한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제40권4호
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  • Pages.396-402
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  • 2012
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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참쑥(Artemisia dubia) 오일의 돼지 유행성 설사 바이러스(Porcine Epidemic Diarrhea Virus)에 대한 항바이러스 항균활성

Anti-Porcine Epidemic Diarrhea Virus (PEDV) Activity and Antimicrobial Activities of Artemisia dubia Essential Oil

  • 투고 : 2012.08.29
  • 심사 : 2012.10.30
  • 발행 : 2012.12.28
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초록

이 연구에서 참쑥(Artemisia dubia) 오일의 화학적 조성, 돼지 유행성 설사 바이러스(porcine epidemic diarrhea virus, PEDV)에 대한 항바이러스 활성 및 항균 활성을 평가하였다. 참쑥 오일로부터 질량 분석기가 장착된 가스 크로마토 그래피(gas chromatography-mass spectrometry, GC-MS)에 의해 58개의 화합물이 분석되어졌다. 그 오일의 주요한 성분은 각각 camphor (17.18%), germacrene-D (15.70%), trans(${\beta}-$)caryophyllene (6.79%), ${\beta}$-thujones (6.57%), 1, 8-cineole (5.94%) and camphene (5.08%) 순으로 나타났다. 참쑥 오일의 돼지 유행성 설사 바이러스에 대한 항바이러스능을 세포독성 감소 방법 [cytopathic effect (CPE) reduction method]에 의해 베로 세포(원숭이 신장세포, Vero cells)에서 평가하였다. 그 오일은 43.7 ${\mu}g/ml$의 50% 저해농도 ($IC_{50}$)와 함께 돼지 유행성 설사 바이러스 복제를 억제하였다. 또한 그 오일은 100 ${\mu}g/ml$ 이상에서 세포독성을 나타내지 않았으며, 그 치료지수(therapeutic index, TI value)는 2.3 이상이였다. 리바비린(rivabirin)은 그 오일에 비해 상대적으로 낮은 항바이러스능을 나타내었다. 다섯개 미생물(Staphyloscoccus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Propionibacterium acnes, Candida albicans)에 대한 참쑥 오일의 항균 활성은 페이퍼 디스크 방법(paer disck diffusion method)에 의해 평가하였다. 그 결과 그 오일은 5가지 미생물에 대해 8-22 mm의 투명환(clear zone)을 나타내며 항균활성을 보였다. 테스트된 미생물들 가운데 S. pyogenes가 가장 높은 활성을 나타냈으며, C. albicans가 가장 낮은 활성을 나타냈다. 이상의 결과로부터 참쑥 오일은 항균 및 돼지 유행성 설사 바이러스 유래 질병을 조절하는데 응용 가능할 것이라 사료된다.

The chemical composition, anti-porcine epidemic diarrhea virus (PEDV) activity and antimicrobial activity of Artemisia dubia essential oil were evaluated in this study. Fifty eight compounds from A. dubia essential oil were identified through analysis by gas chromatography-mass spectrometry (GC-MS). The major constituents of the oil were camphor (17.18 %), germacrene-D (15.70%), trans (${\beta}-$) racaryophyllene (6.79%), ene thujones (6.57%), 1, 8-cineole (5.94%) and camphene (5.08%). The essential oil was evaluated for antiviral activity against PEDV in Vero cells using a cytopathic effect (CPE) reduction method. The oils actively inhibited PEDV replication with a 50% inhibitory concentration ($IC_{50}$) of 43.7 ${\mu}^3/mL$. The 50% cytotoxicity concentration ($CC_{50}$) of the oils was over 100 ${\mu}/mL$ and the derived therapeutic index was >2.3. Similar analysis of the ribavirin revealed that they have a relatively weaker efficacy when compared to the oils. The antimicrobial activity of the essential oil against 5 microorganisms was evaluated by the disc diffusion method. The essential oil exhibited antimicrobial activity against 5 tested microorganisms with a clear zone of 8-22 mm. Among the tested microorganisms, Streptococcus pyogenes was the most sensitive and Candida albicans the least. Therefore, in can be concluded that essential oils of A. dubia may have interesting applications for microbial control or the control of PEDV-derived diseases.

키워드

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피인용 문헌

  1. Chemical Profile and Biological Activities of Essential Oil from Flowers of Artemisia dubia Wall. ex Bess. Growing Wild in Western Himalaya, India vol.31, pp.8, 2019, https://doi.org/10.14233/ajchem.2019.21970