Archives of Plastic Surgery

  • 제39권6호
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  • Pages.593-599
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  • 2012
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  • 2234-6163(pISSN)
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  • 2234-6171(eISSN)
대한성형외과학회 (Korean Society of Plastic and Reconstructive Surgeons)
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Possibility of Undifferentiated Human Thigh Adipose Stem Cells Differentiating into Functional Hepatocytes

  • Lee, Jong Hoon (Department of Plastic and Reconstructive Surgery, Eulji General Hospital, Eulji University College of Medicine) ;
  • Lee, Kuk Han (Department of Plastic and Reconstructive Surgery, Eulji General Hospital, Eulji University College of Medicine) ;
  • Kim, Min Ho (Eulji Medi-Bio Research Institute, Eulji University) ;
  • Kim, Jun Pyo (CL Aesthetic Clinic) ;
  • Lee, Seung Jae (CL Aesthetic Clinic) ;
  • Yoon, Jinah (Department of Biotechnology, Seoul Women's University)
  • 투고 : 2012.06.29
  • 심사 : 2012.08.29
  • 발행 : 2012.11.15
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초록

Background This study aimed to investigate the possibility of isolating mesenchymal stem cells (MSCs) from human thigh adipose tissue and the ability of human thigh adipose stem cells (HTASCs) to differentiate into hepatocytes. Methods The adipose-derived stem cells (ADSCs) were isolated from thigh adipose tissue. Growth factors, cytokines, and hormones were added to the collagen coated dishes to induce the undifferentiated HTASCs to differentiate into hepatocyte-like cells. To confirm the experimental results, the expression of hepatocyte-specific markers on undifferentiated and differentiated HTASCs was analyzed using reverse transcription polymerase chain reaction and immunocytochemical staining. Differentiation efficiency was evaluated using functional tests such as periodic acid schiff (PAS) staining and detection of the albumin secretion level using enzyme-linked immunosorbent assay (ELISA). Results The majority of the undifferentiated HTASCs were changed into a more polygonal shape showing tight interactions between the cells. The differentiated HTASCs up-regulated mRNA of hepatocyte markers. Immunocytochemical analysis showed that they were intensely stained with anti-albumin antibody compared with undifferentiated HTASCs. PAS staining showed that HTASCs submitted to the hepatocyte differentiation protocol were able to more specifically store glycogen than undifferentiated HTASCs, displaying a purple color in the cytoplasm of the differentiated HTASCs. ELISA analyses showed that differentiated HTASCs could secrete albumin, which is one of the hepatocyte markers. Conclusions MSCs were islolated from human thigh adipose tissue differentiate to heapatocytes. The source of ADSCs is not only abundant abdominal adipose tissue, but also thigh adipose tissue for cell therapy in liver regeneration and tissue regeneration.

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참고문헌

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피인용 문헌

  1. Stem Cells in Liver Regeneration and Their Potential Clinical Applications vol.9, pp.5, 2012, https://doi.org/10.1007/s12015-013-9437-4
  2. Cell Therapy for Wound Healing vol.29, pp.3, 2012, https://doi.org/10.3346/jkms.2014.29.3.311
  3. Injectable Tissue-Engineered Soft Tissue for Tissue Augmentation vol.29, pp.suppl3, 2014, https://doi.org/10.3346/jkms.2014.29.s3.s170
  4. In Vivo Effects of Adipose-Derived Stem Cells in Inducing Neuronal Regeneration in Sprague-Dawley Rats Undergoing Nerve Defect Bridged with Polycaprolactone Nanotubes vol.29, pp.suppl3, 2012, https://doi.org/10.3346/jkms.2014.29.s3.s183
  5. Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells vol.1, pp.4, 2014, https://doi.org/10.7603/s40730-014-0021-6
  6. Consideration of Bone Regeneration Effect of Stem Cells: Comparison of Bone Regeneration Between Bone Marrow Stem Cells and Adipose-Derived Stem Cells vol.25, pp.1, 2012, https://doi.org/10.1097/scs.0000000000000378
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  9. Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation vol.10, pp.None, 2012, https://doi.org/10.2147/ijn.s71925
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