Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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β-Glucanase-assisted extraction of starch from glutinous barley

찰보리 전분 추출에 있어서 β-Glucanase 처리 효과

  • Bae, Jae-Seok (Department of Food Science & Technology, Chungnam National University) ;
  • Lee, Eui-Suk (Department of Food Science & Technology, Chungnam National University) ;
  • Jeong, Yong-Sun (Department of Food Science & Technology, Chungnam National University) ;
  • Kim, Jeong-Won (Department of Food Science & Technology, Chungnam National University) ;
  • Lee, Mi-Ja (National Institute of Crop Science, wintercereal and forage crop research division) ;
  • Hong, Soon-Taek (Department of Food Science & Technology, Chungnam National University)
  • Received : 2012.09.17
  • Accepted : 2012.09.25
  • Published : 2012.09.30
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Abstract

In the present study, ${\beta}$-glucanase-assisted extraction of starch from glutinous barley(Hinchal ssalbory) was investigated. ${\beta}$-glucanase was added to a coarse starch suspension obtained after wet milling in the starch extraction process. It was found that in the isolated starch with enzyme treatment, protein content was lower by 0.03%, compared to that with non-enzyme treatment. More importantly it was observed that the extraction yield of starch from enzyme treatment was found to be about 12% higher than the one from non-enzyme treatment (enzyme treated: 90.56%, non-enzyme treated: 78.46%). In order to elucidate this finding, the mass-balance determination of starch in each extraction step was carried out and found that the enzyme treatment might influence on the insoluble residues(R3 and R4 fractions) to hydrolyze ${\beta}$-glucan and other materials (e.g., mucilages etc.), thereby facilitated the separation of starch from it and a next filtration process. With a phase-contrast microscope it was observed that the isolated starch with enzyme treatment contained small starch granules more than the one with non-enzyme treatment and this might result in higher extraction yield observed with the former. In order to confirm this hypothesis, further experiments would be necessary.

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