Abstract
The 5-year-old seedlings and emblings which regenerated from somatic embryos were compared to the height, DBH, foliar characteristics, content of chlorophyll (chlorophyll a, b and total chlorophyll), carotenoid and leaf microstructure in Liriodendron tulipifera. In comparison of height and DBH (diameter at breast height), no significant differences were found in height (seedling, 3.8 m; embling, 3.87 m) and DBH (seedling, 12.09 cm; embling, 12.53 cm). The emblings and seedlings were similar in values of length (seedling, 108.11 mm, embling, 113.59 mm), width (seedling, 149.1 mm; embling, 167.71 mm), surface area (seedling, $119.92mm^2$; embling, $164.43mm^2$), fresh weight (seedling, 2.1 g; embling, 2.62 g) of leaf, and length (seedling, 81.49 mm; embling, 98.41 mm) and thickness (seedling, 1.66 mm; embling, 1.98 mm) of petiole. In case of chlorophyll content in the leaves, the chlorophyll a (seedlings, $11.2{\mu}g/g$; emblings, $13.2{\mu}g/g$), b (seedlings, $4.8{\mu}g/g$; emblings, $5.4{\mu}g/g$) and total content were higher in emblings ($930.2{\mu}g/g$) than seedlings ($800.1{\mu}g/g$), however, content of carotenoid (seedlings, $260.3{\mu}g/g$; embling, $265.2{\mu}g/g$) showed similar in both plants. Leaves of emblings had a similar pattern of histological structure (palisade or sponge parenchyma) to that of seedlings leaves. Therefore, the results showed that there were no remarkable growth differences when compared with 5-year-emblings and seedlings of yellow poplar.
본 연구는 백합나무 5년생의 실생묘와 체세포배 유래 식물체(클론배양묘, embling) 간의 수고, 흉고직경, 엽 형질, 엽록소 함량 및 엽 조직의 미세구조 비교를 위해 수행되었다. 수고비교의 경우 실생묘의 평균 수고는 3.8 m, 클론배양묘의 경우 3.87 m로 나타나 두 식물체 간에는 거의 차이가 없었고 줄기직경은 실생묘가 12.09 cm, 클론배양묘는 12.53 cm로 나타났지만 큰 차이는 없었다. 엽 특성비교에서는 엽장(실생묘, 108.11 mm; 클론배양묘, 113.59 mm), 엽 폭(실생묘, 149.1 mm; 클론배양묘, 167.71 mm), 엽 면적(실생묘, $119.92mm^2$; 클론배양묘, $164.43mm^2$), 엽 생중량(실생묘, 2.1 g; 클론배양묘, 2.62 g), 엽병 길이(실생묘 81.49 mm; 클론배양묘, 98.41 mm) 및 엽병 두께(실생묘 1.66 mm; 클론배양묘, 1.98 mm)등 클론배양묘가 실생묘보다 모두 높게 나타났다. 또한 엽록소 a(실생묘, $11.2{\mu}g/g$; 클론배양묘, $13.2{\mu}g/g$), 엽록소 b(실생묘, $4.82{\mu}g/g$; 클론배양묘, $5.4{\mu}g/g$) 및 총 엽록소 함량(실생묘, $800.1{\mu}g/g$; 클론배양묘, $930.2{\mu}g/g$) 모두 실생묘 보다 클론배양묘가 높았으나, 카르티노이드 함량(실생묘, $260.3{\mu}g/g$; 클론배양묘, $265.2{\mu}g/g$)은 비슷하게 나타났다. 엽 조직의 미세구조 비교에서는 실생묘 및 클론배양묘 둘 다 책상(palisade)과 해면(sponge) 유조직 각각은 정상적인 배열로 나타났다. 따라서 5년생 클론배양묘와 실생묘의 비교에서 생장차이는 거의 없음을 보여주고 있다.