Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Antidiabetic Effects of Water Extract from Taraxacum coreanum Nakai in type II Diabetic Mice

포공영(浦公英) 물 추출물의 항당뇨 효과에 대한 연구

  • Oh, Chan Ho (Department of Food Science & Biotechnology, College of Food Science, Woosuk University) ;
  • Shin, Nam Sik (Nimmaeul Corporation LTD.) ;
  • Kwan, Jin (Department of Prosthetics & Orthotics, Korea National College of Rehabilitation & Welfare) ;
  • Lee, Kwang Gyu (Department of Oriental Pathology, College of Oriental Medicine, Woosuk University) ;
  • Lee, Sang Ryong (Department of Meridian & Acupoint, College of Oriental Medicine, Woosuk University) ;
  • Lee, Chang Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
  • 오찬호 (우석대학교 식품과학대학 식품생명공학과) ;
  • 신남식 (님마을 민들레 영농조합) ;
  • 권진 (한국재활복지대학 의료보장구과) ;
  • 이광규 (우석대학교 한의과대학 한방병리학교실) ;
  • 이상룡 (우석대학교 한의과대학 경혈학교실) ;
  • 이창현 (우석대학교 한의과대학 해부학교실)
  • Received : 2012.08.31
  • Accepted : 2012.10.10
  • Published : 2012.10.25
Download PDF
⟨ Previous Next ⟩

Abstract

Taraxacum coreanum Nakai (TM) has been investigated for its antidiabetic activity in streptozotocin induced diabetic animals, but few studies have been conducted to evaluate its efficacy for treatment of type II diabetic mice. This study was designed to evaluate the efficacy of the water extracts of TM for the treatment of type II diabetes. Twenty four mice(C57BLKS/J Iar $-+Lepr^{db}/+Lepr^{db}$) with type II diabetes were randomly assigned either to a experimental group A (leaves extraction of TM), experimental group B(roots extraction of TM), experimental group C(leaves, roots and flowers extraction of TM) or control group. Water extracts of TM were ingested for 10 weeks. The changes of body weight, level of blood glucose, numerical change of insulin-immunoreactive cells and level of blood insulin were measured after treatment of water extracts of TM. A reduction of the blood glucose level after treatment of TM was observed in all the experimental groups. Especially, experimental C group showed significant reduction of blood glucose level compared with control group. Experimental C group induced an increase the level of blood insulin and density of insulin immunoreactive cells after treatment of TM. These results show that water extracts of TM can improve the severity in type II diabetic mice.

Keywords

Acknowledgement

Supported by : 우석대학교

References

  1. 전국한의과대학 본초학교수 편. 본초학, 서울, 영림사, pp 243-245, 2007.
  2. 중약대사전 편찬위원회. 中藥大辭典, 정담, pp 4553-4558, 1999.
  3. 주영승. 운곡본초학, 서울, 서림재, pp 205-208, 2004.
  4. 王本祥. 現代中葯葯理與臨床, 天津科技翻譯出版公司, pp 1594-1596, 2004.
  5. 박지윤, 장주연, 이미경, 박은미, 김명주, 손동화, 정현채, 조수열. 민들레 추출물이 당뇨유발 흰쥐의 장내 미생물 균총에 미치는 영향. 한국식품영양과학회지 31: 1112-1118, 2002.
  6. 김명주, 조수열. 서양민들레가 streptozotocin으로 유발한 당뇨 흰쥐의 뇌조직중 유해 활성산소 생성 및 제거 효소계에 미치는 영향. 한국식품영양과학회지 31: 500-505, 2002.
  7. 김지현, 이혜인, 박주희, 임윤영, 김범준, 임인석, 김명남, 김현석, 김종근, 한성훈, 조수묵, 김재현, 박기문. 아토피피부염 환자에서 민들레 추출물 함유제(AF-343)의 효과에 관한 연구. 천식 및 알레르기 30: 36-42, 2010.
  8. Mascolo, N., Autore. G., Capasso, F., Menghini, A., Fasulo M.P. Biological screening of Italian medicinal plants for anti-inflammatory activity. Phytother Res 1: 28-31, 1974.
  9. Cristinf, A.W., Fiona, G., Jenny, G. Flavonoids, cinnamic acids and courmarins from the different tissue and medicinal preparations of Taraxacum officinale. Phytochemistry 42: 121-127, 1996. https://doi.org/10.1016/0031-9422(95)00865-9
  10. 조수열, 박지윤, 오연진, 장주연, 박은미, 김명주, 김광수. 민들레잎 추출물의 흰쥐 체내 지질대사 개선효과. 한국식품영양과학회지 29: 676-682, 2000.
  11. Hsu, S.M., Raine, L., Fanger, H. Use of avidin-biotinperoxidase complex(ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody(PAP) procedures. J Histochem Cytochem 29: 577-580, 1981. https://doi.org/10.1177/29.4.6166661
  12. Nakano, T., Araki, K., Nakatani, H., Kobayashi, M., Sugimoto, T., Furuya, Y., Matsuoka, T., Jin, T., Hanazaki, K. Effects of geldanamycin and thalidomide on the Th1/Th2 cytokine balance in mice subjected to operative trauma. Surgery 141: 490-500, 2007. https://doi.org/10.1016/j.surg.2006.10.003
  13. Zhang, C., Zou, X., Peng, J., Xiang, M. Immune tolerance induced by adoptive transfer of dendritic cells in an insulin-dependent diabetes mellitus murine model. Acta Pharmacol Sin 28: 98-104, 2007. https://doi.org/10.1111/j.1745-7254.2007.00467.x
  14. Kim, H.M., Shin, H.Y., Lim, K.H., Ryu, S.T., Shin, T.Y., Chae, H.J., Kim, H.R., Lyu, Y.S., An, N.H., Lim, K.S. Taraxacum officinale inhibits tumor necrosis factor-alpha production from rat astrocytes. Immunopharmacol Immunotoxicol 22: 519-530, 2000. https://doi.org/10.3109/08923970009026009
  15. Shen, Q., Gu, G.P., Wu, G.R., Zhang, W.M. Study on the Progress of the Dandelion. The Chinese Academic Medical Magazine of Organisms 2: 6-7, 2004.
  16. Lin, Y., Zheng, J.H. Research progress of a Chinese traditional drug taraxacum herb. The Chinese Journal of Modern Applied Pharmacy 15: 42-47, 1998.
  17. Hu, C., Kitts, D.D. Antioxidant, prooxidant, and cytotoxic activities of solvent-fractionated dandelion (Taraxacum officinale) flower extracts in vitro. J Agric Food Chem 51: 301-310, 2003. https://doi.org/10.1021/jf0258858
  18. Jeon, H.J., Kang, H..J, Jung, H.J., Kang, Y.S., Lim, C.J., Kim, Y.M., Park, E.H. Anti-inflammatory activity of Taraxacum officinale. J Ethnopharmacol 115: 82-88, 2008. https://doi.org/10.1016/j.jep.2007.09.006
  19. Schutz, K., Carle, R., Schieber, A. Taraxacum-a review on its phytochemical and pharmacological profile. J Ethnopharmacol 107: 313-323, 2006. https://doi.org/10.1016/j.jep.2006.07.021
  20. Williams, C.A., Goldstone, F., Greeham, J. Flavonoids, cinnamic acids and coumarins from the different tissues and medicinal preparations of Taraxacum officinale. Phytochemistry 42: 121-127, 2008.
  21. Schutz, K., Kammerer, D.R., Carle, R., Schieber, A. Characterization of phenolic acids and flavonoids in dandelion (Taraxacum officinale WEB. ex WIGG.) root and herb by high-performance liquid chromatography/ electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom 19: 179-186, 2005. https://doi.org/10.1002/rcm.1767
  22. Rodriguez-Fragoso, L., Reyes-Esparza, J., Burchiel, S.W., Herrera-Ruiz, D., Torres, E. Risks and benefits of commonly used herbal medicines in Mexico. Toxicol Appl Pharmacol 227: 125-135, 2008. https://doi.org/10.1016/j.taap.2007.10.005
  23. British Herbal Medicine Association, "Dandelion Leaf"and "Dandelion Root" vol. 1. British Herbal Pharmacopoeia, pp 37-39, 1990.
  24. ESCOP. "Taraxaci folium"and "Taraxaci radix" Monographs on the Medicinal Uses of Plant Drugs. European Scientific Cooperative on Phytotherapy, 2nd ed. Thieme, Stuttgart, pp 499-504, 2003.
  25. Blumenthal, M., Busse, W.R., Goldberg, A., Gruenwald, J., Hall, T., Riggins, C.W., Rister, R.S. (Eds.). "Dandelion herb"and "Dandelion root with herb"in: The complete german commission E Monographs. Therapeutic guide to herbal medicines. American Botanical Council, Austin, Texas, pp 118-120, 1998.
  26. Institute for animal reproduction[(재)동물번식연구소 (WWW.iar.or.jp)], 당뇨병마우스(db마우스), Japan.
  27. Hernandez-Galicia, E., Aguilar-Contreras, A., Aguilar- Santamaria, L., Roman-Ramos, R., Chavez-Miranda, A.A., Garcia-Vega, L.M., Flores-Saenz, J.L., Alarcon-Aguilar, F.J. Studies on hyperglycemic activity of Mexican medicinal plants. In: Proceedings of the Western Pharmacology Society 45: 118-124, 2002.
  28. Onal, S. Timur, S., Okutucu, B., Zihnioglu F. Inhibition of alpha-glucosidase by aqueous extracts of some potent antidiabetic medicinal herbs. Prep Biochem Biotechnol 35: 29-36, 2005. https://doi.org/10.1081/PB-200041438
  29. Ertas, O.S., Aktas, H.F., Haznedaroglu, M.Z. Analysis of sodium and potassium levels in Taraxacum officinale by flame emission photometry. Acta Pharmaceutica Turcica 47: 127-130, 2005.
  30. Petlevski, R., Hadzija, M., Slijepcevic, M., Juretic, D., 2001. Effect of 'ntidiabetis' herbal preparation on serum glucose and fructosamine in NOD mice. J Ethnopharmacol 75: 181-184, 2001. https://doi.org/10.1016/S0378-8741(01)00177-5
  31. Akhtar, M.S., Khan, Q.M., Khaliq, T. Effects of Portulaca oleracae(kulfa) and Taraxacum officinale (dhudhal) in normoglycaemic and alloxan-treated hyperglycaemic rabbits. J Pak Med Assoc 35: 207-210, 1985.
  32. Hussain, Z., Waheed, A., Qureshi, R.A., Burdi, D.K., Verspohl, E.J., Khan, N., Hasan, M. The effect of medicinal plants of Islamabad and Muree region of Pakistan on insulin secretion from INS-1 cells. Phytother Res 18: 73-77, 2004. https://doi.org/10.1002/ptr.1372
  33. Petlevski, R., Hadzija, M., Slijepcevic, M., Juretic, D., Petrik, J., 2003. Glutathione S-transferase and malondialdehyde in the liver of NOD mice on short-term treatment with plant mixture extract P-980191. Phytother Res 17: 311-314, 2003. https://doi.org/10.1002/ptr.1128
  34. Cho, S.Y., Park, J.Y., Park, E.M., Choi, M.S., Lee, M.K., Jeon, S.M., Jang, M.K., Kim, M.J., Park, Y.B. Alternation of hepatic antioxidant enzyme activities and lipid profile in streptozotocin-induced diabetic rats by supplementation of dandelion water extract. Clin Chim Acta 317: 109-117, 2002. https://doi.org/10.1016/S0009-8981(01)00762-8
  35. Semsei, I., Rao, G., Richardson, A. Expression of superoxide dismutase and catalase in rat brain as a function of age. Mech ageing Devel 58: 13-19, 1991. https://doi.org/10.1016/0047-6374(91)90116-H
  36. Mooradian, A.D., Dickerson, F., Smith, T.L., Lipid order and composition of synaptic membranes in experimental diabetes mellitus. Neurochem Res 15: 981-985, 1990. https://doi.org/10.1007/BF00965743
  37. Goksu, E., Eken, C., Karadeniz, O., Kucukyilmaz, O. First report of hypoglycemia secondary to dandelion (Taraxacum officinale) ingestion. Am J Emerg Med 28(1):111.e1-e2, 2010. https://doi.org/10.1016/j.ajem.2009.02.021
  38. 方樂中. 실용중의내과학, 상해 : 상해과학기술출판사, p 477, 1986.