Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Efficient Production of Parthenogenetic Murine Embryonic Stem Cells by the Treatment of Pluripotin (SC-1)

Pluripotin(SC-1) 처리를 통한 단위발생 마우스 배아줄기세포 생산 효율 향상

  • Kang, Hoin (Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute and CLS21, Seoul National University School of Dentistry) ;
  • Roh, Sangho (Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute and CLS21, Seoul National University School of Dentistry)
  • 강호인 (서울대학교 치의학대학원 세포재프로그램 및 발생공학 연구실) ;
  • 노상호 (서울대학교 치의학대학원 세포재프로그램 및 발생공학 연구실)
  • Received : 2012.08.19
  • Accepted : 2012.09.03
  • Published : 2012.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Various small molecules can be used to control major signaling pathways to enhance stemness and inhibit differentiation in murine embryonic stem cell (mESC) culture. Small molecules inhibiting the fibroblast growth factor (FGF)/ERK pathway can preserve pluripotent cells from stimulation of differentiation. In this study, we aimed to evaluate the effect of pluripotin (SC-1), an inhibitor of the FGF/ERK pathway, on the colony formation of outgrowing presumptive mESCs. After plating the zona pellucida-free blastocyst on the feeder layer, attached cell clumps was cultured with SC-1 until the endpoint of the experiment at passage 10. In this experiment, when the number of colonies was counted at passage 3, SC-1-treated group showed 3.4 fold more mESC colonies when compared with control group. However, after passage 4, there was no stimulating effect of SC-1 on the colony formation. In conclusion, SC-1 treatment can be used to promote mESC generation by increasing the number of early mESC colonies.

Keywords

References

  1. Chen S, Do JT, Zhang Q, Yao S, Yan F, Peters EC, Schöler HR, Schultz PG and Ding S. 2006. Self-renewal of embryonic stem cells by a small molecule. Proc. Natl. Acad. Sci. U. S. A. 103:17266-17271. https://doi.org/10.1073/pnas.0608156103
  2. Furue M, Okamoto T, Hayashi Y, Okochi H, Fujimoto M, Myoishi Y, Abe T, Ohnuma K, Sato GH, Asashima M and Sato JD. 2005. Leukemia inhibitory factor as an anti-apoptotic mitogen for pluripotent mouse embryonic stem cells in a serum-free medium without feeder cells. In Vitro Cell Dev. Biol. Anim. 41:19-28. https://doi.org/10.1290/0502010.1
  3. Kang H, Sung J, Jung HM, Woo KM, Hong SD and Roh S. 2012. Insulin-like growth factor 2 promotes osteogenic cell differentiation in the parthenogenetic murine embryonic stem cells. Tissue Eng. Part A. 18:331-341. https://doi.org/10.1089/ten.tea.2011.0074
  4. Kawase E, Suemori H, Takahashi N, Okazaki K, Hashimoto K and Nakatsuji N. 1994. Strain difference in establishment of mouse embryonic stem (ES) cell lines. Int. J. Dev. Biol. 38:385-390.
  5. Leitch HG, Blair K, Mansfield W, Ayetey H, Humphreys P, Nichols J, Surani MA and Smith A. 2010. Embryonic germ cells from mice and rats exhibit properties consistent with a generic pluripotent ground state. Development 137:2279- 2287. https://doi.org/10.1242/dev.050427
  6. Li W and Ding S. 2010. Small molecules that modulate embryonic stem cell fate and somatic cell reprogramming. Trends Pharmacol. Sci. 31:36-45. https://doi.org/10.1016/j.tips.2009.10.002
  7. Niwa H, Burdon T, Chambers I and Smith A. 1998. Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3. Genes Dev.12:2048-2060. https://doi.org/10.1101/gad.12.13.2048
  8. Qi X, Li TG, Hao J, Hu J, Wang J, Simmons H, Miura S, Mishina Y and Zhao GQ. 2004. BMP4 supports self-renewal of embryonic stem cells by inhibiting mitogen-activated protein kinase pathways. Proc. Natl. Acad. Sci. U. S. A. 101:6027-6032. https://doi.org/10.1073/pnas.0401367101
  9. Van der Jeught M, O'Leary T, Ghimire S, Lierman S, Duggal G, Versieren K, Deforce D, Chuva de Sousa Lopes S, Heindryckx B and De Sutter P. 2012. The combination of inhibitors of FGF/MEK/Erk and GSK3$\beta$ signaling increases the number of OCT3/4- and NANOG-positive cells in the human inner cell mass, but does not improve stem cell derivation. Stem Cells Dev. Published online (DOI: 10.1089/ scd.2012.0256).
  10. Versieren K, Van der Jeught M, O'Leary T, Duggal G, Gerris J, Chuva de Sousa Lopes S, Heindryckx B and De Sutter P. 2012. Effect of small molecule supplements during in vitro culture of mouse zygotes and parthenogenetic embryos on hypoblast formation and stem cell derivation. Stem Cell Rev. Published online (DOI: 10.1007/s12015-012-9382-7).
  11. Ying QL, Nichols J, Chambers I and Smith A. 2003. BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3. Cell 115:281-292. https://doi.org/10.1016/S0092-8674(03)00847-X