Journal of Microbiology and Biotechnology

  • 제22권10호
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  • Pages.1446-1451
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  • 2012
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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A Study on the Electrochemical Synthesis of L-DOPA Using Oxidoreductase Enzymes: Optimization of an Electrochemical Process

  • Rahman, Siti Fauziyah (Interdisciplinary Program of Bioenergy and Biomaterial Engineering, Chonnam National University) ;
  • Gobikrishnan, Sriramulu (Interdisciplinary Program of Bioenergy and Biomaterial Engineering, Chonnam National University) ;
  • Indrawan, Natarianto (Interdisciplinary Program of Bioenergy and Biomaterial Engineering, Chonnam National University) ;
  • Park, Seok-Hwan (Interdisciplinary Program of Bioenergy and Biomaterial Engineering, Chonnam National University) ;
  • Park, Jae-Hee (Interdisciplinary Program of Bioenergy and Biomaterial Engineering, Chonnam National University) ;
  • Min, Kyoungseon (Clean Energy Research Center, Korea Institute of Science and Technology) ;
  • Yoo, Young Je (School of Chemical and Biological Engineering, Seoul National University) ;
  • Park, Don-Hee (Interdisciplinary Program of Bioenergy and Biomaterial Engineering, Chonnam National University)
  • 투고 : 2012.06.19
  • 심사 : 2012.07.18
  • 발행 : 2012.10.28
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초록

Levodopa or L-3,4-dihydroxyphenylalanine (L-DOPA) is the precursor of the neurotransmitter dopamine. L-DOPA is a famous treatment for Parkinson's disease symptoms. In this study, electroenzymatic synthesis of L-DOPA was performed in a three-electrode cell, comprising a Ag/AgCl reference electrode, a platinum wire auxiliary electrode, and a glassy carbon working electrode. L-DOPA had an oxidation peak at 376 mV and a reduction peak at -550 mV. The optimum conditions of pH, temperature, and amount of free tyrosinase enzyme were pH 7, $30^{\circ}C$, and 250 IU, respectively. The kinetic constant of the free tyrosinase enzyme was found for both cresolase and catacholase activity to be 0.25 and 0.4 mM, respectively. A cyclic voltammogram was used to investigate the electron transfer rate constant. The mean heterogeneous electron transfer rate ($k_e$) was $5.8{\times}10^{-4}$ cm/s. The results suggest that the electroenzymatic method could be an alternative way to produce L-DOPA without the use of a reducing agent such as ascorbic acid.

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참고문헌

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피인용 문헌

  1. Overview on the biotechnological production of l-DOPA vol.99, pp.2, 2012, https://doi.org/10.1007/s00253-014-6215-4
  2. L-DOPA Synthesis Using Tyrosinase-immobilized on Electrode Surfaces vol.54, pp.6, 2012, https://doi.org/10.9713/kcer.2016.54.6.817
  3. Effective L-Tyrosine Hydroxylation by Native and Immobilized Tyrosinase vol.11, pp.10, 2012, https://doi.org/10.1371/journal.pone.0164213
  4. Electrochemical Screening and Evaluation of Lamiaceae Plant Species from South Africa with Potential Tyrosinase Activity vol.19, pp.5, 2012, https://doi.org/10.3390/s19051035
  5. Fundamentals, Applications, and Future Directions of Bioelectrocatalysis vol.120, pp.23, 2012, https://doi.org/10.1021/acs.chemrev.0c00472