Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Identification of Anti-obesity Constituents from Yukeuigambitang

육의감비탕(肉薏減肥湯)의 항비만 효능 및 유효성분 규명

  • Wang, Shian (Department of Oriental Medical Food and Nutrition, Semyung University) ;
  • Song, Hyo-Nam (Department of Oriental Medical Food and Nutrition, Semyung University) ;
  • Choi, Won-Ik (Department of Oriental Medical Food and Nutrition, Semyung University) ;
  • Park, Jong-Hyuk (College of Bionano Technology, Gachon University) ;
  • Jeong, Youg-Joon (College of Bionano Technology, Gachon University) ;
  • Kang, Se-Chan (College of Bionano Technology, Gachon University) ;
  • Ko, Sung-Kwon (Department of Oriental Medical Food and Nutrition, Semyung University) ;
  • Bhang, Dae-Hyuk (Ssial Food Inc.)
  • 왕사한 (세명대학교 한방식품영양학부) ;
  • 송효남 (세명대학교 한방식품영양학부) ;
  • 최원익 (세명대학교 한방식품영양학부) ;
  • 박종혁 (가천대학교 바이오나노대학) ;
  • 정용준 (가천대학교 바이오나노대학) ;
  • 강세찬 (가천대학교 바이오나노대학) ;
  • 고성권 (세명대학교 한방식품영양학부) ;
  • 방대혁 ((주)씨알푸드)
  • Received : 2012.01.04
  • Accepted : 2012.04.23
  • Published : 2012.04.30
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Abstract

To develop antiobese food materials from medicinal plants, isolation of antiobese active compounds in $Yukeuigambitang$ of which activity was already proved in the previous study by animal experiments was performed. Antiobese effect of stepwise solvent fractions from 70% ethanol extract of $Yukeuigambitang$ was determined by the differentiation inhibition activity on 3T3-L1 preadipocytes. CH2Cl2 fraction had significant antiobese activity, and n-Hexane and EtOAc fractions were the next. Three phenolic compounds from $CH_2Cl_2$ fraction were identified by GC/MS analysis and one compound was finally isolated by HPLC. It was revealed as a new compound presumed to be one of the derivatives produced from the medicinal plants mixture in $Yukeuigambitang$.

식품의 원료로 사용가능한 안전한 생약자원 유래의 항비만 소재 개발을 위하여 추출물 단계에서 3T3-L1 전지방세포의 지방세포로의 분화 억제 활성 효과를 나타낸 육의감비탕의 생리활성 성분을 규명하고자 하였다. 70% EtOH로 가열추출 후 계통학적인 용매분획법에 의해 분획한 분획물을 대상으로 3T3-L1 세포의 지방분해 억제시험을 실시한 결과 $CH_2Cl_2$ 분획물에서 가장 우수한 활성이 나타났으며, n-Hexane 분획물과 EtOAc 분획물에서도 약한 지방세포 분화억제 효능이 관찰되었다. 이중 $CH_2Cl_2$ 분획물로부터 각종 chromatography 기법을 통하여 분석한 결과 3종의 phenol류 화합물을 GC-mass 화합물사전 검색을 통해 동정 할 수 있었으며 이 중 1종의 화합물을 HPLC를 이용하여 단리하였다. 단리된 화합물은 육의감비탕을 열추출하는 과정에서 구성하고 있는 생약 중 육계로부터 유래된 유도체인 것으로 추측되며 따라서, 육의감비탕에 포함되어 있는 육계로부터 육의감비탕 조제시 생성되는 물질로 판단된다. 육의감비탕은 선행연구에서 처음으로 개발된 처방으로 본 연구를 통해 단리된 화합물외에도 항비만 효과를 지닌 유사한 화합물들이 존재할 것으로 사료되어 향후 기능성식품의 소재로서 안전하게 사용할 수 있을 것으로 기대된다.

Keywords

References

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