The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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The Protective Effects of Chrysanthemum cornarium L. var. spatiosum Extract on HIT-T15 Pancreatic β-Cells against Alloxan-induced Oxidative Stress

Alloxan에 의한 HIT-T15 세포 손상에 대한 쑥갓주정추출물의 세포보호효과

  • Kim, In-Hye (Functional Food & Nutrition Division, Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Cho, Kang-Jin (Functional Food & Nutrition Division, Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ko, Jeong-Sook (Functional Food & Nutrition Division, Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Jae-Hyun (Functional Food & Nutrition Division, Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Om, Ae-Son (Dept. of Food and Nutrition, College of Human Ecology, Hanyang University)
  • 김인혜 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 조강진 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 고정숙 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 김재현 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 엄애선 (한양대학교 생활과학대학 식품영양학과)
  • Received : 2012.01.25
  • Accepted : 2012.03.20
  • Published : 2012.03.31
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Abstract

The objective of the present study was to evaluate the potential antidiabetic and antioxidant effect of the ethanol extract from Chrysanthemum cornarium L. var. spatiosum(CSE) against alloxan-induced oxidative stress in pancreatic ${\beta}$-cells, HIT-T15. In this study, the antidiabetic effect of CSE was examined using the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliu bromide(MTT) cell proliferation assay, lactate dehydrogenase(LDH) release assay, $NAD^+$/NADH ratio and insulin secretion. To further investigate whether CSE is involved in the antioxidant activity of alloxan-damaged HIT-T15 cells, its antioxidant effect against alloxan-induced oxidative stress was measured in HIT-T15 cells by determining the levels of antioxidant enzymes including superoxide dismutase(SOD), glutathione S-transferase(GST), glutathione reductase(GR) and glutathione peroxidase(GPx). The results of this analysis showed that alloxan significantly decreased cell viability, increased LDH leakage, and lowered $NAD^+$/NADH ratio and insulin secretion in HIT-T15 cells. However, CSE significantly increased the viability of alloxan-treated cells and lowered LDH leakage. The intracellular NAD+/NADH ratio and insulin secretion were also significantly increased by 1.7-fold and 1.3-fold, respectively, after treatment with 100 ${\mu}g/m{\ell}$ CSE. The HIT-T15 cells treated with alloxan showed significant decreases in the activities of antioxidant enzymes, while CSE significantly elevated the levels of antioxidant enzymes. These findings suggest that CSE could have a protective effect against cytotoxicity and dysfunction of pancreatic cells in the presence of alloxan-induced oxidative stress.

Keywords

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