Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Effect of Concentrated Dangyooja-derived Flavonoids Extract Added to Citrus Beverage on Obesity and Blood Lipids in Rats

당유자 유래 Flavonoids 농축액이 첨가된 감귤 음료의 쥐 비만 및 혈중 지질에 미치는 영향

  • Choi, Young-Hun (Citrus Research Station, National Institute of Horticultural & Herbal Science) ;
  • Lee, Young-Jae (College of Veterinary Medicine, Jeju National University) ;
  • Lee, Sun-Yi (Citrus Research Station, National Institute of Horticultural & Herbal Science) ;
  • Chae, Chi-Won (Citrus Research Station, National Institute of Horticultural & Herbal Science) ;
  • Park, Suk-Man (Citrus Research Station, National Institute of Horticultural & Herbal Science) ;
  • Kim, Sang-Suk (Citrus Research Station, National Institute of Horticultural & Herbal Science) ;
  • An, Hyun-Joo (Planning and Coordination Division, National Institute of Horticultural & Herbal Science) ;
  • King, Dale (College of Veterinary Medicine, Jeju National University) ;
  • Han, Chang-Hoon (College of Veterinary Medicine, Jeju National University) ;
  • Hong, Hyun-Ju (College of Veterinary Medicine, Jeju National University)
  • 최영훈 (국립원예특작과학원 감귤시험장) ;
  • 이영재 (제주대학교 수의과대학) ;
  • 이선이 (국립원예특작과학원 감귤시험장) ;
  • 채치원 (국립원예특작과학원 감귤시험장) ;
  • 박석만 (국립원예특작과학원 감귤시험장) ;
  • 김상숙 (국립원예특작과학원 감귤시험장) ;
  • 안현주 (국립원예특작과학원 기획조정과) ;
  • ;
  • 한창훈 (제주대학교 수의과대학) ;
  • 홍현주 (제주대학교 수의과대학)
  • Received : 2011.06.27
  • Accepted : 2012.02.16
  • Published : 2012.04.30
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Abstract

This study aimed to develop a new type of functional citrus beverage (Citurs-F) containing flavonoids extracted from the young fruits of satsuma mandarin ($Citrus$ $unshiu$) and matured fruits of Jeju native dangyooja ($C.$ $grandis$). We made beverages that contained 30% of satsuma mandarin extract with different percentages of concentrated dangyooja-derived flavonoid extracts. In sensory evalution, the highest response indices of color, taste and aroma were from the beverages based on the 30% young fruit extracts plus 15% (Citrus-F-15) and 20% (Citrus-F-20) flavonoids extract from the dangyooja using the KILO prep. In the changes of body weight after oral administration of the Citrus-F, the rat group with HF diet plus the Citrus-F decreased the body weight compared to the rat group fed only HF diet. This effect was to be continued for 9 weeks until the end of experiment. In the lipid content in blood, the rat group with oral administration of citrus extractions merely tended to resolve it in serum test. However, all the 0.1% Citrus-F-15 and Citrus-F-20 treated rat groups from the beginning or after 5 weeks appeared the lowest lipid contents in the blood. In the cholesterol contents, the rat group feeding the KILO-prep's extraction from the beginning weren't significantly recognized them in the group but the rat group feeding 0.1% Citrus-F-15 acted to reduce in the cholesterol contents from 5 weeks. The results indicated that the Citrus-F-15 with rich flavonoids might be main source alleviating the vascular diseases and obesity in human diet.

본 연구에서는 제주산 청과즙과 제주 재래귤인 당유자 과즙 내의 기능성 성분을 대량 추출하여 새로운 타입의 고기능성 감귤 음료를 개발코자 기능성 연구를 실시하였다. 관능 검사를 통해 청과 착즙액 30%에 KILO Prep을 이용하여 얻은 당유자 유래 플라보노이드 농축액 15%를 포함하는 Citrus-F 음료를 제조하였다. 새롭게 제조된 Citrus-F를 경구 투여하여 쥐 체중의 변화를 관찰한 결과 HF diet(고지방사료)와 Citrus-F를 병행하여 급이한 군의 경우 급이 7주차부터 HF diet만 단독으로 급이한 군에 비하여 뚜렷하게 체중이 감소되어 그 효과는 실험이 끝나는 9주째까지 지속되었다. 또한 Citrus-F가 혈중지질에 미치는 영향을 알아보기 위해 경구 투여한 쥐를 대상으로 한 혈청검사에서 혈중 지방 함량은 Citrus-F 처리군에서 감소하는 경향을 보였다. 특히 급이 시점을 초기부터 시작한 경우에는 triglycerid의 수치는 감소하였지만 total cholesterol과 HDL-cholesterol의 수치에는 별다른 영행을 미치지 못했다. 그러나 5주 후부터 0.1% Citrus-F를 급이한 처리군에서 triglyceride, total cholesterol과 HDL-cholesterol의 수치가 모두 감소함을 확인할 수 있었다. 본 연구 결과 당유자 유래 플라보노이드 농축액을 15% 포함하는 고기능성 Citrus-F 음료는 비만 및 각종 혈중 지질 관련 질병의 예방에 효과가 있을 것으로 사료된다.

Keywords

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