약학회지 (YAKHAK HOEJI)

  • 제56권6호
  • /
  • Pages.382-389
  • /
  • 2012
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  • 0377-9556(pISSN)
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  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

랫드의 혈액응고 및 혈소판 응집에 미치는 은나노 입자의 영향

Effects of Citrate-capped Silver Nanoparticles on the Blood Coagulation and Platelet Aggregation in Rats

  • 이연진 (동덕여자대학교 약학대학) ;
  • 박광식 (동덕여자대학교 약학대학)
  • Lee, Yeonjin (College of Pharmacy, Dongduk Women's University) ;
  • Park, Kwangsik (College of Pharmacy, Dongduk Women's University)
  • 투고 : 2012.11.12
  • 심사 : 2012.12.27
  • 발행 : 2012.12.31
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초록

Effects of citrate-capped silver nanoparticles (AgNPs) on the blood coagulation and platelet aggregation were investigated using whole blood, platelet rich plasma (PRP) and washed platelet obtained from SD male rats. To confirm the stability of AgNPs in the test, size distribution of the nanoparticles was measured in the vehicles including distilled water, serum, and platelet buffers. The average size of AgNPs was 20 nm in the vehicles, which means that the stability was maintained during the whole experimental period. When blood coagulation was monitored by using whole blood impedance aggregometer, coagulation was not observed at the concentration of 1, 10 and 50 ppm. Platelets in plasma or in buffer were not aggregated by AgNPs at the concentration of 1, 2 and 4 ppm, respectively. The test concentration of AgNPs could not be increased because the dark color of the nanoparticles impeded the transmission of light, which is an indicator of aggregation. Although the blood or platelets were pre-activated by collagen, thrombin, or ADP with sub-threshold level, aggregation was not observed at the test concentration. Microscopic observation also supported the result obtained by the aggregometer.

키워드

과제정보

연구 과제 주관 기관 : 동덕여자대학교

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