Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Molecular Imaging of Arthritis in the Angiogenic Vasculature Using A 123I-Vascular Endothelial Growth Factor Receptor Antibody

  • Kim, Sung-Min (Division of Magnetic Resonance Research, Korea Basic Science Institute) ;
  • Choi, Na-Eun (Division of Magnetic Resonance Research, Korea Basic Science Institute) ;
  • Song, Young-Kyu (Division of Magnetic Resonance Research, Korea Basic Science Institute) ;
  • Cho, Gyung-Goo (Division of Magnetic Resonance Research, Korea Basic Science Institute) ;
  • Bang, Jeong-Kyu (Division of Magnetic Resonance Research, Korea Basic Science Institute) ;
  • Kim, Sang-Mi (Department of Radiology and Research Institute of Radiology, University of Ulsan College of Medicine, Asan Medical Center) ;
  • Lee, Sang-Hoon (Department of Radiology and Research Institute of Radiology, University of Ulsan College of Medicine, Asan Medical Center) ;
  • Ryu, Eun-Kyoung (Division of Magnetic Resonance Research, Korea Basic Science Institute)
  • Received : 2012.01.26
  • Accepted : 2012.03.05
  • Published : 2012.06.20
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Abstract

Vascular endothelial growth factor (VEGF) and its receptor (VEGFR) have been implicated in the pathogenesis of rheumatoid arthritis, which is angiogenesis dependent. Antibody-based molecular imaging improves targeting, and antibody radiolabeling is useful for monitoring biological events $in$ $vivo$ $via$ PET or SPECT. We investigated the potential of molecular imaging to diagnose arthritis with VEGFR-2 $in$ $vivo$. The $^{123}I$-VEGFR-2 antibody was prepared by the iodogen tube method. The radioligand was injected into arthritic mice, and micro SPECT/CT was performed. The arthritic mice were examined by 4.7-T MRI and immunohistochemistry. The $^{123}I$-VEGFR-2 antibody showed high uptake in the arthritic region at 1 h postinjection on SPECT/CT but no uptake in the control animals after radioligand injection. In MR images, the arthritic tissue of the mice was correlated with regions labeled by the $^{123}I$-VEGFR-2 antibody. Immunohistochemical localization showed markedly increased expression of VEGFR-2 in the endothelial cells, fibroblasts, and macrophages of the arthritic mice.

Keywords

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