원예과학기술지 (Horticultural Science & Technology)

  • 제30권2호
  • /
  • Pages.178-184
  • /
  • 2012
  • /
  • 1226-8763(pISSN)
  • /
  • 2465-8588(eISSN)
한국원예학회 (Korean Society of Horticultural Science)
권호 표지
DOI QR코드

DOI QR Code

ITS 염기서열을 이용한 한국산 참취속 식물의 유연관계분석

Phylogenetic Analysis of Korean Native Aster Plants Based on Internal Transcribed Spacer (ITS) Sequences

  • 홍수영 (농촌진흥청 국립식량과학원 고령지농업연구센터) ;
  • 조광수 (농촌진흥청 국립식량과학원 고령지농업연구센터) ;
  • 유기억 (강원대학교 생명과학과)
  • Hong, Su-Young (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Cho, Kwang-Soo (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Yoo, Ki-Oug (Department of Biological Sciences, Kangwon National University)
  • 투고 : 2011.12.04
  • 심사 : 2012.01.25
  • 발행 : 2012.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 국내산 참취속 식물의 ITS(internal transcribed spacer) 염기서열을 결정하고 이들을 이용하여 $Kalimeris$, $Gymnaster$, $Heteropappus$ 속 식물들간의 유연관계를 분석하고자 수행되었다. 섬쑥부쟁이($A.$ $glehnii$) 등을 포함한 11종의 참취속 식물의 ITS 염기서열을 결정하였으며 유전자 은행에 등록된 $Kalimeris$ 속 식물의 ITS 염기서열을 포함하여 군집분석을 실시하였다. ITS1의 길이는 249-253bp로, ITS2의 길이는 181-217bp로 나타났으며 G + C 함량은 47-54%의 변이를 나타내었다. 본 연구에서 다룬 16종의 18개체간 염기 치환율은 한 site당 ITS1의 경우는 9%, ITS2는 10%로 나타나 ITS1과 2 부위간의 차이가 거의 없는 것으로 나타났다. 또한 협의의 참취속($Aster$ sensu strict) 식물과 $Kalimeris$ 속 식물의 치환율을 비교한 결과 ITS1과 ITS2 지역 모두 $Kalimeris$ 속 식물이 낮게 나타났다. 계통분석결과, 갯개미취는 군외군으로부터 가장 먼저 분지하였으며, 일본산 $A.$ $bellidiastrum$이 나머지 분류군들을 위한 자매군으로 유집되었다. 참취는 다른 참취속 식물들과 분리되어 독립적인 분계조를 형성하였다. 갯쑥부쟁이($A.$ $hispidus$)를 제외한 $Kalimeris$ 속 식물은 91%의 높은 지지율을 가지고 광의의 참취속내로 유집되었으며 $Gymnaster$와 $Heteropappus$ 속 식물 역시 광의의 참취속으로 유집되었다. 이러한 연구결과는 $Kalimeris$, $Gymnaster$ 및 $Heteropappus$로 세분되었던 참취속을 ITS 염기서열을 바탕으로 통합될 수 있을 것으로 판단된다.

This study was carried out to decide ITS (internal transcribed spacer) sequence of some Korean native $Aster$ species and to resolve their relationship among Korean native $Aster$, including $Kalimeris$, $Gymnaster$, $Heteropappus$ genus separated from $Aster$ in a previously study based on the pappus length. We registered 11 ITS sequences of $Aster$ species including $A.$ $glehni$ to GenBank and those sequences were used for the cluster analysis with $Kalimeris$ species. The size of ITS1 was varied from 248 to 256 bp, while ITS2 was varied from 220 to 222 bp. The G + C content of the ITS region ranged from 49.4 to 53.5%. Pairwise comparison results showed that the substitution rate of ITS1 and ITS2 region was 9% and 10%, respectively. $Kalimeris$ sensu strict substitution rate was lower than that of $Aster$ sensu strict species. The strict consensus parsimonious cluster analysis showed $A.$ $tripolium$ is the first branching from the clade and the next is $A.$ $scaber$. The $Kalimeris$ species except for the $A.$ $hispidus$ were grouped into the same clade with high bootstrap value (91%) within $Aster$. $Gymnaster$ and $Heteropappus$ that has been classified by morphological characters were also grouped into broad sense $Aster$ clade. These results implied these three genera could be merged together into $Aster$ based on the ITS sequences.

키워드

참고문헌

  1. Ahn, S.Y., K.S. Cho, K.O. Yoo, and J.T. Suh. 2010. Phylogenetic relationship of Ligularia species based on RAPD and ITS sequence analysis. Kor. J. Hort. Sci. Technol. 28:638-647.
  2. Andreason, K. and B.G. Baldwin. 2003. Reexamination of relationships, habital evolution, and phylogeography of checker mallows (Sidalcea; Malvaceae) based on molecular phylogenetic data. Am. J. Bot. 90:436-444. https://doi.org/10.3732/ajb.90.3.436
  3. Bayer, R.J., D.E. Soltis, and P.S. Soltis. 1996. Phylogenetic inferences in Antennaria (Asteracea: Gnaphalieae: Cassiniinae) based on sequences from nuclear ribosomal DNA internal transcribed spacers. Am. J. Bot. 83:516-527. https://doi.org/10.2307/2446220
  4. Chung, G.Y. and H.J. Jeong. 1999. Study on the leaf morphology of Korean Aster L. and its allied taxa. Korean J. Plant. Res. 12:50-61.
  5. Chung, G.Y. and H.J. Jeong. 2000. Taxonomic study on the achene morphology of Korea Aster L. and its allied taxa. Korean J. Plant. Res. 13: 179-187
  6. Chung, G.Y. and Y.S. Kim. 1991a. A taxonomic study of the pappus morphology on the genus Aster L. and its allied groups in Korea. Korean J. Pl. Taxon. 21:197-209. https://doi.org/10.11110/kjpt.1991.21.3.197
  7. Chung, G.Y. and Y.S. Kim. 1991b. A taxonomic study of the receptacle morphology on the genus Aster L. and its allied groups in Korea. Korean J. Pl. Taxon. 21:229-238. https://doi.org/10.11110/kjpt.1991.21.4.229
  8. Chung, G.Y. and Y.S. Kim. 1993. A study on the capitulum morphology of the genus Aster L. and its allied taxa in Korea. Korean J. Pl. Taxon. 23:105-118. https://doi.org/10.11110/kjpt.1993.23.3.105
  9. Chung, G.Y. and Y.S. Kim. 1997. Study on the somatic chromosome numbers of Korean Aster L. and its allied taxa. Korean J. Plant Res. 10:292-299.
  10. Felsenstain, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:789-791.
  11. Field, B.L., A. Houben, J.N. Timmis, and C.R. Leach. 2006. Internal transcribed spacer sequences analyses indicate cytoevolutionary patterns within Brachycome Cass. (Asteraceae). Plant Syst. Evol. 259:39-51. https://doi.org/10.1007/s00606-005-0400-y
  12. Gu, H. and P.C. Hoch. 1997. Systemacitcs of Kalimeris (Asterceae: Astereae). Annal. Missouri Bot. Gard. 84:762-814 https://doi.org/10.2307/2992027
  13. Huziwara, Y. 1958. Karyotype analysis in some genera of Compositae. IV. The karyotype within the genera Gymnaster, Kalimeris, and Heteropappus. Cytologia 23: 33-45. https://doi.org/10.1508/cytologia.23.33
  14. Ito, M., A. Soejima, M. Hasebe, and K. Watanabe. 1995. A chloroplast-DNA phylogeny of Kalimeris and Aster, with reference to generic circumscription. J. Plant Res. 108:303-304.
  15. Ito, M., A. Soejima, and K. Watanabe. 1998. Phylogenetic relationships of Japanese Aster (Asteraceae, Astereae) sensu lato based on chloroplast-DNA restriction mutations. J. Plant Res. 111:217-223. https://doi.org/10.1007/BF02512173
  16. Kimura, M. 1980. A simple method for estimating evolutionary rate base substitution comparative studies nucleotide sequences. J. Mol. Evol. 16:111-120. https://doi.org/10.1007/BF01731581
  17. Kitamura, S. 1937. Compositae Japonicae. Mem. Coll. Sci. ser. 8:299-377.
  18. Martin, L., C. Oberprieler, and F.H. Hellwig. 2003. A phylogenetic analysis of Primulaceae s.l. based on internal transcribed spacer (ITS) DNA sequence data. Plant Syst. Evol. 237:75-85. https://doi.org/10.1007/s00606-002-0258-1
  19. Masuda, Y., T. Yukawa, and K. Kando. 2009. Molecular phylogenetic analysis of members of Chrysanthemum and its related genera in the tribe Anthemideae, the Asteraceae in East Asia on the basis of the internal transcribed spacer (ITS) region and the external transcribed spacer (ETS) region of nrDNA. Chromosome Botany 4:25-36. https://doi.org/10.3199/iscb.4.25
  20. Matoba, H., A. Soejima, and Y. Hoshi. 2007. Identification of parental genomes and genomic organization in Aster microcephalus var. ovatus. J. Plant Res. 120:585-593. https://doi.org/10.1007/s10265-007-0101-4
  21. Richard, D. and L.H. Rieseberg. 1999. ITS sequence data support a single origin for North American Astereae (Asteraceae) and reflect deep geographic division in Aster. Am. J. Bot. 83:398-412.
  22. Shin, S.K., J.S. Yun, T. Yun, and W.K. Sim. 2003. Taxonomic relationship of Lotus (Nelumbo nucifera) based on ITS sequences of nuclear ribosomal DNA. J. Kor. Soc. Hort. Sci. 44:451-457.
  23. Swofford, D.L. 1998. $PAUP^{\ast}$: Phylogenetic Analysis Using Parsimony and Other Methods. Version 4.02b Sinauer Asso. Inc., Massachusetts, USA.
  24. Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood distance, and maximum parsimony methods. Mol. Biol. Evol. 28:2731-2739. https://doi.org/10.1093/molbev/msr121
  25. Yang, W., B.J. Glover, G.Y. Gao, and J. Yang. 2006. Molecular evidence for multiple polyploidization and lineage recombination in the Chrysanthemum indicum polyploidy complex (Asteraceae). New Phytologist 171:875-886. https://doi.org/10.1111/j.1469-8137.2006.01779.x

피인용 문헌

  1. sequences vol.45, pp.1, 2015, https://doi.org/10.3372/wi.45.45108
  2. Phylogenetic relationships among Egyptian species of Anthemideae (Asteraceae) using ITS markers vol.12, pp.1, 2017, https://doi.org/10.3199/iscb.12.1
  3. 섬쑥부쟁이와 쑥부쟁이의 항산화 및 지방세포 분화 억제 효과 vol.52, pp.3, 2012, https://doi.org/10.4163/jnh.2019.52.3.250