Tuberculosis and Respiratory Diseases

  • 제73권1호
  • /
  • Pages.22-31
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  • 2012
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  • 1738-3536(pISSN)
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  • 2005-6184(eISSN)
대한결핵및호흡기학회 (The Korean Academy of Tuberculosis and Respiratory Diseases)
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The Effect of Post-Treatment N-Acetylcysteine in LPS-Induced Acute Lung Injury of Rats

  • Choi, Jae Sung (Department of Internal Medicine, Clinical Research Institute, Soonchunhyang University College of Medicine) ;
  • Lee, Ho Sung (Department of Internal Medicine, Clinical Research Institute, Soonchunhyang University College of Medicine) ;
  • Seo, Ki Hyun (Department of Internal Medicine, Clinical Research Institute, Soonchunhyang University College of Medicine) ;
  • Na, Ju Ock (Department of Internal Medicine, Clinical Research Institute, Soonchunhyang University College of Medicine) ;
  • Kim, Yong Hoon (Department of Internal Medicine, Clinical Research Institute, Soonchunhyang University College of Medicine) ;
  • Uh, Soo Taek (Department of Internal Medicine, Clinical Research Institute, Soonchunhyang University College of Medicine) ;
  • Park, Choon Sik (Department of Internal Medicine, Clinical Research Institute, Soonchunhyang University College of Medicine) ;
  • Oh, Mee Hye (Department of Pathology, Clinical Research Institute, Soonchunhyang University College of Medicine) ;
  • Lee, Sang Han (Department of Biochemistry, Clinical Research Institute, Soonchunhyang University College of Medicine) ;
  • Kim, Young Tong (Department of Radiology, Clinical Research Institute, Soonchunhyang University College of Medicine)
  • 투고 : 2012.04.16
  • 심사 : 2012.06.02
  • 발행 : 2012.07.30
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초록

Background: Oxidation plays an important role in acute lung injury. This study was conducted in order to elucidate the effect of repetitive post-treatment of N-acetylcysteine (NAC) in lipopolysaccaride (LPS)-induced acute lung injury (ALI) of rats. Methods: Six-week-old male Sprague-Dawley rats were divided into 4 groups. LPS (Escherichia coli 5 mg/kg) was administered intravenously via the tail vein. NAC (20 mg/kg) was injected intraperitoneally 3, 6, and 12 hours after LPS injection. Broncho-alveolar lavage fluid (BALF) and lung tissues were obtained to evaluate the ALI at 24 hours after LPS injection. The concentration of tumor necrosis factor ${\alpha}$ (TNF-${\alpha}$) and interleukin $1{\beta}$ (IL-$1{\beta}$) were measured in BALF. Nuclear factor ${\kappa}B$ (NF-${\kappa}B$), lipid peroxidation (LPO), and myeloperoxidase (MPO) were measured using lung tissues. Micro-computed tomography (micro-CT) images were examined in each group at 72 hours apart from the main experiments in order to observe the delayed effects of NAC. Results: TNF-${\alpha}$ and IL-$1{\beta}$ concentration in BALF were not different between LPS and NAC treatment groups. The concentration of LPO in NAC treatment group was significantly lower than that of LPS group ($5.5{\pm}2.8$ nmol/mL vs. $16.5{\pm}1.6$ nmol/mL) (p=0.001). The activity of MPO in NAC treatment group was significantly lower than that of LPS group ($6.4{\pm}1.8$ unit/g vs. $11.2{\pm}6.3$ unit/g, tissue) (p<0.048). The concentration of NF-${\kappa}B$ in NAC treatment group was significantly lower than that of LPS group ($0.3{\pm}0.1\;ng/{\mu}L$ vs. $0.4{\pm}0.2\;ng/{\mu}L$) (p=0.0001). Micro-CT showed less extent of lung injury in NAC treatment than LPS group. Conclusion: After induction of ALI with lipopolysaccharide, the therapeutic administration of NAC partially attenuated the extent of ALI through the inhibition of NF-${\kappa}B$ activation.

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과제정보

연구 과제 주관 기관 : Soonchunhyang University

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