Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Fluorescently Labeled Nanoparticles Enable the Detection of Stem Cell-Derived Hepatocytes

  • Ha, Young-Eun (Department of Bioengineering, College of Engineering, Hanyang University) ;
  • Shin, Jin-Sup (Department of Bioengineering, College of Engineering, Hanyang University) ;
  • Lee, Dong-Yun (Department of Bioengineering, College of Engineering, Hanyang University) ;
  • Rhim, Tai-Youn (Institute for Bioengineering and Biopharmaceutical Research, Hanyang University)
  • Received : 2012.03.09
  • Accepted : 2012.03.19
  • Published : 2012.06.20
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Abstract

Stem cell transplantation is emerging as a possible new treatment for liver cirrhosis, and recent animal studies have documented the benefits of stem cell therapy in a hepatic fibrosis model. However, the underlying mechanism of stem cell therapy is still unclear. Among the proposed mechanisms, the cell replacement mechanism is the oldest and most important, in which permanently damaged tissue can be replaced by normal tissue to restore function. In the present study, Cy5.5-labeled superparamagnetic iron oxide (SPIO) was used to label human mesenchymal stem cells. The uptake of fluorescently labeled nanoparticles enabled the detection and monitoring of the transplanted stem cells; therefore, we confirmed the direct incorporation and differentiation of SPIO into the hepatocyte-like transplanted stem cells by detecting human tyrosine aminotransferase (TAT), well-known enzymatic marker for hepatocyte-specific differentiation.

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