Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Effect of Low Density Lipoprotein (LDL) on Motility, Viability, Membrane Integrity and Acrosome Integrity of Frozen-thawed Sperm in Korean Jeju Black Bull

제주흑우 동결정액 제조 시 Low Density Lipoprotein (LDL)의 첨가가 동결 융해 후 정자의 성상에 미치는 영향

  • Oh, Shin-Ae (National Institute of Animal Science, RDA) ;
  • Choi, Sun-Ho (National Institute of Animal Science, RDA) ;
  • Ko, Min-Hee (National Institute of Animal Science, RDA) ;
  • Kang, Tae-Young (National Institute of Animal Science, RDA) ;
  • Oh, Young-Mi (Department of Veterinary Medicine, Jeju National University) ;
  • Chung, Young-Ho (Department of Companion Animal and Animal Resources Science, Joongbu University) ;
  • Cho, Won-Mo (National Institute of Animal Science, RDA)
  • 오신애 (농촌진흥청 국립축산과학원) ;
  • 최선호 (농촌진흥청 국립축산과학원) ;
  • 고민희 (농촌진흥청 국립축산과학원) ;
  • 강태영 (농촌진흥청 국립축산과학원) ;
  • 오영미 (국립제주대학교 수의과대학) ;
  • 정영호 (중부대학교 애완동물자원학과) ;
  • 조원모 (농촌진흥청 국립축산과학원)
  • Received : 2012.04.19
  • Accepted : 2012.06.18
  • Published : 2012.09.30
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Abstract

This study was designed to determine whether low-density lipoporoteins (LDL) extracted from egg yolk in extender improve the function of Korean Jeju Black Bull semen. The semen was cryopreserved with 5% ethylene glycol (EG) or 7% glycerol (G) extenders containing 10% egg yolk (EY), 4% LDL and 5% EY or 8% LDL. Frozen-thawed sperm were evaluated sperm motility, viability, membrane integrity and acrosome integrity. Post-thawed sperm motility has been significantly higher (p<0.05) in 4% LDL + 5% EY ($69.00%{\pm}4.18$; EG and $63.00%{\pm}9.75$; 7% G) than 8% LDL ($57.00%{\pm}5.70$; EG and $52.00%{\pm}4.47$;G). Treatment of 4% LDL + 5% EY-EG ($66.85%{\pm}5.06$) has been significantly improved sperm viability compared to other treatments except 10% EY - EG. Moreover, in membrane integrity, swollen sperm ratio has been only significantly increased (p<0.05) in 4% LDL + 5% EY - EG ($64.65%{\pm}6.10$) among all treatments. In assess to detect acrosome integrity, especially, AR pattern ratio has been significantly decreased (p<0.05) in 4% LDL + 5% EY - EG among all treatments. In sperm viability as time passes, between 4% LDL + 5% EY and 10% EY, there was no significant difference, but 8% LDL was significantly decreased sperm viability in EG (1 and 2 hrs) and G (30 min, 1, 2, 5 and 12 hrs) extender. However, there were no significant differences among all treatments except 8% LDL-G in sperm membrane integrity. 8% LDL-G has been significantly decreased swollen sperm ratio at 5 hrs after thawed. It is concluded from these results that 4% LDL + 5% EY to the freezing extender showed more positive effect on the frozen-thawed spermatozoa in Korean Jeju Black bull.

Keywords

References

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