Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Effects of Mountain Ginseng-added High Fat diet on Lipid Peroxidation and Antioxidant Protein Expression of Skeletal Muscle in Rats

산양삼을 첨가한 고지방식이 흰쥐 골격근의 지질과산화 및 항산화 단백질 발현 효과

  • Kwon, Dae-Keun (Division of Sports Science, Sunmoon University) ;
  • Kang, Jun-Yong (Division of Sports Science, Sunmoon University) ;
  • Song, Youngju (Division of Sports Science, Sunmoon University) ;
  • Kim, Pan-Gi (School of Ecological & Environmental Systems, Kyungpook National University) ;
  • Seo, Hyobin (Department of Leisure Sports, Kyungpook National University) ;
  • Ryu, Sungpil (Department of Leisure Sports, Kyungpook National University)
  • 권대근 (선문대학교 스포츠과학부) ;
  • 강준용 (선문대학교 스포츠과학부) ;
  • 송영주 (선문대학교 스포츠과학부) ;
  • 김판기 (경북대학교 생태환경시스템학부) ;
  • 서효빈 (경북대학교 레저스포츠학과) ;
  • 류승필 (경북대학교 레저스포츠학과)
  • Published : 2012.03.31
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Abstract

We investigated the effect of mountain ginseng-added high fat diet supplementation on lipid peroxidation and antioxidant protein expressions in rats. Thirty-two male rats were randomly divided into 4 groups; HS (high-fat diet sedentary group, n=8), MG1 (0.5% mountain ginseng-added diet group, n=8), MG2 (1% mountain ginsengadded diet group, n=8) and MG3 (2% mountain ginseng-added diet group, n=8). They have fed the diet for 4 weeks. The blood triglyceride were significantly lower in the MG1 and MG2 groups than that of the HS group. The blood HDL-cholesterol were significantly higher in the MG3 group than that of the HS and MG2 groups. The muscle glycogen contents of the MG2 and MG3 groups were significantly higher than that of HS and MG1 groups. The MDA contents in the MG1, MG2 and MG3 groups tended to lower than the HS group. The GPx protein expression in the gastrocnemius muscle of the MG2 group was significantly increased compared to that of the HS group. The Cu,Zn-SOD protein expression in the gastrocnemius muscle of the MG1 and MG2 groups was significantly increased compared to that of the MG3 group. The Mn-SOD protein expression in the MG1, MG2 and MG3 groups tended to higher than the HS group. From these results, it was suggested that mountain ginseng-added diet may have an crucial role on decreased MDA levels and increased antioxidant function in the skeletal muscle of rat fed a high fat diet.

본 연구에서는 산양삼을 첨가한 고지방식이를 하였을 때 흰쥐 골격근의 지질과산화와 항산화 단백질의 발현 정도를 분석하고자 하였다. 32마리의 웅성 흰쥐를 대상으로 난괴법을 사용하여 HS(고지방식이 대조군), MG1(0.5%의 산양삼 첨가식이 섭취군), MG2(1.0%의 산양삼 첨가식이 섭취군), 그리고 MG3(2.0%의 산양삼 첨가식이 섭취군)으로 각각 8마리씩 분류하였다. 식이는 4주간 섭취시켰다. 혈중 중성지방은 MG1과 MG2가 HS에 비하여 유의하게 낮았으며, 혈중 HDL-C는 MG3이 HS 및 MG2에 비하여 높게 나타났다. 근육 글리코겐 함량은 MG2, MG3이 HS와 MG1에 비하여 높았으며, MDA 함량은 MG1, MG2, MG3이 HS에 비하여 낮은 경향만이 나타났다. 비복근의 GPx 단백질 발현은 MG2가 HS에 비하여 높았으며, Cu,Zn-SOD은 MG1과 MG2가 높게 나타났다. Mn-SOD는 MG1, MG2, MG3이 HS에 비하여 높은 경향만이 나타났다. 이상의 결과, 고지방식이에 산양삼 첨가는 골격근의 MDA 함량을 저하시키고 항산화기능을 증가시키는 중요한 역할을 한다고 할 수 있다.

Keywords

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