DOI QR코드

DOI QR Code

Enhancement of Low Molecular Ginsenoside Contents in Low Quality Fresh Ginseng by Fermentation Process

등외품 인삼(파삼)의 유산균 발효에 의한 저분자 진세노사이드 함량 증진

  • Received : 2011.12.12
  • Accepted : 2012.04.05
  • Published : 2012.04.30

Abstract

This study compared the contents of low molecular ginsenoside according to fermentation process in low grade fresh ginseng. Low grade fresh ginseng was directly inoculated with a 24 h seed culture of $Bifidobacterium$ Longum B6., $Lactobacillus$ $casei$., and incubated at $36^{\circ}C$ for 72 h. $Bifidobacterium$ Longum B6 was specifically was found to show the best growth on $3,255{\times}10^6\;CFU/m{\ell}$ after 48 h of fermentation. The content of ginsenoside Rb1, Re and Rd were decreased with the fermentation but ginsenoside Rh2 and Rg2 increased after fermentation process. In the case of low molecular ginsenoside conversion yields were 56.07% of Rh2, 12.03% of Rg3 and 77.11% of Rg2, respectively. In addition, compound-K was irregular conversion yield as long as 72 h of fermentation. This results indicate that fermentation process could increase the low molecular ginsenoside in low grade fresh ginseng.

Keywords

References

  1. Bao HY, Zhang J, Yeo SJ, Myung CS, Kim HM, Kim JM, Park JH, Cho JS and Kang JS. (2005). Memory enhancing and neuroprotective effects of selected ginsenosides. Archives of Pharmacal Research. 28:335-342. https://doi.org/10.1007/BF02977802
  2. Benishin CG. (1992). Actions of ginsenoside Rb1 on choline uptake in central cholinergic nerve endings. Neurochemistry International. 21:1-5. https://doi.org/10.1016/0197-0186(92)90061-U
  3. Doh ES, Chang JP, Lee KH and Seong NS. (2007). Ginsenoside change and antioxidation activity of fermented ginseng. Korean Journal of Medicinal Crop Science. 18:255-265.
  4. Hasegawa H, Sung JH and Yoshimi B. (1997). Role of human intestinal Prevotella oris in hydrolyzing ginseng saponins. Planta Medica. 63:436-440. https://doi.org/10.1055/s-2006-957729
  5. Hasegawa H. (2004). Proof of the mysterious efficacy of ginseng: Basic and clinical trials: Metabolic activation of ginsenoside: deglycosylation by intestinal bacteria and esterification with fatty acid. Journal of Pharmacological Sciences. 95:153-157. https://doi.org/10.1254/jphs.FMJ04001X4
  6. Hong HD, Kim YC, Rho JH, Kim KT and Lee YC. (2007). Changes on physicochemical properties of Panax ginseng C. A. Meyer during repeated steaming process. Journal of Ginseng Research. 31:175-242. https://doi.org/10.5142/JGR.2007.31.4.175
  7. Jun HK and Kim SH. (1982). Studies of the physiological activity of korean ginseng (Part2): The effects of ginseng saponin on the antimicrobial activity of antibiotics. Korean Journal of Applied Microbiology and Bioengineering. 10:163-169.
  8. Keum YS, Park KK, Lee JM, Chun KS, Park JH, Lee SK, Kwon H and Surh YJ. (2000). Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng. Cancer Letters. 150:41-48. https://doi.org/10.1016/S0304-3835(99)00369-9
  9. Kim SE, Lee YH, Park JH and Lee SK. (1999). Ginsenoside-Rs3, a new diol-type ginseng saponin, selectively elevates protein levels of p53 and p21WAF1 leading to induction of apoptosis in SK-HEP-1 cells. Anticancer Research. 19:487-491.
  10. Kim WY, Kim JM, Han SB, Lee SK, Kim ND, Park MK, Kim CK and Park JH. (2000). Steaming of ginseng at high temperature enhances biological activity. Journal of Natural Products. 63:1702-1704. https://doi.org/10.1021/np990152b
  11. Ko JH, Kim YS, Kim HY, Ra KJ, Do JH, Park JD, Park JG, Park HJ, Beak NI, Lee JH, Lee HO and Jung GT. (1994). Korean ginseng. In Park et al., (ed.) Korea and Ginseng & Tobacco Research Institute. p. 63-84.
  12. Lee NR, Han JS, Kim JS and Choi JE. (2011). Effect of extraction temperature and time on ginsenoside content and quality in ginseng (Panax ginseng) flower water extract. Korean Journal of Medicinal Crop Science. 19:271-275. https://doi.org/10.7783/KJMCS.2011.19.4.271
  13. Li XG. (1992). Studies on the transforming mechanism of amino acid components in ginseng in the course of ginseng process. Journal of Ginseng Research. 16:64-67.
  14. Noh KH, Son JW, Kim HJ and Oh DK. (2009). Ginsenoside compound K production from ginseng root extract by a thermostable ${\beta}$-glucosidase from Sulfolobus solfataricus. Bioscience Biotechnology and Biochemistry. 73:316-321. https://doi.org/10.1271/bbb.80525
  15. Park SJ, Kim DH, Paek NS and Kim SS. (2007). Preparation and quality characteristics of the fermentation product of ginseng by lactic acid bacteria (FGL). Journal of Ginseng Research. 30:57-99.
  16. Singh VK, Agarwal SS and Gupta BM. (1984). Immunomodulatory activity of Panax ginseng extract. Planta Medica. 50:462-465. https://doi.org/10.1055/s-2007-969773
  17. Yang SJ, Woo KS, Yoo JS, Kang TS, Noh YH, Lee JS and Jeong HS. (2006). Change of korean ginseng components with high temperature and pressure treatment. Korean Society of Food Science and Technology. 38:521-523.

Cited by

  1. Ginsenoside Composition and Quality Characteristics of Red Ginseng Extracts prepared with Different Extracting Methods vol.21, pp.4, 2013, https://doi.org/10.7783/KJMCS.2013.21.4.276
  2. Ginsenoside derivatives and quality characteristics of fermented ginseng using lactic acid bacteria vol.20, pp.4, 2013, https://doi.org/10.11002/kjfp.2013.20.4.573
  3. Effect of High Pressure and Steaming Extraction Processes on Ginsenosides Rg3 and Rh2 Contents of Cultured-Root in Wild Ginseng (Panax ginseng C. A. Meyer) vol.20, pp.4, 2012, https://doi.org/10.7783/KJMCS.2012.20.4.270
  4. Ginsenosides Composition and Antioxidant Activities of Fermented Ginseng Soymilk vol.42, pp.10, 2013, https://doi.org/10.3746/jkfn.2013.42.10.1533
  5. Enhancement of Ginsenosides Conversion Yield by Steaming and Fermentation Process in Low Quality Fresh Ginseng vol.22, pp.3, 2014, https://doi.org/10.7783/KJMCS.2014.22.3.223