Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Behavioral and Physiological Responses of Juvenile Red Seabream Pagrus major exposed to Ethanol Seawater

에탄올에 대한 참돔 Pagrus major의 행동 및 생리학적 반응

  • Park, Jin-Woo (Department of Aquatic Life Medical Science, Sunmoon University) ;
  • Chang, Young-Jin (Department of Marine Bio-materials and Aquaculture, Pukyong National University) ;
  • Kim, Ki-Tae (Department of Marine Bio-materials and Aquaculture, Pukyong National University) ;
  • Kwon, Joon-Yeong (Department of Aquatic Life Medical Science, Sunmoon University)
  • 박진우 (선문대학교 수산생명의학과) ;
  • 장영진 (부경대학교 해양바이오신소재학과) ;
  • 김기태 (부경대학교 해양바이오신소재학과) ;
  • 권준영 (선문대학교 수산생명의학과)
  • Received : 2011.10.26
  • Accepted : 2012.02.27
  • Published : 2012.04.30
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Abstract

Behavioral and physiological responses of juvenile red seabream (Pagrus major) to different concentrations of ethanol were investigated. No swimming and no reaction to touching by a wooden stick was observed at 0.6% ethanol group in behavioral response, and survival rate was 100% after 5 hours of treatment. Red blood cell count, hematocrit and hemoglobin levels in plasma were not significantly different among all groups. AST activities in plasma significantly decreased as ethanol concentration increased. On the contrary, ALT activities in plasma significantly increased as ethanol concentration increased. Cortisol level in plasma was the lowest in 0.6% ethanol group. Glucose levels in plasma increased significantly when ethanol concentration increased more than 0.4%. Oxygen consumption of fish in 0.6% ethanol seawater was constantly lower than that of fish in control seawater from 2 hours after the exposure to ethanol seawater until the end of experiment.

에탄올 0.6% 해수에 5시간 동안 침지한 참돔 치어는 유영행동을 멈추었으며 외부의 물리적 자극에도 반응하지 않는 상태에 도달하였다. 이 농도구에서 생존율은 100%였으며 RBC, hematocrit, Hb 수치는 다른 그룹과 유의한 차이를 보이지 않았다. 혈장 코티졸 농도 또한 0.6% 에탄올 농도에서 가장 낮은 값을 나타냈다. 그러나 0.8% 이상의 에탄올 해수에 침지한 실험어는 심한 스트레스 반응과 높은 폐사율을 보였다. 참돔 치어를 0.6%의 에탄올 해수에 침지시키고 매시간 산소소비량을 측정한 결과, 에탄올해수에 침지된 실험어는 실험개시 2시간 후부터 종료 시까지 지속적으로 일반해수에 침지된 실험어보다 적은 산소를 소비하였다. 이상의 결과들은 에탄올에 의해 유도된 취기효과가 마취제의 마취효과와 비슷하였으며, 5시간 침지 시 혈액성상, 행동반응, 생존율, 산소소비율 등을 종합하여 볼 때, 0.6% 이하의 에탄올 농도가 참돔치어에게 안전한 농도이며, 0.8% 이상에서는 독성으로 작용할 수 있음을 보여준다.

Keywords

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