The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effect of Ssanghwa-tang Extract on Antioxidant and Anti-aging Enzyme Activities

쌍화탕 추출물이 항산화효소 및 항노화관련 효소 활성에 미치는 영향

  • Park, Ji-Young (Department of Herbal Pharmacology, College of Oriental Medicine & R&D Team for the New Drug of Oriental Medicine (BK21), Daegu Haany University) ;
  • Hwang, Jae-Gyu (New Drug Development Center, Daegu Gyeongbuk High-tech Medical Industry Foundation) ;
  • Yun, Jong-Kuk (Daegu Technopark Oriental Medicine Industry Support Center) ;
  • Han, Kil-Hwan (Daegu Technopark Oriental Medicine Industry Support Center) ;
  • Do, Eun-Ju (Daegu Technopark Oriental Medicine Industry Support Center) ;
  • Kim, Sung-Ok (Department of Herbal Pharmacology, College of Oriental Medicine & R&D Team for the New Drug of Oriental Medicine (BK21), Daegu Haany University) ;
  • Kim, Mi-Ryeo (Department of Herbal Pharmacology, College of Oriental Medicine & R&D Team for the New Drug of Oriental Medicine (BK21), Daegu Haany University)
  • 박지영 (대구한의대학교 한의과대학 본초약리학교실 & BK21 한방신약개발연구팀) ;
  • 황재규 (대구경북첨단의료산업진흥재단 신약개발지원센터) ;
  • 윤종국 ((재)대구테크노파크 한방산업지원센터) ;
  • 한길환 ((재)대구테크노파크 한방산업지원센터) ;
  • 도은주 ((재)대구테크노파크 한방산업지원센터) ;
  • 김성옥 (대구한의대학교 한의과대학 본초약리학교실 & BK21 한방신약개발연구팀) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실 & BK21 한방신약개발연구팀)
  • Received : 2012.04.18
  • Accepted : 2012.04.28
  • Published : 2012.05.30
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Abstract

Objectives : The present study was designed to investigate effects of Ssanghwa-tang (Shu$\bar{a}$nghu$\bar{a}$-g$\bar{e}$ng) on oxidation/reduction reaction-related and aging-related enzymes $in$ $vitro$. Methods : We performed MTT assay, collagenase inhibition assay, elastase inhibition assay, tyrosinase inhibition assay, DPPH free radical scavenging assay, SOD-like activity and xanthine oxidase (XO) inhibition assay. Results : The 50% ethanol (EtOH) extract of Ssanghwa-tang (SHT) showed 55% inhibition of collagenase activity, and 42% inhibition of elastase activity at 1 mg/ml concentration. Also it's treatment showed 18% inhibition of tyrosinase activity, to relate whitening effect, at the same dose of 50% ethanol extract of SHT. Antioxidant activities were determined by DPPH radical scavenging, XO inhibiting activity and SOD-like activity. These scavenging, XO-inhibiting and SOD-like activities were measured in 80%, 75%, and 28% inhibitions, respectively, at a 1 mg/ml treated dose, compared to those of control. The inhibitory effects of 50% EtOH extract on aging and oxidation-related enzyme activities were higher than those of water extract and 95% EtOH extract. Conclusions : Taken together, our findings suggest that the SHT has potential and applicable benefits for development of cosmetics to have anti-aging (anti-wrinkle and whitening) and anti-oxidation functions.

Keywords

References

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