Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Immobilization of a Mediator onto Carbon Cloth Electrode and Employment of the Modified Electrode to an Electroenzymatic Bioreactor

  • Jeong, Eun-Seon (Department of Food Science and Technology, Chonbuk National University) ;
  • Sathishkumar, Muthuswamy (Singapore-Delft Water Alliance, National University of Singapore) ;
  • Jayabalan, Rasu (Department of Life Science, National Institute of Technology) ;
  • Jeong, Su-Hyeon (Department of Food Science and Technology, Chonbuk National University) ;
  • Park, Song-Yie (Gochang Blackraspberry Research Institute) ;
  • Mun, Sung-Phil (Department of Wood Science and Technology, Institute of Agricultural Science and Technology, Chonbuk National University) ;
  • Yun, Sei-Eok (Department of Food Science and Technology, Chonbuk National University)
  • Received : 2012.02.24
  • Accepted : 2012.06.20
  • Published : 2012.10.28
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Abstract

5,5'-Dithiobis(2-nitrobenzoic acid) (DTNB) was selected as an electron transfer mediator and was covalently immobilized onto high porosity carbon cloth to employ as a working electrode in an electrochemical $NAD^+$-regeneration process, which was coupled to an enzymatic reaction. The voltammetric behavior of DTNB attached to carbon cloth resembled that of DTNB in buffered aqueous solution, and the electrocatalytic anodic current grew continuously upon addition of NADH at different concentrations, indicating that DTNB is immobilized to carbon cloth effectively and the immobilized DTNB is active as a soluble one. The bioelectrocatalytic $NAD^+$ regeneration was coupled to the conversion of L-glutamate into ${\alpha}$-ketoglutarate by L-glutamate dehydrogenase within the same microreactor. The conversion at 3 mM monosodium glutamate was very rapid, up to 12 h, to result in 90%, and then slow up to 24 h, showing 94%, followed by slight decrease. Low conversion was shown when substrate concentration exceeding 4 mM was tested, suggesting that L-glutamate dehydrogenase is inhibited by ${\alpha}$-ketoglutarate. However, our electrochemical $NAD^+$ regeneration procedure looks advantageous over the enzymatic procedure using NADH oxidase, from the viewpoint of reaction time to completion.

Keywords

Acknowledgement

Supported by : Chonbuk National University

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