Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지
DOI QR코드

DOI QR Code

Relationship between Transferable Embryos and Major Metabolite Concentrations in Holstein Donor Cows

  • Son, Jun-Kyu (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Jung, Yeon-Sub (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Cho, Sang-Rae (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Baek, Kwang-Soo (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Yoon, Ho-Beak (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Lim, Hyun-Joo (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Kwon, Eung-Gi (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Kim, Sang-Bum (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Choe, Changyong (Dairy Science Division, National Institute of Animal Science, RDA)
  • Received : 2012.10.18
  • Accepted : 2012.10.30
  • Published : 2012.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This research was investigated the relationship, in high-producing Holstein donor cows, between the number of the transferable embryos and the blood serum concentrations of Blood Urea Nitrogen (BUN), glucose and cholesterol, which affect the nutritional state of cows. CIDRs were inserted into the vaginas of twenty two heads of Holstein cows, regardless of estrous cycle. Superovulation was induced using folliclar stimulating hormone (FSH). For artificial insemination, donor cows were injected with $PGF_{2{\alpha}}$ and estrus was checked about 48 hours after the injection. Then they were treated with 4 straws of semen 3 times, with 12-hour intervals. Embryos were collected by a non-surgical method 7 days after the first artificial insemination. The total numbers of ova collected from 3 experimental groups whose blood BUN concentrations were <10 mg/dl, 11~18 mg/dl and ${\geq}19$ mg/dl were 8.9, 12.5 and 19.0, respectively; whereas the numbers of transferable embryos were 5.8 + 1.9, 7.9 + 2.8 and 5.2 + 1.4, respectively. When glucose concentration was <60 mg/dl, the total number of collected ova was 9.9, which was smaller than when the concentration was 60~70 mg/dl or ${\geq}70$ mg/dl. When glucose concentration was 60~70 mg/dl, the number of transferable embryos was 7.1 + 2.4, which was slightly larger than the numbers 6.4 + 2.1 and 6.1 + 1.7 that were obtained when the concentrations were <60 mg/dl and ${\geq}70$ mg/dl, respectively ; however, the differences were not significant (p>0.05). When cholesterol concentrations were <150 mg/dl, 150~200 mg/dl and ${\geq}200$ mg/dl, the total numbers of collected ova were 11.2, 11.3 and 8.6, respectively. Whereas the numbers of transferable embryos were 7.1 + 2.1, 7.3 + 1.9 and 5.6 + 1.3, respectively ; however, the differences were again not significant (p>0.05). The result of this research showed no significant difference in ovum recovery rate and the number of transferable embryos according to major metabolite concentrations in high-producing Holstein donor cows. However, it is considered that the failure of maintaining proper nutritional status would cause the fall in in vivo embryo productivity.

Keywords

References

  1. Almeida AP. 1987. Superovulatory response in dairy cows repeatedly treated with PMSG. Theriogenology 27:205. https://doi.org/10.1016/0093-691X(87)90082-3
  2. Armstrong DT. 1993. Recent advances in superovulation of cattle. Theriogenology 28:531-540.
  3. Bader JF, Kojima FN, Wehrman ME, Lidsey BR, Kerley MS and Patteson DJ. 2005. Effects of prepartum lipid supplementation on FSH superstimulation and transferable embryo recovery in multiparous beef cows. Anim. Reprod. Sci. 85:61-70. https://doi.org/10.1016/j.anireprosci.2004.04.033
  4. Bastidas P and Randel RD. 1987. Seasonal effects on embryo transfer results in Brahman cows. Theriogenology 28:531-540. https://doi.org/10.1016/0093-691X(87)90258-5
  5. Butler WR, Calaman JJ and Beam AW. 1996. Plasma and milk urea nitrogen in relation to pregnancy rate in lactating dairy cattle. J. Anim. Sci. 74: 858-865. https://doi.org/10.2527/1996.744858x
  6. Canfield RW, Sniffen CJ and Butler WR. 1990. Effects of ex cess degradable protein on postpartum reproduction and energy balance in dairy cattle. J. Dairy Sci. 73:2342-2349. https://doi.org/10.3168/jds.S0022-0302(90)78916-3
  7. Carroll DJ, Barten GW, Anderson GW and Smith RD. 1988. Influence of protein intake and feeding stratege on reproductive performance of dairy cows. J. Dairy Sci. 71:3470-3478. https://doi.org/10.3168/jds.S0022-0302(88)79953-1
  8. Christensen LG. 1991. Use of embryo transfer in future cattle breeding schemes. Theriogenology 35:141-149. https://doi.org/10.1016/0093-691X(91)90153-5
  9. Donaldson LE and Perry B. 1983. Embryo production by repeated superovulation of commercial donor cows. Theriogenology 20:163-168. https://doi.org/10.1016/0093-691X(83)90211-X
  10. Donaldson LE. 1984. Effect of age of donor cows on embryo production. Theriogenology 21:963-967. https://doi.org/10.1016/0093-691X(84)90390-X
  11. Elsden RP, Nelson LD and Seidel GE. 1978. Superovulating cows with follicle stimulating hormone and pregnant mare's serum gonadotrophin. Theriogenology 9:17-26. https://doi.org/10.1016/0093-691X(78)90049-3
  12. Ferguson JD, Galligan DT, Blanchard T and Reeves N. 1993. Serum urea nitrogen and conception rate : The usefulness of test information. J. Dairy Sci. 76:3742-3746. https://doi.org/10.3168/jds.S0022-0302(93)77716-4
  13. Goulding D, Williams DH, Roche JF and Boland MP. 1991. superovulation in heifers using pregnant mare's serum gonadotropin or follicle stimulating hormone during the mid luteal stage of the estrus cycle. Theriogenology 36:949-958. https://doi.org/10.1016/0093-691X(91)90320-D
  14. Greve T, Callesen H and Hyttel P. 1983. Endocrine profiles and egg quality in the superovulated cow. Nord. Vet. Med. 35:408-421.
  15. Hasler JF, Brooke GP and McCauley AD. 1981. The relationship between age and response to superovulation in Holstein cows and heifers. Theriogenology 15:109. https://doi.org/10.1016/S0093-691X(81)80026-X
  16. Isogai T, Shimohira I and Kimura K. 1993. Factors affecting embryo prodution following repeated superovulation treatment in Holstein donors. J. Rerpod. Dev. 39:79-84. https://doi.org/10.1262/jrd.39.79
  17. Kim HR, Kim DI, Won YS, Kim CK, Chung YC, Lee KS and Suh KW. 1997. Studies on in vivo embryo production by $FSH-P^{(R)}$and $SUPER-OV^{(R)}$ in Korean native cattle. I. The factors influencing in vivo embryo production by condition of superovulation treatment. K. J. Emb. Trans. 12:37-47.
  18. Lee WY, Song KY, Lim KT , Lee SJ, Lee BC and Jang G. 2012. Influence of factors during superovulation on embryo production in Korean Holstein cattle. J. Vet. Med. Sci. 74(2): 167-174. https://doi.org/10.1292/jvms.11-0057
  19. Lim KT. 2009. Effects of milk production, postparient days or seasons on in vivo embryo production by superovulation in Holstein cows. K. J. Emb. Trans. 24:33-37.
  20. Lim SK, Won JS, Jean GJ, Chang SS, Kang SW, Yun SK and Son DS. 1998. Superovulation in Korea cattle with a single subcutaneous injection of folltropin-V dissolved in polyethyleneglycol. K. J. Emb. Trans. 13:207-212.
  21. Lucy MC. 2001. Reproductive loss in high producing dairy cattle: Where will it end? J. Dairy Sci. 84:1277-1293. https://doi.org/10.3168/jds.S0022-0302(01)70158-0
  22. Park SB, Kim HS, Kim CK, Chung YC, Lee JW and Kim CH. 1997. Relation of conception rate and plasma urea nitrogen in dairy cattle. K. J. Anim. Reprod. 21:185-189.
  23. Pawlyshyn V, Lindsell CE, Braithwaite M and Mapletoft RJ. 1986. Superovulation of beef cows with FSH-P : A doseresponse trial. Theriogenology 25:179. https://doi.org/10.1016/0093-691X(86)90233-5
  24. Pelssier CL. 1976. Dairy cattle breeding problems and their consequence. Theriogenology 6:575-583. https://doi.org/10.1016/0093-691X(76)90125-4
  25. Pradhan R, Oshima K, Oshima Y, Kojima T, Yamamoto N, Ghanem ME, and Nakagoshi N. 2008. Effect of total cholesterol, glucose and blood urea nitrogen on embryo quality in post-partum superovulated suckling Japanese Black cattle. Reprod. Med. and Bio. 7: 55-62. https://doi.org/10.1111/j.1447-0578.2008.00201.x
  26. Roche JF, Mackey D and Diskin MD. 2000. Reproductive management of postpartum cows. Anim. Reprod. Sci. 60:703-712.
  27. Seidel GE. 1981. Superovulation and embryo transfer in cattle. Science 211:351-358. https://doi.org/10.1126/science.7194504
  28. Shea BF, Janzen RE and McDerm DP. 1984. Seasonal variation in response to stimulation and related embryo transfer procedures in Alberta over a nine year period. Theriogenology 21:186-195. https://doi.org/10.1016/0093-691X(84)90316-9
  29. Smith C. 1984. Genetic improvement of livestock, using nucleus breeding units. World Animal Review 65:2-10.
  30. Smith C. 1988. Application of embryo transfer in animal breeding. Theriogenology 29:203-212. https://doi.org/10.1016/0093-691X(88)90040-4
  31. Son DS, Han MH, Choe CY, Choi SH, Cho SR, Kim HJ, Ryu IS, Choi SB, Lee SS, Kim YK, Kim SK, Kim SH, Shin KH and Kim IH. 2006. Embryo production in superior Hanwoo donors and embryo transfer. K. J. Emb. Trans. 21: 147-156.
  32. Son DS, Kim IH, Lee HJ, Suh KH, Lee DW, Ryu IS, Lee KW, Chun KJ, Son SK and Choe SY. 1997. Studies on embryo cryopreservation and twinning by embryo transfer of Korean native cattle. I. Transfer of frozen-thawed embryo and production of calve. K. J. Emb. Trans. 12:75-90.
  33. Son DS, Kim IH, Ryu IS, Yeon SH, Suh KH, Lee DW, Choi SH, Park SB, Lee CS, Choi YI, Ahn BS and Kim JS. 2000. Embryo production and transfer for dairy MOET scheme application. K. J. Emb. Trans. 15:57-65.
  34. Son JK, Choe CY, Cho SR, Yeon SH, Choi SH, Kim NT, Jung JW, Kim SJ, Jung YS, Bok NH, You YH and Son DS. 2010. Relationship between superovulation treatment and Blood Urea Nitrogen(BUN) concentration in Hanwoo donors. Korean J. Anim. Reprod. 34(3):201-205.
  35. Staigmiller RB, Bellows RA. Anderson GB, Seidel GE, Foot WD, Menino AR and Wright RW. 1992. Superovulation of cattle with equine pituitary extract and procine FSH. Theriogenology 37:1091-1099. https://doi.org/10.1016/0093-691X(92)90107-3
  36. Stringfellow DA and Seidel SM. 1998. Manual of the International Embryo Transfer Society. 3rd ed International Embryo Transfer Society Inc Illinois, pp. 165-17.
  37. Takedomi T, Aogagi Y, Konish M, Kishi H, Taya K, Watanabe G and Sasamoto S. 1992. Superovulation in Holstein heifers by a single injection of procine FSH dissolved in polyvinylpyrrolidon. Theriogenology 39:327.
  38. Theera R. 2011. Effect of nutrition on reproductive performance of postparturient dairy cows in the tropics: A review. Thai J. Vet. Med. Suppl. 41:103-107.
  39. Velazquez MA. 2011. The role of nutritional supplementation on the outcome of superovulation in cattle. Anim. Reprod. Sci. 126:1-10. https://doi.org/10.1016/j.anireprosci.2011.05.009
  40. Warfield SJ, Seidel GE Jr. and Eldsen RP. 1986. A comparison of two FSH regimens for superovulating cows and heifers. Theriogenology 25:213. https://doi.org/10.1016/0093-691X(86)90267-0
  41. Yamamoto M, Ooe M, Kawaguchi M and Suzuki T. 1994. Superovulation in the cow with a single intramuscular injection of FSH dissoived in polyvinypyrrolidone. Theriogenology 41:747-755. https://doi.org/10.1016/0093-691X(94)90184-K