The Korean journal of internal medicine

The Korean Association of Internal Medicine (대한내과학회)
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DNA Methylation of RUNX3 in Papillary Thyroid Cancer

  • Ko, Hee Ja (Department of Internal Medicine, Soonchunhyang University College of Medicine) ;
  • Kim, Bo Yeon (Department of Internal Medicine, Soonchunhyang University College of Medicine) ;
  • Jung, Chan Hee (Department of Internal Medicine, Soonchunhyang University College of Medicine) ;
  • Chun, Sung Wan (Department of Internal Medicine, Soonchunhyang University College of Medicine) ;
  • Mok, Ji Oh (Department of Internal Medicine, Soonchunhyang University College of Medicine) ;
  • Kim, Yeo Joo (Department of Internal Medicine, Soonchunhyang University College of Medicine) ;
  • Park, Hyeong Kyu (Department of Internal Medicine, Soonchunhyang University College of Medicine) ;
  • Kim, Chul Hee (Department of Internal Medicine, Soonchunhyang University College of Medicine) ;
  • Kim, Sang Jin (Department of Internal Medicine, Soonchunhyang University College of Medicine) ;
  • Byun, Dong Won (Department of Internal Medicine, Soonchunhyang University College of Medicine) ;
  • Suh, Kyo Il (Department of Internal Medicine, Soonchunhyang University College of Medicine) ;
  • Yoo, Myung Hi (Department of Internal Medicine, Soonchunhyang University College of Medicine) ;
  • Kang, Sung Gu (Department of Internal Medicine, Soonchunhyang University College of Medicine)
  • Published : 2012.12.01
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Abstract

Background/Aims: The relationship between Runt-related transcription factor 3 (RUNX3) gene inactivation and various solid tumors has been reported; however, little information is available about RUNX3 in thyroid cancers. Methods: We evaluated the DNA methylation of RUNX3 in 13 papillary thyroid cancer tissues and four thyroid cancer cell lines. Additionally, using reverse transcriptase-polymerase chain reaction, we analyzed RUNX3 gene expression in several thyroid cancer cell lines after treating with the demethylating agent 5-aza-2'-deoxycytidine (DAC). Results: RUNX3 was hypermethylated in many thyroid cancer cell lines and in 10 of the 12 papillary thyroid cancer tissues. Treatment with DAC increased the expression of RUNX3 in some thyroid cancer cell lines. Conclusions: We suggest that RUNX3 is associated with thyroid carcinogenesis, and RUNX3 methylation is a potentially useful diagnostic marker for papillary thyroid cancer.

Keywords

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