Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Proteomic Analysis of Proteins of Weissella confusa 31 Affected by Bile Salts

  • Lee, Kang Wook (Division of Applied Life Science (BK21), Graduate School, Gyeongsang National University) ;
  • Lee, Seung-Gyu (Division of Applied Life Science (BK21), Graduate School, Gyeongsang National University) ;
  • Han, Nam Soo (Department of Food Science and Technology, BK21 Education and Research Center for Advanced Bio-Agriculture Technology, Chungbuk National University) ;
  • Kim, Jeong-Hwan (Division of Applied Life Science (BK21), Graduate School, Gyeongsang National University)
  • Received : 2012.03.29
  • Accepted : 2012.06.11
  • Published : 2012.10.28
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Abstract

Weissella confusa 31, an isolate from human feces, possesses desirable properties as a probiotic strain, including bile salt resistance. W. confusa 31 is not inhibited by bile salts up to 0.3% concentration. Proteins affected by bile salts (0.05%) were examined by 2-D gel electrophoresis. Our proteomic analyses revealed that the intensities of 29 spots were changed, where 17 increased (including 2 spots observed only under the bile salts stress conditions) and 12 decreased. Proteins were identified by MALDI-TOF mass spectrometry. Proteins increased in the band intensities included adenylate kinase (12.75-fold increase), Clp-like ATP-dependent protease (11.91-fold), 6-phosphogluconate dehydrogenase (10.35-fold), and HSP 70 (5.07-fold). Some of the increased or decreased proteins are also known to be involved in other types of stress responses.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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