Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Antitumor and Immunostimulating Activities of $Elfvingia$ $applanata$ Hot Water Extract on Sarcoma 180 Tumor-bearing ICR Mice

  • Shim, Sung-Mi (Division of Life Sciences, University of Incheon) ;
  • Lee, Jae-Seong (Division of Life Sciences, University of Incheon) ;
  • Lee, Tae-Soo (Division of Life Sciences, University of Incheon) ;
  • Lee, U-Youn (Division of Life Sciences, University of Incheon)
  • Received : 2012.03.12
  • Accepted : 2012.03.12
  • Published : 2012.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

$Elfvingia$ $applanata$, a medicinal mushroom belonging to Basidiomycota, has been used in the effort to cure cancers of the esophagus and stomach, and is also known to have inhibitory effects on hepatitis B virus infection. The hot water soluble fraction (as Fr. HW) was extracted from fruiting bodies of the mushroom. $In$ $vitro$ cytotoxicity tests showed that hot water extract was not cytotoxic against cancer cell lines such as Sarcoma 180, HT-29, HepG2, and TR at concentrations of 10-2,000 ${\mu}g/mL$. Intraperitoneal injection with Fr. HW resulted in a life prolongation effect of 45.2% in mice previously inoculated with Sarcoma 180. Treatment of Fr. HW resulted in a 2.53-fold increase in the numbers of murine spleen cells at a concentration of 50 ${\mu}g/mL$, compared with control. Incubation of murine spleen cells with Fr. HW at a concentration of 500 ${\mu}g/mL$ resulted in improved immune-potwntiating activity of B lymphocytes through an 8.3-folds increase in alkaline phosphatase activity, compared with control. Fr. HW generated 12.5 ${\mu}M$ of nitric oxide (NO) when cultured with RAW 264.7, a mouse macrophage cell line, at the concentration of 50 ${\mu}g/mL$, while lipopolysaccharide, a positive control, produced 15.2 ${\mu}M$ of NO. Therefore, the results suggested that antitumor activities of Fr. HW from $E.$ $applanata$ might, in part, be due to host mediated immunostimulating activity.

Keywords

References

  1. Ooi VE, Liu F. Immunomodulation and anti-cancer activity of polysaccharide-protein complexes. Curr Med Chem 2000; 7:715-29. https://doi.org/10.2174/0929867003374705
  2. Chihara G, Hamuro G, Maeda Y, Arai Y, Fukuoka F. Antitumor polysaccharide derived chemically from natural glucan (Pachyman). Nature 1970;225:943-4. https://doi.org/10.1038/225943a0
  3. Wasser SP, Weis AL. Medicinal properties of substances occurring in higher basidiomycetes mushrooms: current perspectives (review). Int J Med Mushrooms 1999;1:31-62.
  4. Wasser SP. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol 2002;60:258-74. https://doi.org/10.1007/s00253-002-1076-7
  5. Komatsu N, Okubo S, Kikumoto S, Kimura K, Saito G. Host-mediated antitumor action of schizophyllan, a glucan produced by Schizophyllum commune. Gann 1969;60:137-44.
  6. Chihara G, Maeda Y, Hamuro J, Sasaki T, Fukuoka F. Inhibition of mouse sarcoma 180 by polysaccharides from Lentinus edodes (Berk.) Sing. Nature 1969;222:687-88. https://doi.org/10.1038/222687a0
  7. Tsukagoshi S, Ophashi F. Protein-bound polysaccharide preparation, PS-K, effective against mouse sarcoma-180 and rat ascites hepatoma AH-13 by oral use. Gann 1974;65:557-8.
  8. Park WH, Lee HD. Medicinal mushrooms in Korea. Seoul: Kyohak Publishing Co., Ltd.; 2002.
  9. Ying JZ, Mao XL, Ma QM, Zong YC, Wen HA. Icones of medicinal fungi from China. Beijing: Science Press; 1988.
  10. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  11. Geran RI, Greenberg NH, MacDonald MM, Schumacher AM, Abbot BJ. Protocols for screening chemical agents and natural products against animal tumors and other biological systems. Cancer Chemother Rep 1972;3:59-61.
  12. Ngamwongsatit P, Banada PP, Panbangred W, Bhunia AK. WST-1-based cell cytotocixity assay as a substitute for MTTbased assay for rapid detection of toxigenic Bacillus species using CHO cell line. J Microbiol Methods 2008; 73:211-5. https://doi.org/10.1016/j.mimet.2008.03.002
  13. Ohno N, Arai Y, Suzuki I, Yadomae T. Induction of alkaline phosphatase activity in murine spleen cells treated with various mitogens. J Phamacobiodyn 1986;9:593-9. https://doi.org/10.1248/bpb1978.9.593
  14. Choi SH, Jun CD, Lee, BS, Park SD, Oh JS, Chung HT. Effect of various extrinsic and intrinsic factors on the nitric oxide production of murine macrophage. Korean J Biol Response Modif 1993;3:15-22.
  15. Shim SM, Im KH, Kim JW, Lee UY, Shim MJ, Lee MW, Lee TS. Studies on immuno-modulatory and antitumor effects of crude polysaccharides extracted from Paecilomyces sinclairii. Kor J Mycol 2003;31:155-60. https://doi.org/10.4489/KJM.2003.31.3.155
  16. Lee GW, Kim HY, Hur H, Lee MW, Shim MJ, Lee UY, Lee TS. Antitumor and immuno-modulatory effect of crude polysaccharides from fruiting body of Tremella aurantialba against mouse sarcoma 180. Kor J Mycol 2008;36:66-74. https://doi.org/10.4489/KJM.2008.36.1.066
  17. Clarkson BD, Burchenal JH. Prelimanary screening of antineoplastic drugs. Prog Clin Cancer 1965;1:625-9.
  18. Li X, Jiao LL, Zhang X, Tian WM, Chen S, Zhang LP. Antitumor and immunomodulating activities of proteoglycans from mycelium of Phellinus nigricans and culture medium. Int Immunopharmacol 2008;8:909-15. https://doi.org/10.1016/j.intimp.2008.02.008
  19. Kim GY, Choi GS, Lee SH, Park YM. Acidic polysaccharide isolated from Phellinus linteus enhances through the upregulation of nitric oxide and tumor necrosis factor-α from peritoneal macrophages. J Ethnopharmacol 2004;95;69-76. https://doi.org/10.1016/j.jep.2004.06.024