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Effect of Peripheral Administration of Kisspeptin-10 on Dynamic LH Secretion in Prepubertal Ewes

  • Wang, Jun (College of Animal Science and Technology, Jilin Agricultural University) ;
  • Sun, Lei (College of Animal Husbandry and Veterinary Medicine, Liaoning Medical University) ;
  • Zhang, Tao (College of Animal Science and Technology, Jilin Agricultural University) ;
  • Zhou, Haizhu (College of Animal Science and Technology, Jilin Agricultural University) ;
  • Lou, Yujie (College of Animal Science and Technology, Jilin Agricultural University)
  • Received : 2011.10.25
  • Accepted : 2012.02.05
  • Published : 2012.06.01

Abstract

The aim of the present study was to clarify the effect of kisspeptin-10 on LH secretion in prepubertal ewes. In experiment 1, prepubertal ewes fitted with indwelling jugular catheters were randomly assigned to receive 0, 0.5, 1 or 2 mg of kisspeptin-10 dissolved in saline, and serial blood samples were collected at 15-min intervals for 180 min to analyze the response curves of LH after injection. In experiment 2, prepubertal ewes fitted with indwelling jugular catheters were injected with 0 or 1 mg of kisspeptin-10 dissolved in saline and the injection was repeated 3 times at 1 h interval and serial blood samples were collected at 15-min intervals for 210 min to analyze the response curves of LH after injection. The results showed that single intravenous administration of 0.5, 1 and 2 mg of kisspeptin-10 all could significantly increased LH secretion in prepubertal ewes, and the effect of 1 and 2 mg of kisspeptin-10 on LH secretion was higher than that of 0.5 mg group. The results also showed that repeated intravenous administration of kisspeptin-10 could effectively increase LH secretion and repeated administration did not influence the effect of kisspeptin-10 on LH secretion in prepubertal ewe. In conclusion, the present study indicated that single or repeated intravenous administration of kisspeptin-10 could effectively increase LH secretion in prepubertal ewes.

Keywords

References

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