Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Cytogenetic Analysis of Reciprocal Hybrids Reveals a Robertsonian Translocation between Mud Loach ($Misgurnus$ $mizolepis$) and Cyprinid Loach ($M.$ $anguillicaudatus$)

미꾸라지($Misgurnus$ $mizolepis$)와 미꾸리($M.$ $anguillicaudatus$) 및 유도된 종간 잡종의 세포유전학적 연구

  • Lee, Seung-Ki (National Institute of Biological Resources) ;
  • Kim, Dong-Soo (Department of Aquaculture/Institute of Marine Living Modified Organisms (iMLMO), Pukyong National University)
  • 이승기 (국립생물자원관) ;
  • 김동수 (부경대학교 해양바이오신소재학과)
  • Received : 2012.01.30
  • Accepted : 2012.03.26
  • Published : 2012.03.31
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Abstract

Reciprocal hybrids between the mud loach ($Misgurnus$ $mizolepis$) and cyprinid loach ($M.$ $anguillicaudatus$) were produced by artificial fertilization. The chromosome number of mud loach was 2n=48, consisting of 12M+4SM+32A chromosomes. The cyprinid loach has 2n=50, consisting of 10M+4SM+36A chromosomes. The chromosome numbers of the diploid reciprocal hybrids were 2n=49, consisting of 11M+4SM+34A chromosomes. All the karyotypes documented in this study had the same arm number of 64. There was no evidence of chromosomal polymorphisms or sex-related heteromorphism. The cytogenetic traits of the hybrid genotypes were intermediate between those of the parent species. In all genotypes, the chromosomal NORs localized to the terminal short arms of the same metacentric chromosome pair. These results suggest that Robertsonian translocation occurred between metacentric chromosome 1 of mud loach and acrocentric chromosome of cyprinid loach.

미꾸라지, 미꾸리 및 상반교배를 통해 유도된 종간 잡종의 세포유전학적 분석을 수행하였다. 미꾸라지와 미꾸리의 염색체 수는 각각 2n=48 (12M+4SM+32A), 2n=50 (10M+4SM+36A)이었고, 잡종군들의 염색체수는 각각 2n=49 (11M+4SM+34A)였다. 모든 그룹의 염색체는 동일한 arm number (NF=64)를 갖고 있었으며, 염색체 다형현상, 암수 간 이형의 염색체는 관찰되지 않았다. 적혈구의 크기, DNA 함량을 분석한 결과 잡종군들은 미꾸라지와 미꾸리의 중간 값을 나타냈다. 염색체의 NORs (nucleolar organizing regions)은 모두 동일한 중부염색체 단완부에서 Ag-positive signal이 나타났다. 이상의 결과는 미꾸라지의 1번 중부 염색체와 미꾸리의 차단부 염색체가 Robertsonian 형의 염색체 전좌 과정을 거쳤을 것을 시사한다.

Keywords

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