Mycobiology

The Korean Society of Mycology (한국균학회)
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Isolation, Identification and Characterization of a Antidementia Acetylcholinesterase Inhibitor-Producing $Yarrowia$ $lipolytica$ S-3

  • Kang, Min-Gu (Department of Life Science and Genetic Engineering, PaiChai University) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, Chungnam National University) ;
  • Choi, Young-Jun (Microbial and Enzymatic Technology Group, Bioprocess Platform, Biotechnology Research Institite, National Research Council of Canada) ;
  • Lee, Jong-Soo (Department of Life Science and Genetic Engineering, PaiChai University)
  • Received : 2012.02.16
  • Accepted : 2012.03.09
  • Published : 2012.03.31
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Abstract

This report describes the isolation and identification of a potent acetylcholinesterase (AChE) inhibitor-producing yeasts. Of 731 species of yeast strain, the S-3 strain was selected as a potent producer of AChE inhibitor. The selected S-3 strain was investigated for its microbiological characteristics. The S-3 strain was found to be short-oval yeast that did not form an ascospore. The strain formed a pseudomycelium and grew in yeast malt medium containing 50% glucose and 10% ethanol. Finally, the S-3 strain was identified by its physiological characteristics and 26S ribosomal DNA sequences as $Yarrowia$ $lipolytica$ S-3.

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References

  1. Alzheimer A. Ueber eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr Psychisch-Gerichtliche Med 1907;64:146-8.
  2. Dugue M, Neugroschl J, Sewell M. Marin D. Review of dementia. Mt Sinai J Med 2003;70:45-53.
  3. Eubanks LM, Rogers CJ, Beuscher AE 4th, Koob GF, Olson AJ, Dickerson TJ, Janda KD. A molecular link between the active component of marijuana and Alzheimer's disease pathology. Mol Pharm 2006;3:773-7. https://doi.org/10.1021/mp060066m
  4. Kaye WH, Sitaram N, Weingartner H, Ebert MH, Smallberg S, Gillin JC. Modest facilitation of memory in dementia with combined lecithin and anticholinesterase treatment. Biol Psychiatry 1982;17:275-80.
  5. Khachaturian ZS. Diagnosis of Alzheimer's disease. Arch Neurol 1985;42:1097-105. https://doi.org/10.1001/archneur.1985.04060100083029
  6. Hasselmo ME. Neuromodulation and cortical function: modeling the physiological basis of behavior. Behav Brain Res 1995;67:1-27. https://doi.org/10.1016/0166-4328(94)00113-T
  7. Brenner GM, Stevens CW. Pharmacology. 2nd ed. Philadelphia, PA: Saunders Elsevier; 2006.
  8. Sussman JL, Harel M, Frolow F, Oefner C, Goldman A, Toker L, Silman I. Atomic structure of acetylcholinesterase from Torpedo californica: a prototypic acetylcholine-binding protein. Science 1991;253:872-9. https://doi.org/10.1126/science.1678899
  9. Kim JH, Lee DH, Jeong SC, Chung KS, Lee JS. Characterization of antihypertensive angiotensin I-converting enzyme inhibitor from Saccharomyces cerevisiae. J Microbiol Biotechnol 2004;14:1318-23.
  10. Jeong SC, Lee DH, Lee JS. Production and characterization of an anti-angiogenic agent from Saccharomyces cerevisiae K-7. J Microbiol Biotechnol 2006;16:1904-11.
  11. Lee DH, Lee DH, Lee JS. Characterization of a new antidementia ${\beta}$-secretase inhibitory peptide from Saccharomyces cerevisiae. Enzyme Microb Technol 2007;42:83-8. https://doi.org/10.1016/j.enzmictec.2007.08.003
  12. Lee JS, Yi SH, Kwon SJ, Ahn C, Yoo JY. Isolation, identification and cultural conditions of yeasts from traditional Meju. Korean J Appl Microbiol Biotechnol 1997;25:435-41.
  13. Hasegawa T. Taxonomy and identification of microorganisms. Tokyo: Hakhae Publishing Center; 1984. p. 153-254.
  14. Sohn HY, Seu JH. Screening and characterization of thermotolerant alcohol-producing yeast. J Microbiol Biotechnol 1994;4:215-21.
  15. Kreger-Van Rij. The yeasts: a taxonomic study. 3rd ed. Amsterdam: Elsevier Science; 1984.
  16. Ellman GL, Courtney KD, Andres V Jr, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961;7:88-95. https://doi.org/10.1016/0006-2952(61)90145-9
  17. Lee DH, Lee JS, Yi SH, Lee JS. Production of the acetylcholinesterase inhibitor from Yarrowia lipolytica S-3. Mycobiology 2008;36:102-5. https://doi.org/10.4489/MYCO.2008.36.2.102
  18. van der Aa Kuhle A, Jesperen L, Glover RL, Diawara B, Jakobsen M. Identification and characterization of Saccharomyces cerevisiae strains isolated from West African sorghum beer. Yeast 2001;18:1069-79. https://doi.org/10.1002/yea.756
  19. van der Aa Kuhle A, Jespersen L. The taxonomic position of Saccharomyces boulardii as evaluated by sequence analysis of the D1/D2 domain of 26S rDNA, the ITS1-5.8S rDNAITS2 region and the mitochondrial cytochrome-c oxidase II gene. Syst Appl Microbiol 2003;26:564-71. https://doi.org/10.1078/072320203770865873
  20. Lee JS, Min GH, Lee JS. Nutritional and physicochemical characteristics of the antidementia acetylcholinesterase-inhibiting methanol extracts from Umbilicaria esculenta. Mycobiology 2009;37:203-6. https://doi.org/10.4489/MYCO.2009.37.3.203
  21. Seo DS, Jang JH, Kim NM, Lee JS. Optimal extraction condition and characterization of antidementia acetylcholinesterase inhibitor from Job's tears (Coix lachrymajobi L.). Korean J Med Crop Sci 2009;17:434-8.
  22. Song JE, Song JH, Cho SM, Min GH, Lee JS. Nutritional characteristics and physiological functionality of antidementia acetylcholinesterase inhibitor-containing methanol extract from Sorghum bicolor. Korean J Food Nutr 2010;23:226-32.
  23. Lee EN, Song JH, Lee JS. Screening of a potent antidementia acetylcholinesterase inhibitor-containing fruits and optimal extraction conditions. Korean J Food Nutr 2010;23:318-23.
  24. Jang CH, Eun JS, Park HW, Seo SM, Yang JH, Leem KH, Oh SH, Oh CH, Baek NI, Kim DK. An acetylcholinesterase inhibitor from the leaves of Securinega suffruticosa. Korean J Pharmacogn 2003;34:14-7.