Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Change of Ripened Persimmon Vinegar with Mountain Ginseng Ingestion on Energy Metabolism in Rats

산양삼 혼입 숙성 감식초 섭취에 의한 흰쥐의 에너지 대사 변화 연구

  • Jeon, Byung-Duk (School of Global Business, Kyungbuk University of Foreign Studies) ;
  • Kim, Pan-Gi (School of Ecological & Environmental Systems, Kyungpook National University) ;
  • Ryu, Sungpil (Department of Leisure Sports, Kyungpook National University)
  • 전병덕 (경북외국어대학교 글로벌비즈니스학부) ;
  • 김판기 (경북대학교 생태환경시스템학부) ;
  • 류승필 (경북대학교 레저스포츠학과)
  • Published : 2012.09.30
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Abstract

In this study, a 4-year-old mountain ginseng was mixed and ripened with 4-year-matured persimmon vinegar, and then it was diluted 5 times and orally administerd to rats. Afterwards, by analyzing the protein expression rate which affects both the carbohydrate metabolism and the lipid metabolism, this study examined the anti-obesity effect of the fusion material. The rats were divided into a control group (CON), a persimmon vinegar group (PV) and a mountain ginseng+persimmon vinegar fusion material group (MPV). The weight gain rate was found to be low in PV and MPV, and the concentration of glucose was also low in PV and MPV. However, GLUT-2 was found to be significantly high in these two groups on the contrary. Both the concentration of free fatty acid and CPT-1 protein expression rate were high in PV and MVP, but MVP was higher than PV. Cytochrome C oxidase was found to be higher in MPV than in CON. AMPK, $PPAR-{\gamma}$ and $PGC1-{\alpha}$ were all high in PV and MPV, but MPV was higher than PV. All the results above verified the thermogenesis effect of the fusion material, leading to an increase of energy metabolism, and it was thought that the fusion material could be effectively used for anti-obesity. However, it seems necessary to verify the anti-obesity effect through various further studies.

본 연구에서는 4년근 산양삼을 4년 숙성된 감식초에 혼입 숙성시킨 후, 5배 희석하여 쥐에게 경구투여하였다. 이후 당질대사 및 지질대사에 영향을 미치는 단백질 발현율을 분석하여 융합소재의 에너지대사율 증대에 의한 비만억제효과에 대하여 검토하였다. 집단은 대조군(CON), 감식초군(PV), 산양삼+감식초 융합소재군(MPV)으로 각각 구분하였다. 체중의 증가율은 PV, MPV가 낮게 나타났다. 글루코스 농도는 PV와 MPV가 낮았으며, 반대로 GLUT-2는 유의하게 높았다. 유리지방산 농도와 CPT-1은 PV, MPV가 높았으며, MPV가 PV에 비하여 높았다. Cytochrome C oxidase는 MPV가 CON에 비하여 높게 나타났다. AMPK, $PPAR-{\gamma}$ 그리고 $PGC1-{\alpha}$는 모두 PV, MPV가 높았으며, MPV가 PV에 비하여 높았다. 이상과 같은 결과는 융합소재의 체열생산반응을 증명하며, 이를 통한 에너지 대사 상승은 비만억제에 효과적으로 사용될 수 있으리라 판단되며, 다양한 후속연구를 통한 검증이 필요할 것이라 사료된다.

Keywords

References

  1. 고성권. 2009. 산양삼과 재배삼의 성분 및 약성 차별화 연구. 국제산삼심포지움 pp.1-9.
  2. 권대근, 강준용, 송영주, 김판기, 서효빈, 류승필. 2012. 산양삼을 첨가한 고지방식이 흰쥐 골격근의 지질과산화 및 항산화 단백질 발현 효과. 한국임학회지 101(1): 69-76.
  3. 김귀란, 윤성란, 이지연, 정용진, 윤경영, 권중호. 2010. 시판 과실식초의 이화학적 품질 및 향기성분 비교. 한국식품저장유통학회 17(5): 616-625.
  4. 김미경, 김미정, 김소연, 정대성, 정용진, 김순동. 1994. 복발효 감식초의 품질. 동아시아식생활학회지 4(2): 45-50.
  5. 류승필, 권태동. 2009. 현미 식초섭취가 중강도 운동 중 에너지대사에 미치는 영향. 한국운동영양학회지 13(3): 217-224.
  6. 류승필, 김철, 배은희. 2012. 산양삼과 감식초의 융합소재를 이용한 비만억제 식품 및 프로그램 개발. 2010 산림과학기술개발사업 연차보고서 p.32.
  7. 박경숙, 이경수, 최영준, 박현숙, 문윤희, 정인철. 2011. 가열 양념 돈육의 품질 및 기호성에 미치는 적포도주 숙성효과. 한국식품조리과학회지 27(3): 95-104.
  8. 배만종. 1991. 인삼 분획성분들이 고지방식이에 의한 비만 유도 Rat에서 간장내 지방축적에 미치는 영향. 한국영양식량학회지 20(1): 27-34.
  9. 서용원, 이혜진, 윤경은, 박혜순. 2009. 중년 비만 여성에서의 에너지 섭취와 휴식 시 에너지 소비 양상. 대한비만학회지 18(1): 31-37.
  10. 서효빈, 송영주, 강준용, 권대근, 김판기, 류승필. 2011. 감식초를 활용한 기능성 음료서의 혈중 지질 농도 감소 와 운동기능성 증대 가능성 검토. 한국임학회지 100(2): 232-239.
  11. 이아롱, 박희동. 2011. 국산 참나무편의 첨가가 적포도 주의 숙성에 미치는 영향. 한국식품저장유통학회지 18(6): 891-897.
  12. 하주헌, 이수호. 2010. 생체 에너지 대사 조절에서 AMPK 의 역할. 대한내분비학회지 25(1): 9-17.
  13. 황유진. 한국산 산양삼의 생리활성에 관한 연구. 미간행 석사학위논문. 성균관대학교.
  14. Bhavsar, S.K., Singh, S., Giri, S., Jain, M.R. and Santani, D.D. 2009. Effect of saponins from Helicteres isora on lipid and glucose metabolism regulating genes expression. Journal of Ethnopharmacology 124(3): 426-433. https://doi.org/10.1016/j.jep.2009.05.041
  15. Bordicchia M, Liu D, Amri EZ, Ailhaud G, Dess-Fulgheri P, Zhang C, Takahashi N, Sarzani R, Collins S. 2012. Cardiac natriuretic peptides act via p38 MAPK to induce the brown fat thermogenic program in mouse and human adipocytes. Journal of Clinical Investigation 122(3): 1022-1036. https://doi.org/10.1172/JCI59701
  16. Clarke, D.C., Miskovic, D., Han, X.X., Calles-Escandon, J., Glatz, J.F., Luiken, J.J., Heikkila, J.J. and Bonen, A. 2004. Overexpression of membrane-associated fatty acid binding protein (FABPpm) in vivo increases fatty acid sarcolemmal transport and metabolism. Physiological Genomics 17(1): 31-37. https://doi.org/10.1152/physiolgenomics.00190.2003
  17. Pujol, E., Rodrguez-Cuenca, S., Frontera, M., Justo, R., Llad, I., Kraemer, F.B., Gianotti, M. and Roca, P. 2004. Regional differences in oxidative capacity of rat white adipose tissue are linked to the mitochondrial content of mature adipocytes. Molecular and Cellular Biochemistry 267(1-2): 157-166.
  18. Eu, C.H., Lim, W.Y., Ton, S.H. and bin Abdul, Kadir. K. 2010. Glycyrrhizic acid improved lipoprotein lipase expression, insulin sensitivity, serum lipid and lipid deposition in high-fat diet-induced obese rats. Lipids Health Disorders 29; 9: 81.
  19. Hattori, M., Kondo, T., Kishi, M. and Yamagami, K. 2010. A single oral administration of acetic acid increased energy expenditure in C57BL/6J mice. Bioscience, Biotechnology and Biochemistry 74(10): 2158-2159. https://doi.org/10.1271/bbb.100486
  20. Hinds, T.D. Jr., Stechschulte, L.A., Cash, H.A., Whisler, D., Banerjee, A., Yong, W., Khuder, S.S., Kaw, M.K., Shou, W., Najjar, S.M. and Sanchez, E.R. 2011. Protein phosphatase 5 mediates lipid metabolism through reciprocal control of glucocorticoid receptor and peroxisome proliferator-activated receptor-$\gamma$(PPAR$\gamma$). Journal of Biological Chemistry 286(50): 42911-42922 https://doi.org/10.1074/jbc.M111.311662
  21. Foretz, M., Ancellin, N., Andreelli, F., Saintillan, Y., Grondin, P., Kahn, A., Thorens, B., Vaulont, S. and Viollet, B. 2005. Short-term overexpression of a constitutively active form of AMP-activated protein kinase in the liver leads to mild hypoglycemia and fatty liver. Diabetes 54(5): 1331-1339. https://doi.org/10.2337/diabetes.54.5.1331
  22. Fushimi, T. and Y. Sato. 2005. Effect of acetic acid feeding on the circadian changes in glycogen and metabolites of glucose and lipid in liver and skeletal muscle of rats. British Journal of Nutrition 94: 714-719. https://doi.org/10.1079/BJN20051545
  23. Fushimi, T., Tayama, K., Fukaya, M., Kitakoshi, K., Nakai, N., Tsukamoto, Y. and Sato, Y. 2001. Acetic acid feeding enhances glycogen repletion in liver and skeletal muscle of rats. Journal of Nutrition 131(7): 1973-1977.
  24. Gu, X., Zhao, H.L., Sui, Y., Guan, J., Chan, J.C. and Tong, P.C. 2011. White rice vinegar improves pancreatic betacell function and fatty liver in streptozotocin-induced diabetic rats. Acta Diabetologica [Epub ahead of print].
  25. Hardie, D.G. 2007. AMP-activated/SNF1 protein kinases: conserved guardians of cellular energy. Nature Reviews Molecular Cell Biology 8: 774-785. https://doi.org/10.1038/nrm2249
  26. Hwang, J.T., Kim, S.H., Lee, M.S., Kim, S.H., Yang, H.J., Kim, M.J., Kim, H.S., Ha, J., Kim, M.S. and Kwon, D.Y. 2007. Anti-obesity effects of ginsenoside Rh2 are associated with the activation of AMPK signaling pathway in 3T3-L1 adipocyte. Biochemical Biophysical Research Communications 364(4): 1002-1008. https://doi.org/10.1016/j.bbrc.2007.10.125
  27. Hwang, J.T., Lee, M.S., Kim, H.J., Sung, M.J., Kim, H.Y., Kim, M.S. and Kwon, D.Y. 2009. Antiobesity effect of ginsenoside Rg3 involves the AMPK and PPAR-gamma signal pathways. Phytotherapy Research 23(2): 262-266. https://doi.org/10.1002/ptr.2606
  28. Jger S, Handschin C, St-Pierre J, Spiegelman BM. 2007. AMP-activated protein kinase (AMPK) action in skeletal muscle via direct phosphorylation of PGC-1alpha. Proceedings of the National Academy of Sciences of the United States of America 104(29): 12017-12022. https://doi.org/10.1073/pnas.0705070104
  29. Jung, H.L., Kwak, H.E., Kim, S.S., Kim, Y.C., Lee, C.D., Byurn, H.K. and Kang, H.Y. 2011. Effects of Panax ginseng supplementation on muscle damage and inflammation after uphill treadmill running in humans. Amercian Journal of Chinese Medicine 39(3): 441-450. https://doi.org/10.1142/S0192415X11008944
  30. Kato, T., Niizuma, S., Inuzuka, Y., Kawashima, T., Okuda, J., Tamaki, Y., Iwanaga, Y., Narazaki, M., Matsuda, T., Soga, T., Kita, T., Kimura, T. and Shioi T. 2010. Analysis of metabolic remodeling in compensated left ventricular hypertrophy and heart failure. Circulation Herat Failure 3(3): 420-430. https://doi.org/10.1161/CIRCHEARTFAILURE.109.888479
  31. Kong X, Wang R, Xue Y, Liu X, Zhang H, Chen Y, Fang F, Chang Y. 2010. Sirtuin 3, a new target of PGC-1alpha, plays an important role in the suppression of ROS and mitochondrial biogenesis. PloS one 5(7): e11707. https://doi.org/10.1371/journal.pone.0011707
  32. Kwon, D.Y., Kim, Y.S., Ryu, S.Y., Choi, Y.H., Cha, M.R., Yang, H.J. and Park, S. 2011. Platyconic acid, a saponin from Platycodi radix, improves glucose homeostasis by enhancing insulin sensitivity in vitro and in vivo. European Journal of Nutrition [Epub ahead of print].
  33. Lai, D.M., Tu, Y.K., Liu, I.M., Chen, P.F. and Cheng, J.T. 2006. Mediation of beta-endorphin by ginsenoside Rh2 to lower plasma glucose in streptozotocin-induced diabetic rats. Planta Medica 72(1): 9-13. https://doi.org/10.1055/s-2005-916177
  34. Lee, M.S., Kim, C.T. and Kim, Y. 2009. Green tea (-)- epigallocatechin-3-gallate reduces body weight with regulation of multiple genes expression in adipose tissue of diet-induced obese mice. Annals of Nutrition & Metabolism 54(2): 151-157. https://doi.org/10.1159/000214834
  35. Liatis, S., Grammatikou, S., Poulia, K.A., Perrea, D., Makrilakis, K., Diakoumopoulou, E. and Katsilambros, N. 2010. Vinegar reduces postprandial hyperglycaemia in patients with type II diabetes when added to a high, but not to a low, glycaemic index meal. European Journal of Clinical Nutrition 64(7): 727-732. https://doi.org/10.1038/ejcn.2010.89
  36. Lin, X., Shim, K. and Odle, J. 2010. Carnitine palmitoyltransferase I control of acetogenesis, the major pathway of fatty acid {beta}-oxidation in liver of neonatal swine. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 298(5): R1435-1443. https://doi.org/10.1152/ajpregu.00634.2009
  37. Moon, Y.J. and Cha, Y.S. 2008. Effects of persimmon-vinegar on lipid metabolism and alcohol clearance in chronic alcohol-fed rats. Journal of Medicinal Food 11(1): 38-45. https://doi.org/10.1089/jmf.2007.071
  38. Morabito, D., Montessuit, C., Rosenblatt-Velin, N., Lerch, R., Vallotton, M.B. and Lang, U. 2002. Impaired glucose metabolism in the heart of obese Zucker rats after treatment with phorbol ester. International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity 26(3): 327-334. https://doi.org/10.1038/sj.ijo.0801881
  39. Nakamura, J. 2010. Phorbol 12-myristate 13-acetate inhibits the antilipolytic action of insulin, probably via the activity of protein kinase $C{\epsilon}$. European Journal of Pharmacology 648(1-3): 188-194. https://doi.org/10.1016/j.ejphar.2010.08.048
  40. Nguyen, K.D., Qiu, Y., Cui, X., Goh, Y.P., Mwangi, J., David, T., Mukundan, L., Brombacher, F., Locksley, R.M. and Chawla, A. 2011. Alternatively activated macrophages produce catecholamines to sustain adaptive thermogenesis. Nature 480(7375): 104-108. https://doi.org/10.1038/nature10653
  41. Ni, H.X., Yu, N.J. and Yang, X.H. 2010. The study of ginsenoside on PPARgamma expression of mononuclear macrophage in type 2 diabetes. Molecular Biology Reports 37(6): 2975-2979. https://doi.org/10.1007/s11033-009-9864-0
  42. Song, Y.J. and Ryu, S. 2010. Studies of the effects of vinegar ingestion after the strenuous Wingate test on energy substrates during recovery periods. Journal of Life Science 20(9): 1345-1352. https://doi.org/10.5352/JLS.2010.20.9.1345
  43. Tisdale, P.B., Seevaratnam, N., Macdonald, I.A., Tsintzas, K. and Bennett, A.J. 2011. Skeletal muscle metabolic gene expression is not affected by dichloroacetate-mediated modulation of substrate utilization. Annals Nutrition & Metabolism 58(1): 19-24. https://doi.org/10.1159/000322971
  44. Tzeng, T.F., Lu, H.J., Liou, S.S., Chang, C.J. and Liu, I.M. 2012. Vinegar-baked Radix Bupleuri regulates lipid disorders via a pathway dependent on peroxisome-proliferator- activated receptor-$\alpha$ in high-fat-diet-induced obese rats. Evidence-based Complementary and Alternative Medicine 2012: 827278.
  45. Yamashita, H., Maruta, H., Jozuka, M., Kimura, R., Iwabuchi, H., Yamato, M., Saito, T., Fujisawa, K., Takahashi, Y., Kimoto, M., Hiemori, M. and Tsuji, H. 2009. Effects of acetate on lipid metabolism in muscles and adipose tissues of type 2 diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Bioscience, Biotechnology and Biochemistry 73(3): 570-576. https://doi.org/10.1271/bbb.80634
  46. Wing, R.R., Goldstein, M.G., Acton, K.J., Birch, L.L., Jakicic, J.M., Sallis, J.F. Jr., Smith-West, D., Jeffery, R.W. and Surwit, R.S. 2001. Behavioral science research in diabetes: lifestyle changes related to obesity, eating behavior, and physical activity. Diabetes Care 24(1): 117-123. https://doi.org/10.2337/diacare.24.1.117
  47. Yamashita, H., Fujisawa, K., Ito, E., Idei, S., Kawaguchi, N., Kimoto, M., Hiemori, M. and Tsuji, H. 2007. Improvement of obesity and glucose tolerance by acetate in type 2 diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Bioscience, Biotechnology, and Biochemistry 71(5): 1236-1243. https://doi.org/10.1271/bbb.60668