Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Downstream Networking of $Zap70$ in Meiotic Cell Cycle of the Mouse Oocytes

  • Kim, Hyun-Jung (Department of Biomedical Science, College of Life Science, CHA University CHA Research Institute, Fertility Center, CHA General Hospital) ;
  • Lee, Hyun-Seo (Department of Biomedical Science, College of Life Science, CHA University CHA Research Institute, Fertility Center, CHA General Hospital) ;
  • Kim, Eun-Young (Department of Biomedical Science, College of Life Science, CHA University CHA Research Institute, Fertility Center, CHA General Hospital) ;
  • Lee, Kyung-Ah (Department of Biomedical Science, College of Life Science, CHA University CHA Research Institute, Fertility Center, CHA General Hospital)
  • Received : 2012.02.25
  • Accepted : 2012.03.19
  • Published : 2012.03.31
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Abstract

Previously, we found that $Zap70$ (Zeta-chain-associated protein kinase) expressed in the mouse oocytes and played significant role in completion of meiosis specifically at MI-MII (metaphase I-II) transition. Microinjection of $Zap70$ dsRNA into the cytoplasm of germinal vesicle oocyte resulted in MI arrest, and exhibited abnormalities in their spindles and chromosome configurations. The purpose of this study was to determine the mechanisms of action of $Zap70$ in oocyte maturation by evaluating downstream signal networking after $Zap70$ RNAi (RNA interference). The probe hybridization and data analysis were used by Affymetrix Gene Chip Mouse Genome 430 2.0 array and GenPlex 3.0 (ISTECH, Korea) software, respectively. Total 1,152 genes were up (n=366) and down (n=786) regulated after $Zap70$ RNAi. Among those genes changed, we confirmed the expressional changes of the genes involved in the regulation of actin cytoskeleton and MAPK (mitogen-activated protein kinase) signaling pathway, since the phenotypes of $Zap70$ RNAi in oocytes were found in the changes in the chromosome separation and spindle structures. We confirmed the changes in gene expression in the actin skeletal system as well as in the MAPK signaling pathway, and concluded that these changes are main cause of the aberrant chromosome arrangement and abnormal spindles after $Zap70$ RNAi.

Keywords

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