The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Genetic Differentiation of Pseudomonas syringae Pathovar tomato from Other P. syringae Pathovars using REP-PCR and URP-PCR

  • Cho, Min-Seok (National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Dong-Suk (National Academy of Agricultural Science, Rural Development Administration) ;
  • Yun, Yeo-Hong (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Kim, Seong-Hwan (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Shim, Myung-Yong (Institute of Ecological Phytochemistry, Hankyong National University) ;
  • Choi, Chang-Won (Department of Biology and Medicinal Science, Paichai University) ;
  • Kim, Young-Shick (Department of Plant Science and Technology, Sangmyung University)
  • Received : 2011.10.13
  • Accepted : 2011.11.20
  • Published : 2012.03.01
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Abstract

For the genetic differentiation of $Pseudomonas$ $syringae$ pathovar $tomato$, a total of 51 $P.$ $syringae$ pv. strains infecting 33 different host plants were analyzed using repetitive element PCR(REP-PCR) and universal rice primer PCR(URP-PCR). The entire DNA fingerprint profiles were analyzed using unweighted pair-group method with arithmetic averages (UPGMA). The 51 $P.$ $syringae$ pv. strains could be divided into five clusters based on 65% similarity by Rep-PCR using BOX, ERIC, and REP primers. $P.$ $syringae$ pv. $tomato$ cluster was well separated from other 31 $P.$ $syringae$ pathovars. $P.$ $syringae$ pv. $tomato$ cluster included only $P.$ $syringae$ pv. $maculicola$ and $P.$ $syringae$ pv. $tomato$. $P.$ $syringae$ pv. $tomato$ strains could be divided into two genetic groups. Meanwhile, the Pseudomonas pv. strains could be divided into four clusters based on 63% similarity by URP-PCR using 2F, 9F, and 17R primers. $P.$ $syringae$ pv. $tomato$ cluster was also well separated from 30 other $P.$ $syringae$ pathovars. In this case, $P.$ $syringae$ pv. $tomato$ cluster included $P.$ $syringae$ pv. $maculicola$, $P.$ $syringae$ pv. $berberidi$, and $P.$ $syringae$ pv. $tomato$. $P.$ $syringae$ pv. $tomato$ strains was also separated into two genetic groups by URP-PCR analysis. Overall, our work revealed that $P.$ $syringae$ pv. $tomato$ can be genetically differentiated from other $P.$ $syringae$ pathovars by the DNA fingerprint profiles of REP-PCR and URP-PCR. We first report that there are two genetically diverged groups in $P.$ $syringae$ pv. $tomato$ strains.

Keywords

References

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