on edible oils and fats. J Korean Soc Food Nutr 19: 448-456.
  10. Tesung KY, Chou YP, Chang LY, Fan LL. 1975. Pharmacological studies on Radix Puerariae I. Chin Med J Engl 1: 335-342.
  11. Fan LL, Dennis DO, Wand WJP. 1985. Pharmacologic studies on Radix puerariae. Effect of puerariae on reginal myocardial blood-flow and cardic hemodynamics in dogs with acute myocardial ischemia. Chin Med J 98: 821-832.
  12. Haglund O, Loustarinen R, Wallin R, Wibell I, Saldeen T. 1991. The effect of fish oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in mans supplemented with vitamin. Eur J Nutr 121: 165-172.
  13. Ellman GL. 1959. Tissue sulfhydryl group. Arch Biochem Biophys 82: 70-77. https://doi.org/10.1016/0003-9861(59)90090-6
  14. Ohkawa H, Ohishi N, Yake K. 1979. Assay fro lipid peroxides in animal tissues by thiobarbituric acid reaction. Annal Biochem 95: 351-358. https://doi.org/10.1016/0003-2697(79)90738-3
  15. Stirpe F, Della CE. 1969. The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) oxidase (type O). J Biol Chem 244: 3855-3863.
  16. Martin JP, Dailey M, Sugarman E. 1987. Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Arch Biochem Biophys 255: 329-336. https://doi.org/10.1016/0003-9861(87)90400-0
  17. Aebi H. 1974. Catalase. In Methods of Enzymatic Analysis. Bergmeyer HU, ed. Academic Press, New York, NY, USA. Vol 1, p 674.
  18. Habig WH, Pabst MJ, Jakoby WB. 1974. Glutathione Stransferase. The first enzymatic step in mercapturic acid and formation. J Biol Chem 249: 7130-7139.
  19. Ingalls AM, Dickie MM, Snell GD. 1950. Obese a new mutation in the house mouse. J Hered 41: 317-318. https://doi.org/10.1093/oxfordjournals.jhered.a106073
  20. Park ST, Jeong JC. 2008. Effects of Cheonghyul-san on blood glucose, hyperlipidemia, polyol pathway and reactive oxygen species in ob/ob mice. Korean J Oriental Physiology and Pathology 22: 350-356.
  21. Kleinman JC, Donahue RP, Harris MI, Finrcane FF, Madaus JH, Brock DB. 1988. Mortality among diabetes in a national sample. Am J Epidemiol 128: 389-401. https://doi.org/10.1093/oxfordjournals.aje.a114979
  22. Pesce AJ, Kaplan LA. 1987. Methods in Clinical Chemistry. CV Mosby Co, St. Louis, MO, USA. p 1074-1079.
  23. Aloia JF, Cohr SH, Vaswani A, Yeh JK, Yuen K, Ellis K. 1985. Risk factors for postmenopausal osteoporosis. Am J Med 78: 95-100.
  24. Moon SJ, Kim JH, Lim SK. 1996. Investigation of risk of low serum 25-hydroxyvitamin D levels in Korean menopausal women. Korean J Nutr 29: 981-990.
  25. Kim HJ, Kim YC. 2006. Antidiabetic and antioxidant effects of chunggugjang powder in streptozotocin-induced diabetic rats. J Environ Toxicol 21: 139-246.
  26. Saito M. 1988. Interaction between lipid peroxide formation and nutritional status. J Jpn Soc Nutr Food Sci 41: 343-349. https://doi.org/10.4327/jsnfs.41.343
  27. Im MJ, Manson PN, Bulkley GB, Hoopes JE. 1985. Effects of superoxide dismutase and allopurinol on the survival of acute island skin flaps. Ann Surg 201: 357-359. https://doi.org/10.1097/00000658-198503000-00018
  28. Manson PN, Anthenelli RM, Im MJ, Bulkley GB, Hoopes JE. 1983. The role of oxygen free radicals in ischemic tissue injury in island skin flaps. Ann Surg 198: 87-90. https://doi.org/10.1097/00000658-198307000-00017
  29. Morel DW, Chisolm GM. 1989. Antioxidative treatment of diabetic rats inhibits lipoprotein oxidation and cytotoxicity. J Lipid Res 30: 1827-1834.
  30. Deisseroth A, Dounce AL. 1970. Catalase physical and chemical properties, mechanism of catalysis and physiological role. Physiol Rev 50: 3-24.

피인용 문헌

  1. Anti-Obesity Effect of By-Product from Soybean on Mouse Fed a High Fat Diet vol.28, pp.2, 2015, https://doi.org/10.7732/kjpr.2015.28.2.168