Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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The Inhibitory Effects of $Chrthami$ Semen Oil Pharmacopuncture (CSOP) on Synovial Membranes in Type II Collagen-Induced Arthritis Mice

홍화자유약침이 CIA 모델 생쥐의 윤활관절막 손상 억제에 미치는 영향

  • Back, Seong-Wook (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Kim, Eun-Jung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Hwang, Ji-Hoo (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Yoon, Jong-Hwa (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Lee, Seung-Deok (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Kim, Kap-Sung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University)
  • 백성욱 (동국대학교 한의과대학 침구학교실) ;
  • 김은정 (동국대학교 한의과대학 침구학교실) ;
  • 황지후 (동국대학교 한의과대학 침구학교실) ;
  • 윤종화 (동국대학교 한의과대학 침구학교실) ;
  • 이승덕 (동국대학교 한의과대학 침구학교실) ;
  • 김갑성 (동국대학교 한의과대학 침구학교실)
  • Received : 2012.02.06
  • Accepted : 2012.02.13
  • Published : 2012.02.20
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Abstract

Objectives : The purpose of this study is to observe the inhibitory effects of $Chrthami$ semen oil pharmacopuncture(CSOP) on CIA (collagen-induced arthritis) mice. Materials and Methods : Two types of experiments were conducted: $in$ $vitro$ assay, inhibition of MIF mRNA and TNF-${\alpha}$ mRNA expressions in synovial membranes was observed, and $in$ $vivo$ assay, $1{\mu}{\ell}/kg$ CSOP was injected every day to the left $Weizhong$ ($BL_{40}$) from day 3 to 21 after induction of CIA, and changes in paw edema, apical surface morphology, neovascularization in synovial membranes, fibrosis, pro-inflammatory cytokines production, Th-1 differentiation, and anti-inflammatory effect were investigated. Results : 1. In synoviocytes of the CIA mice treated with CSOP, MIF mRNA and TNF-${\alpha}$ mRNA expressions were down-regulated in a dose-dependent manner. 2. Paw edema of the CIA mice treated with CSOP was diminished. 3. Tissue injury in the synovial membranes, capillary distribution and fibrosis were reduced in CSOP-treated mice. 4. MIF, TNF-${\alpha}$, IL-6, MMP-9 expressions were repressed in CSOP-treated mice during the experiment to observe the inhibitory effect on cytokines production in early stage RA. 5. IL-12 and CD28 were reduced in CSOP-treated mice during the observation of inhibitory effect on Th 1 differentiation. 6. PPAR-${\gamma}$ was increased during the experiment to observe the anti-inflammatory effect of CSOP. Conclusions : The results may suggest that administration treatment using $Chrthami$ semen oil pharmacopuncture decreases the inflammatory response on an Animal Model with CIA.

Keywords

References

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