한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제41권6호
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  • Pages.739-744
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  • 2012
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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국내 자생 엉겅퀴추출물의 항산화 성분 및 활성

Antioxidative Components and Activity of Domestic Cirsium japonicum Extract

  • 장미란 (덕성여자대학교 식물자원연구소) ;
  • 홍은영 (CJ제일제당(주) 식품연구소) ;
  • 정재훈 (삼육대학교 의명신경과학연구소) ;
  • 김건희 (덕성여자대학교 식물자원연구소)
  • Jang, Mi-Ran (Plant Resources Research Institute, Duksung Women's University) ;
  • Hong, Eun-Young (Foods R&D, CJ CheilJ edang Corporation) ;
  • Cheong, Jae-Hoon (Uimyung Research Institute for Neuroscience, Sahmyook University) ;
  • Kim, Gun-Hee (Plant Resources Research Institute, Duksung Women's University)
  • 투고 : 2012.02.24
  • 심사 : 2012.06.06
  • 발행 : 2012.06.30
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초록

본 연구는 자생분포가 높은 4가지 지역(경기, 강원, 전남 및 제주)에서 채집한 엉겅퀴를 에탄올 추출하여 총 폴리페놀과 총 플라보노이드 함량, DPPH 및 ABTS radical 소거능, 2가지 방법에 의한 환원력을 분석하여 새로운 자생식물 유래의 항산화제 소재개발에 있어 원물 최적화를 위하여 시행하였다. 총 폴리페놀 및 플라보노이드를 많이 함유하고 있을수록 4가지 항산화능의 평가 역시 효과적인 것으로 나타났다. 이는 총 폴리페놀 및 플라보노이드 함량과 항산화 활성의 유의적인 상관성이 있음을 확인할 수 있는 결과라 사료된 다. 엉겅퀴는 현재 잘 소비되는 식물자원은 아니지만 다양한 효능이 확인되고 있는 엉겅퀴를 이용한 소재 및 제품 개발을 위해서는 본 연구결과를 활용한 최적의 재배지역을 선정하고, 향후 재배기술 및 원물의 규격화를 위한 연구는 반드시 수행되어야 할 것으로 판단된다.

Cirsium japonicum (Gyonggido (GG), Gangwondo (GW), Jeollanamdo (JN) and Jejudo (JJ)) were investigated for their antioxidative components (total polyphenol and flavonoid contents) and their antioxidant activities (2,2-diphenyl-1-picrylhydrazyl) (DPPH), 2,2'-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP) and reducing power). Analysis of antioxidative components and activities showed significant (p<0.05) differences across samples from various regions (GG, GW, JN, JJ). Amounts of total polyphenol and flavonoid contents were in the order of GG${\geq}$GW>JJ>JN (p<0.05). The results of the antioxidative activity test of Cirsium japonicum showed that GG and GW had higher activities compared to others (p<0.05). Therefore, the results of the antioxidative activity test (DPPH, ABTS, FRAP and reducing power) were correlated with total polyphenol and flavonoid contents values.

키워드

참고문헌

  1. Choi YJ. 1987. Cultivation and Utilization of Wild Edible . Osung Press, Seoul, Korea. p 337.
  2. Lee SJ. 1966. Korean Folk Medicine . Seoul National University Press, Seoul, Korea. p 145-146.
  3. Ishida H, Umino T, Tsuji K, Kosuge T. 1987. Studies on antihemorrhagic substance in herbs classified as hemostatics in Chinese medicine VII. On the antihemorrahgic principle in Cirsium japonicum DC. Chem Pharm Bull 35: 861-864. https://doi.org/10.1248/cpb.35.861
  4. Kim SJ, Kim GH. 2003. Identification for flavones in different parts of Cirsium japonicum. J Food Sci Nutr 8: 330-335. https://doi.org/10.3746/jfn.2003.8.4.330
  5. Fang J, Xia C, Cao Z, Zheng JZ, Reed E, Jiang BH. 2005. Apigenin inhibits VEGF and HIF-1 expression via PI3K/ AKT/p70S6K1 and HDM2/p53 pathways. FASEB J 19:3423.
  6. Lee HK, Kim JS, Kim NY, Kim MJ, Park SU, Yu CY. 2003. Antioxidant, antimutagenicity and anticancer activities of extracts from Cirsium japonicum var. ussuriense KITAMURA. Kor J Medicinal Crop Sci 11: 53-61.
  7. Lee MK, Moon HC, Lee JH, Kim JD, Yu CY, Lee HY. 2002. Screening of immune enhancing activities in medicinal herbs, Compositae . Kor J Medicinal Crop Sci 10: 51-57.
  8. Liu S, Luo X, Li D, Zhang J, Qui D, Liu W, She L, Yang Z. 2006. Tumor inhibition and improved immunity in mice treated with flavone from Cirsium japonicum DC. Int Immunopharmacol 6: 1387-1393. https://doi.org/10.1016/j.intimp.2006.02.002
  9. Park HK, Yoon SY, Choi JH, Ko HS, Suh YW, Lee YS, Kim GH, Chung MS, Cheong JH. 2006. The antidepressant effects of Cirsium japonicum in ICR mice. J Pharm Soc Korea 50: 429-435.
  10. Lim DK, Choi U, Shin DH. 1996. Antioxidative activity of ethanol extract from Korean medicinal plants. Korean J Food Sci Technol 28: 83-89.
  11. Folin O, Denis W. 1912. On phosphotungastic-phosphomolybdic compounds as color reagents. J Biol Chem 12: 239-249.
  12. Ko SH, Kim SI, Han YS. 2008. The quality characteristics of yogurt add supplemented with low grade dried-persimmon extracts. Kor J Food Cookery Sci 24: 735-741.
  13. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  14. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  15. Oyaizu M. 1986. Studies on products of browning reactions; antioxidant activities of products of browning reaction prepared from glucose amine. Jpn J Nutr 44: 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  16. Benzie IFF, Strain JJ. 1996. The Ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal Biochem 239: 70-76. https://doi.org/10.1006/abio.1996.0292
  17. Beecher GR. 2003. Overview of dietary flavonoids: nomenclature, occurrence and intake. J Nutr 133: 3248S-3254S.
  18. Jeong JA, Kwon SH, Lee CH. 2007. Screening for antioxidative activities of extracts from aerial and underground parts of some edible and medicinal ferns. Kor J Plant Res 20: 185-192.
  19. Villano D, Fernandez-Pachon MS, Moya ML, Troncoso AM, Garcia-Parrilla MC. 2007. Radical scavenging ability of polyphenolic compounds towards DPPH free radical. Talanta 71: 230-235. https://doi.org/10.1016/j.talanta.2006.03.050
  20. Choi Y, Jeong HS, Lee J. 2007. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem 103: 130-138. https://doi.org/10.1016/j.foodchem.2006.08.004
  21. Park SII, Kim KS. 2004. Isolation and identification of antioxidant flavonoids from Salicornia herbacea L. J Kor Soc Appl Biol Chem 47: 120-123.
  22. Wang MF, Shao Y, Li JG, Zhu NQ, Rangarajan M, Lavoic EJ, Ho CT. 1998. Antioxidative phenolic compounds from sage (Salvia officinalis). J Agric Food Chem 46: 4869-4873. https://doi.org/10.1021/jf980614b
  23. Kim I, Jeong C, Park S, Shim K. 2011. Nutritional components and antioxidative activities of jujube (Zizyphus jujuba) fruit and leaf. Korean J Food Preserv 18: 341-348 https://doi.org/10.11002/kjfp.2011.18.3.341

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  9. Analysis of Pectolinarin Content and Antioxidant activity in Cirsium setidens Nakai by Cultivars vol.31, pp.3, 2016, https://doi.org/10.13103/JFHS.2016.31.3.210
  10. Physicochemical Characteristics and Antioxidant Activities of Organic Strawberries vol.30, pp.6, 2015, https://doi.org/10.7318/KJFC/2015.30.6.773
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  13. Development of fermented beverage using corn silk and mulberry leaf extracts vol.28, pp.2, 2012, https://doi.org/10.11002/kjfp.2021.28.2.199