Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
권호 표지

Optimization of Aqueous Methanol Extraction Condition of Total Polyphenol from Spent $Lycium$ $chinense$ Miller to Develop Feed Additives for Pig

양돈용 사료 첨가제 개발을 위하여 구기자 부산물로부터 메탄올수용액을 이용한 총 폴리페놀 추출조건 최적화

  • Shim, Kwan-Seob (Department of Animal Biotechnology, Chonbuk National University) ;
  • Na, Chong-Sam (Department of Animal Biotechnology, Chonbuk National University) ;
  • Oh, Sung-Jin (Department of Animal Science, Chonbuk National University) ;
  • Choi, Nag-Jin (Department of Animal Science, Chonbuk National University)
  • Received : 2012.02.19
  • Accepted : 2012.03.27
  • Published : 2012.03.31
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Abstract

This study was conducted to develop a functional feed additive for pig with spent $Lycium$ $chinense$ Mill fruit. We investigated the optimum conditions for the extraction of polyphenol from spent $Lycium$ $chinense$ Mill using methanol. Methanol concentration as a solvent for extraction, extraction time and the volume of solvent per a gram of solid (ground spent Lyceum chinense Mill) were selected as parameters. Three levels of parameters were configured according to Box Behnken experiment design, a fractional factorial design, and total 15 trials were employed. Total polyphenol concentration from each trial was used as response from experiment system and effects of parameters on total polyphenol extraction efficiency were determined using response surface model. As a result, all terms in analysis of variance, regression ($p$ = 0.001), linear ($p$ = 0.002), square ($p$ = 0.017) and interaction ($p$ = 0.047) was significant and adjusted determination coefficient ($R^2$) was 94.7%. Total polyphenol extraction efficiency was elevated along increased methanol content and decreased solvent to solid ratio. However extraction time did not affect the efficiency. This study provides a primary information for the optimum extraction conditions to maximize total polyphenol recovery from spent Lycium chinens Mill fruit and this result could be applied to re-use of argo-industrial by-products and to develop of functional feed additives in organic farming.

Keywords

References

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