DOI QR코드

DOI QR Code

Morphological and Molecular Characteristics of the Oak Tree Canker Pathogen, $Annulohypoxylon$ $truncatum$

  • Cha, Jae-Yul (Department of Applied Biology, Gyeongsang National University) ;
  • Heo, Bit-Na (Department of Applied Biology, Gyeongsang National University) ;
  • Ahn, Soo-Jeong (Department of Applied Biology, Gyeongsang National University) ;
  • Gang, Guen-Hye (Department of Applied Biology, Gyeongsang National University) ;
  • Park, Chung-Gyoo (Department of Applied Biology, Gyeongsang National University) ;
  • Kwak, Youn-Sig (Department of Applied Biology, Gyeongsang National University)
  • Received : 2011.12.23
  • Accepted : 2012.01.16
  • Published : 2012.03.31

Abstract

Cankers are localized dead areas in the bark of stems, branches or twigs of many types of trees and shrubs, and are usually caused by fungi. We observed severe canker symptoms in oak trees located in Gyeongnam province in 2011. A total 31 trees were discovered with cankers of varied size, with an average of $48.5{\times}15.2cm$. Black, half-rounded globular mound shaped stromata were associated with the cankers, and the asci of the fungi associated with the cankers were cylindrical shaped with their spore-bearing parts being up to 84 ${\mu}m$ in length. The average fungal ascospores size was $7.59{\times}4.23{\mu}m$. The internal transcribed spacer sequence for the canker causing fungus showed 99% similarity to the sequence of $Annulohypoxylon$ $truncatum$. In this study, the isolated fungus was precisely described and then compared with fungi of similar taxa.

Keywords

References

  1. Whalley AJ. Numerical taxonomy of some species of Hypoxylon. Mycopathologia 1976;59:155-61. https://doi.org/10.1007/BF00627877
  2. Ju YM, Rogers JD. A revision of the genus Hypoxylon. Mycologia Memori No. 20. St. Paul: APS Press; 1996.
  3. Hsieh HM, Ju YM, Rogers JD. Molecular phylogeny of Hypoxylon and closely related genera. Mycologia 2005;97: 844-65. https://doi.org/10.3852/mycologia.97.4.844
  4. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. New York: Academic Press, Inc.; 1990. p. 315-33.
  5. Lee YS, Whalley AJ. The genus Hypoxylon, wood decay fungi. II. Teleomorph of Annulata section. Mycobiology 2000;28:1-4.
  6. Lee YS, Whalley AJ. The genus Hypoxylon, wood decay fungi. I. Teleomorph of Hypoxylon section. Mycobiology 2000;28:5-10.